Analysis Tools
cellular
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• MEFs exhibit an increase in nuclear volume, but a more compact morphology as measured by shape factor
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• mitotic cells from MEFs have a significant increase in chromosomal bridges, rearrangements, fragmented ends, multiple tri-radial chromosomes, and endoreduplication
• MEFs exhibit decreased genomic integrity (normal spread 47.8%) in contrast to wild-type (normal spread 65.7%)
• MEFs exhibit an increase in the number of pericentric heterochromatin foci as determined by DAPI staining
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polyploidy
(
J:194593
)
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• cell cycle analysis on MEFs indicates an increase in time spent in G2/M phase, decreased time in G0/G1 phase and no change in time spent in S phase
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embryo
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• E12.5 embryos are visibly abnormal and exhibit reduced growth, however, at this time point Mendelian ratios from a heterozygote x heterozygote mating are as expected
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growth/size/body
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• E12.5 embryos are visibly abnormal and exhibit reduced growth, however, at this time point Mendelian ratios from a heterozygote x heterozygote mating are as expected
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mortality/aging
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• numbers of homozygotes produced from a heterozygote x heterozygote mating are significantly less than expected
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reproductive system
