Phenotypes associated with this allele

• Allele Symbol: Pdgfra^{tm1.1(cre/Esr1*)Nshk}
• Allele Name: targeted mutation 1, Shin-Ichi Nishikawa
• Allele ID: MGI:5475226
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pdgfra^tm1.1(cre/Esr1*)Nshk/Pdgfra^tm1.1(cre/Esr1*)Nshk	involves: C57BL/6NCrlj * CBA/JNCrlj	MGI:5490466
cn2	Pdgfra^tm1.1(cre/Esr1*)Nshk/Pdgfra^+ Runx1^tm1Medv/Runx1^tm1Medv	involves: 129P2/OlaHsd * C57BL/6NCrlj * CBA/JNCrlj	MGI:5490465

Genotype
MGI:5490466

hm1

• Allelic Composition: Pdgfra^{tm1.1(cre/Esr1*)Nshk}/Pdgfra^{tm1.1(cre/Esr1*)Nshk}
• Genetic Background: involves: C57BL/6NCrlj * CBA/JNCrlj

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

hematopoietic system phenotype ( J:193389 )

N

Genotype
MGI:5490465

cn2

• Allelic Composition: Pdgfra^{tm1.1(cre/Esr1*)Nshk}/Pdgfra⁺; Runx1^tm1Medv/Runx1^tm1Medv
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6NCrlj * CBA/JNCrlj

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:193389 )

• rescued embryos (tamoxifen treatment at E7.5) do not survive beyond E12.5

hematopoietic system

anemia ( J:193389 )

• rescued embryos (tamoxifen treatment at E7.5) are modestly anemic

abnormal hematopoietic cell number ( J:193389 )

• with tamoxifen treatment at E7.5, Runx1 expression is restored in the Pdgfra+ve mesoderm and this partially restores CD45+ve and Lin-Kit-Sca1+ve (KSL) hematopoietic cells (HPCs) in the fetal liver at E12.5; these populations are absent without tamoxifen treatment

• with tamoxifen treatment at E7.5, partial recovery of colony forming units (CFUs) from liver cells grown on methycellulose is observed, but without tamoxifen, fetal liver cells fail to generate colonies

integument

pallor ( J:193389 )

• rescued embryos (tamoxifen treatment at E7.5) are pale