Phenotypes associated with this allele

• Allele Symbol: Tg(Itgax-EGFP,-cre,-HBEGF,-luc)2Gjh
• Allele Name: transgene insertion 2, Gunter J Hammerling
• Allele ID: MGI:5476442
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Ager^tm1Arnd/Ager^+ Tg(Itgax-EGFP,-cre,-HBEGF,-luc)2Gjh/0	involves: 129P2/OlaHsd * C57BL/6	MGI:5476625
tg2	Tg(Itgax-EGFP,-cre,-HBEGF,-luc)2Gjh/0	C57BL/6-Tg(Itgax-EGFP,-cre,-HBEGF,-luc)2Gjh	MGI:5476623

Genotype
MGI:5476625

cn1

• Allelic Composition: Ager^tm1Arnd/Ager⁺; Tg(Itgax-EGFP,-cre,-HBEGF,-luc)2Gjh/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased dendritic cell number ( J:194800 )

• depletion of conventional dendritic cells after treatment with diphtheria toxin is efficient with over 90% depletion

decreased plasmacytoid dendritic cell number ( J:194800 )

• depletion is less effective than for conventional cells (about 50%)

hematopoietic system

decreased dendritic cell number ( J:194800 )

• depletion of conventional dendritic cells after treatment with diphtheria toxin is efficient with over 90% depletion

decreased plasmacytoid dendritic cell number ( J:194800 )

• depletion is less effective than for conventional cells (about 50%)

Genotype
MGI:5476623

tg2

• Allelic Composition: Tg(Itgax-EGFP,-cre,-HBEGF,-luc)2Gjh/0
• Genetic Background: C57BL/6-Tg(Itgax-EGFP,-cre,-HBEGF,-luc)2Gjh

immune system

immune system phenotype ( J:194800 )

N • 24 hours after 8 or 40 ng/b BW diphtheria toxin injection, mice do not show the early neutrophilia observed in other DTR transgenic animals; no significant increase in intrrenal polymorphonuclear neutrophil (PMN) number is detected and no significant increase in serum Cxcl2 is seen in contrast to Tg(Itgax-DTR/EGFP)57Lan mice after 8 ng/g DT

increased neutrophil cell number ( J:194800 )

• 24 hours after 40 ng/g BW injection of DT, a 66% increase in intrarenal PMNs is seen

• injection of 8 ng/g BW diphtheria toxin causes a 2-fold increase in PMN numbers in blood by 3 days, but not after 24 hours or in homeostasis; level decreases by day 5

increased monocyte cell number ( J:194800 )

• injection of 8 ng/g BW diphtheria toxin causes a 2-fold increase in blood monocyte counts by 3 days; level decreases by day 5

abnormal susceptibility to infection ( J:194800 )

• coli show decreased kidney dendritic cell and PMN numbers and higher UPEC CFUs than nondepleted mice, supporting the theory that improved bacterial clearance is due to the induced neutrophilia in Tg(Itgax-DTR/EGFP)57Lan and Tg(Itgax-DTR/OVA/EGFP)1Garbi mice

hematopoietic system

increased neutrophil cell number ( J:194800 )

• 24 hours after 40 ng/g BW injection of DT, a 66% increase in intrarenal PMNs is seen

• injection of 8 ng/g BW diphtheria toxin causes a 2-fold increase in PMN numbers in blood by 3 days, but not after 24 hours or in homeostasis; level decreases by day 5

increased monocyte cell number ( J:194800 )

• injection of 8 ng/g BW diphtheria toxin causes a 2-fold increase in blood monocyte counts by 3 days; level decreases by day 5