Phenotypes associated with this allele

• Allele Symbol: Usp44^tm1.2Pjgl
• Allele Name: targeted mutation 1.2, Paul J Galardy
• Allele ID: MGI:5478528
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Usp44^tm1.2Pjgl/Usp44^tm1.2Pjgl	involves: 129S4/SvJae * 129S7/SvEvBrd	MGI:5478536
cx2	Mad2l1^tm1Sorg/Mad2l1^+ Usp44^tm1.2Pjgl/Usp44^tm1.2Pjgl	involves: 129S2/SvPas * 129S4/SvJae * 129S7/SvEvBrd	MGI:5478537

Genotype
MGI:5478536

hm1

• Allelic Composition: Usp44^tm1.2Pjgl/Usp44^tm1.2Pjgl
• Genetic Background: involves: 129S4/SvJae * 129S7/SvEvBrd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Usp44^tm1.2Pjgl/Usp44^tm1.2Pjgl MEFs exhibit abnormal spindle geometry

neoplasm

increased tumor incidence ( J:193965 )

• 12 of 24 mice develop at least one tumor type

increased hepatoma incidence ( J:193965 )

increased lung adenoma incidence ( J:193965 )

increased lymphoma incidence ( J:193965 )

increased sarcoma incidence ( J:193965 )

cellular

aneuploidy ( J:193965 )

• in mouse embryonic fibroblasts due to chromosome segregation errors

• in splenocytes in vivo, with increased rates with age

• however, no chromosome breakage is observed

abnormal mitotic spindle morphology ( J:193965 )

• spindle poles do not lie perpendicular to the metaphase plate with a reduction in the inter-pole distance

chromosomal instability ( J:193965 )

• mouse embryonic fibroblasts exhibit increased chromosome segregation errors independent of checkpoints compared with wild-type cells

• APC inhibition does not prevent chromosome mis-segregatio

• however, no chromosome breakage is observed

respiratory system

increased lung adenoma incidence ( J:193965 )

liver/biliary system

increased hepatoma incidence ( J:193965 )

Genotype
MGI:5478537

cx2

• Allelic Composition: Mad2l1^tm1Sorg/Mad2l1⁺; Usp44^tm1.2Pjgl/Usp44^tm1.2Pjgl
• Genetic Background: involves: 129S2/SvPas * 129S4/SvJae * 129S7/SvEvBrd

cellular

chromosomal instability ( J:193965 )

• chromosome instability is not exacerbated compared with cells form Mad2l1^tm1Sorg heterozygotes