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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Eps15tm1Noff
targeted mutation 1, Nina Offenhauser
MGI:5485036
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Eps15tm1Noff/Eps15tm1Noff B6.129-Eps15tm1Noff MGI:5485051
hm2
 		Eps15tm1Noff/Eps15tm1Noff involves: 129 * C57BL/6 MGI:5485052
cx3
 		Eps15tm1Noff/Eps15tm1Noff
Eps15l1tm1.1Noff/Eps15l1tm1.1Noff 		involves: 129 * C57BL/6 MGI:6509032


Genotype
MGI:5485051
hm1
Allelic
Composition		 Eps15tm1Noff/Eps15tm1Noff
Genetic
Background		 B6.129-Eps15tm1Noff
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Eps15tm1Noff mutation (0 available); any Eps15 mutation (66 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:195689 )
N
• marginal zone B cells exhibit normal chemotaxis, proliferation and apoptosis
• mice exhibit a normal B cell immune system response
decreased B cell number ( J:195689 )
• however, total splenic B cells are normal
abnormal spleen white pulp morphology ( J:195689 )
• increased thickness of the cellular rim surrounding the marginal sinus
abnormal spleen B cell follicle morphology ( J:195689 )
• increased marginal zone and follicular area
abnormal spleen marginal zone morphology ( J:195689 )
• increased marginal zone and follicular area
increased marginal zone B cell number ( J:195689 )
• without a change in precursor cells

nervous system
nervous system phenotype ( J:195689 )
N
• mice do not exhibit overt neurological defects

hematopoietic system
decreased B cell number ( J:195689 )
• however, total splenic B cells are normal
abnormal spleen white pulp morphology ( J:195689 )
• increased thickness of the cellular rim surrounding the marginal sinus
abnormal spleen B cell follicle morphology ( J:195689 )
• increased marginal zone and follicular area
abnormal spleen marginal zone morphology ( J:195689 )
• increased marginal zone and follicular area
increased marginal zone B cell number ( J:195689 )
• without a change in precursor cells




Genotype
MGI:5485052
hm2
Allelic
Composition		 Eps15tm1Noff/Eps15tm1Noff
Genetic
Background		 involves: 129 * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Eps15tm1Noff mutation (0 available); any Eps15 mutation (66 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal marginal zone B cell morphology ( J:195689 )
• reconstitution assays reveal that the increase in marginal zone B cells is cell autonomous
increased marginal zone B cell number ( J:195689 )
• at 25, but not 4, months
• however, total splenic B cells are normal

hematopoietic system
abnormal marginal zone B cell morphology ( J:195689 )
• reconstitution assays reveal that the increase in marginal zone B cells is cell autonomous
increased marginal zone B cell number ( J:195689 )
• however, total splenic B cells are normal
• at 25, but not 4, months




Genotype
MGI:6509032
cx3
Allelic
Composition		 Eps15tm1Noff/Eps15tm1Noff
Eps15l1tm1.1Noff/Eps15l1tm1.1Noff
Genetic
Background		 involves: 129 * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Eps15l1tm1.1Noff mutation (0 available); any Eps15l1 mutation (80 available)
Eps15tm1Noff mutation (0 available); any Eps15 mutation (66 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
embryonic lethality during organogenesis, complete penetrance ( J:272122 )
• double homozygous embryos die shortly after E9.5, with no live embryos detected at E11.5-E12.5

embryo
abnormal vitelline vasculature morphology ( J:272122 )
• PECAM staining revealed reduced vascularization of the yolk sac
embryonic growth retardation ( J:272122 )
• at E9.5, some embryos display severe developmental delay
abnormal embryo morphology ( J:272122 )
• embryos exhibit severe morphological defects as early as E9.5
absent limb buds ( J:272122 )
• at E9.5, all embryos exhibit absence of limb buds
fused somites ( J:272122 )
• at E9.5, all embryos exhibit fused somites

cardiovascular system
abnormal vasculogenesis ( J:272122 )
• PECAM staining of the vascular system revealed reduced vascularization of the embryo proper and the yolk sac; detailed confocal analysis confirmed compromised vascularization of the head, somites and yolk sac
abnormal vitelline vasculature morphology ( J:272122 )
• PECAM staining revealed reduced vascularization of the yolk sac
delayed heart looping ( J:272122 )
• at E9.5, all embryos exhibit delayed turning of the heart

integument
pallor ( J:272122 )
• E9.5 embryos appear paler than controls

nervous system
abnormal midbrain-hindbrain boundary morphology ( J:272122 )
• at E9.5, all embryos exhibit a reduced midbrain-hindbrain boundary

limbs/digits/tail
absent limb buds ( J:272122 )
• at E9.5, all embryos exhibit absence of limb buds

growth/size/body
embryonic growth retardation ( J:272122 )
• at E9.5, some embryos display severe developmental delay




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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory