Phenotypes associated with this allele

• Allele Symbol: Yap1^tm1Hmc
• Allele Name: targeted mutation 1, Helen McNeill
• Allele ID: MGI:5486298
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Wwtr1^tm1Hmc/Wwtr1^tm1Hmc Yap1^tm1Hmc/Yap1^tm1Hmc Six2^tm1(tTA,tetO-EGFP/cre)Amc/Six2^+	involves: 129 * 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6J * C57BL/6NCrl	MGI:5486304
cn2	Yap1^tm1Hmc/Yap1^tm1Hmc Six2^tm1(tTA,tetO-EGFP/cre)Amc/Six2^+	involves: 129 * 129S6/SvEvTac * C57BL/6J * C57BL/6NCrl	MGI:5486301
cn3	Rax^tm1.1(cre/ERT2)Sbls/Rax^+ Yap1^tm1Hmc/Yap1^tm1Hmc	involves: 129S6/SvEvTac * C57BL/6NCrl	MGI:6716882

Genotype
MGI:5486304

cn1

• Allelic Composition: Wwtr1^tm1Hmc/Wwtr1^tm1Hmc; Yap1^tm1Hmc/Yap1^tm1Hmc; Six2^{tm1(tTA,tetO-EGFP/cre)Amc}/Six2⁺
• Genetic Background: involves: 129 * 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6J * C57BL/6NCrl

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

renal/urinary system

kidney cyst ( J:195266 )

• some of the few proximal tubules present are cystic

decreased renal glomerulus number ( J:195266 )

abnormal kidney development ( J:195266 )

• developmental defects are similar to mutants wild-type for Wwtr1

decreased renal tubule number ( J:195266 )

growth/size/body

kidney cyst ( J:195266 )

• some of the few proximal tubules present are cystic

Genotype
MGI:5486301

cn2

• Allelic Composition: Yap1^tm1Hmc/Yap1^tm1Hmc; Six2^{tm1(tTA,tetO-EGFP/cre)Amc}/Six2⁺
• Genetic Background: involves: 129 * 129S6/SvEvTac * C57BL/6J * C57BL/6NCrl

Abnormal kidney development in Yap1^tm1Hmc/Yap1^tm1Hmc Six2^{tm1(tTA,tetO-EGFP/cre)Amc}/Six2⁺ mice

mortality/aging

postnatal lethality, complete penetrance ( J:195266 )

• die within 48 hours of birth

renal/urinary system

abnormal kidney cortex morphology ( J:195266 )

• convoluted tubules are not distinguishable in the inner cortex at E18.5

podocyte foot process effacement ( J:195266 )

• seen at P0

abnormal renal glomerulus morphology ( J:195266 )

• few detectable glomeruli

decreased renal glomerulus number ( J:195266 )

• few detectable glomeruli

abnormal kidney development ( J:195266 )

• limited nephrogenesis with abnormal morphogenesis of S shaped bodies at E13.5

• dramatic decrease in the number of nascent nephrons (pretubular aggregates, comma shaped bodies, S shaped bodies, and renal vesicles) at E14.5

abnormal metanephros morphology ( J:195266 )

• abnormal morphogenesis of S shaped bodies at E13.5

• dramatic decrease in the number of nascent nephrons (pretubular aggregates, comma shaped bodies, S shaped bodies, and renal vesicles) at E14.5

• dramatic decrease in the number of cap mesenchyme derived structures that reach the S shaped bodies

• at E14.5 the connecting segment where the S shaped body connects to the ureteric epithelium is abnormal and the distal segment fails to correctly merge with the outermost edge of the ureteric bud

• slight reduction in proliferation in the proximal part of the renal vesicle and the distal part of the S shaped bodies

abnormal nephrogenic zone morphology ( J:195266 )

• reduced nephrogenic zone at E18.5

abnormal kidney medulla morphology ( J:195266 )

• the medulla is mainly composed of collecting ducts at E18.5 suggesting a decrease in the formation of loops of Henle

abnormal kidney papilla morphology ( J:195266 )

• small papilla at E18.5

renal hypoplasia ( J:195266 )

• at P0

abnormal renal tubule morphology ( J:195266 )

• convoluted tubules are not distinguishable in the inner cortex at E18.5

abnormal loop of Henle morphology ( J:195266 )

• defects in formation

abnormal proximal convoluted tubule morphology ( J:195266 )

• few detectable proximal tubules are present

decreased renal tubule number ( J:195266 )

• few detectable proximal tubules are present

impaired branching involved in ureteric bud morphogenesis ( J:195266 )

• decrease in the number of ureteric bud tips at E16.5 and P0 but not at E14.5

anuria ( J:195266 )

• neonatal mice have an empty bladder suggesting a failure to produce urine

Genotype
MGI:6716882

cn3

• Allelic Composition: Rax^{tm1.1(cre/ERT2)Sbls}/Rax⁺; Yap1^tm1Hmc/Yap1^tm1Hmc
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6NCrl

vision/eye

abnormal retina morphology ( J:304126 )

• S-opsin and M-cone opsins are reduced in the retina, with S-opsin signal drastically decreased dorsally while M-opsin almost absent ventrally

• however, the number of cones is not changed

retina gliosis ( J:304126 )

• 4-OHT-treated mice show reactive gliosis in the retina at 12 months of age

nervous system

abnormal glial cell physiology ( J:304126 )

• marker analysis of mice treated with 4-hydroxy-tamoxifen (4-OHT) at P10 shows Muller cell homeostasis dysfunction at 12 months of age