About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Cbx4tm1.2Gxu
targeted mutation 1.2, Guo-Liang Xu
MGI:5489932
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Cbx4tm1.2Gxu/Cbx4tm1.2Gxu involves: 129S2/SvPas * C57BL/6J MGI:5489933
cn2
 		Cbx4tm1.1Gxu/Cbx4tm1.2Gxu
Tg(Foxn1-cre)1Tbo/0 		involves: 129S2/SvPas * C57BL/6J MGI:5489934


Genotype
MGI:5489933
hm1
Allelic
Composition		 Cbx4tm1.2Gxu/Cbx4tm1.2Gxu
Genetic
Background		 involves: 129S2/SvPas * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cbx4tm1.2Gxu mutation (0 available); any Cbx4 mutation (16 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
neonatal lethality, complete penetrance ( J:194053 )
• die within 1 hr of birth

hematopoietic system
hematopoietic system phenotype ( J:194053 )
N
• total numbers of splenocytes and bone marrow cells are similar to wild-type littermates
abnormal thymus development ( J:194053 )
• growth of the thymus is severely retarded after E13.5
• impaired proliferation of thymocytes at E17.5
thymus hypoplasia ( J:194053 )
• thymic hypogenesis, with a decrease in both total thymic cells and thymic epithelial cells
• however, the ratios of the different cell types are similar to controls
abnormal thymus physiology ( J:194053 )
• impaired proliferation of thymocytes at E17.5
• defect in proliferation is due to defect in the stroma, as reconstituted fetal thymic organ cultures using mutant thymi but wild-type hematopoietic progenitor cells show barely detectable numbers of thymocytes

immune system
abnormal thymus development ( J:194053 )
• growth of the thymus is severely retarded after E13.5
• impaired proliferation of thymocytes at E17.5
thymus hypoplasia ( J:194053 )
• thymic hypogenesis, with a decrease in both total thymic cells and thymic epithelial cells
• however, the ratios of the different cell types are similar to controls
abnormal thymus physiology ( J:194053 )
• impaired proliferation of thymocytes at E17.5
• defect in proliferation is due to defect in the stroma, as reconstituted fetal thymic organ cultures using mutant thymi but wild-type hematopoietic progenitor cells show barely detectable numbers of thymocytes

endocrine/exocrine glands
abnormal thymus development ( J:194053 )
• growth of the thymus is severely retarded after E13.5
• impaired proliferation of thymocytes at E17.5
thymus hypoplasia ( J:194053 )
• thymic hypogenesis, with a decrease in both total thymic cells and thymic epithelial cells
• however, the ratios of the different cell types are similar to controls
abnormal thymus physiology ( J:194053 )
• impaired proliferation of thymocytes at E17.5
• defect in proliferation is due to defect in the stroma, as reconstituted fetal thymic organ cultures using mutant thymi but wild-type hematopoietic progenitor cells show barely detectable numbers of thymocytes




Genotype
MGI:5489934
cn2
Allelic
Composition		 Cbx4tm1.1Gxu/Cbx4tm1.2Gxu
Tg(Foxn1-cre)1Tbo/0
Genetic
Background		 involves: 129S2/SvPas * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cbx4tm1.1Gxu mutation (0 available); any Cbx4 mutation (16 available)
Cbx4tm1.2Gxu mutation (0 available); any Cbx4 mutation (16 available)
Tg(Foxn1-cre)1Tbo mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal thymus development ( J:194053 )
• expression analysis indicates a defect in the maturation of both medullary and cortical epithelial cells
thymus atrophy ( J:194053 )
• limited thymic epithelial architecture quickly deteriorates in postnatal mice with discrete cortical and medullary structures no longer seen at 3 weeks of age
abnormal thymus physiology ( J:194053 )
• expression analysis indicates impaired proliferation of developing epithelial cells
abnormal lymphopoiesis ( J:194053 )
• the double negative population is over-represented and composed primarily of B220+ B lineage cells instead of T lineage precursors
• however, the absolute number of B lineage cells in the thymus is similar to controls
decreased T cell number ( J:194053 )
• the T cell compartment in the periphery is never fully established in adult mice
• the CD3+ population in the spleen is small
decreased double-positive T cell number ( J:194053 )
• markedly reduced in proportion in the thymus at 3 weeks of age
• barely detectable at 6 weeks of age

immune system
abnormal thymus development ( J:194053 )
• expression analysis indicates a defect in the maturation of both medullary and cortical epithelial cells
thymus atrophy ( J:194053 )
• limited thymic epithelial architecture quickly deteriorates in postnatal mice with discrete cortical and medullary structures no longer seen at 3 weeks of age
abnormal thymus physiology ( J:194053 )
• expression analysis indicates impaired proliferation of developing epithelial cells
abnormal lymphopoiesis ( J:194053 )
• the double negative population is over-represented and composed primarily of B220+ B lineage cells instead of T lineage precursors
• however, the absolute number of B lineage cells in the thymus is similar to controls
decreased double-positive T cell number ( J:194053 )
• markedly reduced in proportion in the thymus at 3 weeks of age
• barely detectable at 6 weeks of age
decreased T cell number ( J:194053 )
• the T cell compartment in the periphery is never fully established in adult mice
• the CD3+ population in the spleen is small

endocrine/exocrine glands
abnormal thymus development ( J:194053 )
• expression analysis indicates a defect in the maturation of both medullary and cortical epithelial cells
thymus atrophy ( J:194053 )
• limited thymic epithelial architecture quickly deteriorates in postnatal mice with discrete cortical and medullary structures no longer seen at 3 weeks of age
abnormal thymus physiology ( J:194053 )
• expression analysis indicates impaired proliferation of developing epithelial cells




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory