All Phenotypes Sppl2a<tm1Basc> MGI Mouse

Phenotypes associated with this allele

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

Atrophic Peyer's patches in Sppl2a^tm1Basc/Sppl2a^tm1Basc mice

homeostasis/metabolism

abnormal B cell calcium ion homeostasis ( J:194664 )

• calcium ion entry across the plasma membrane of T1 B cells is decreased compared to in wild-type mice

immune system

arrested B cell differentiation ( J:194664 )

• maturation block at T1 stage

• transplant experiments suggest cell-intrinsic defect

decreased B cell number ( J:194664 )

• in the blood, bone marrow, spleen, lymph nodes and peritoneum

decreased transitional stage T2 B cell number ( J:194664 )

• in the spleen

decreased mature B cell number ( J:194664 )

• in the spleen and bone marrow

decreased B-1 B cell number ( J:194664 )

• in the peritoneum

decreased B-2 B cell number ( J:194664 )

• in the peritoneum

decreased follicular B cell number ( J:194664 )

• in the spleen

decreased marginal zone B cell number ( J:194664 )

• in the spleen

decreased T cell number ( J:194664 )

• in the spleen and lymph nodes

small spleen ( J:194664 )

abnormal B cell physiology ( J:194664 )

• B cells accumulate endosomal vacuoles containing Cd74 N terminal fragment compared with wild-type cells

abnormal B cell calcium ion homeostasis ( J:194664 )

• calcium ion entry across the plasma membrane of T1 B cells is decreased compared to in wild-type mice

decreased IgA level ( J:194664 )

• in untreated mice

decreased IgG1 level ( J:194664 )

• in untreated mice

• in mice exposed to TNP-Ficoll or TNP-KHL (after 14 and 28 days)

decreased IgG2a level ( J:194664 )

• in untreated mice

• in mice exposed to TNP-Ficoll or TNP-KHL (after 14 and 28 days)

decreased IgG2b level ( J:194664 )

• in untreated mice

• in mice exposed to TNP-Ficoll or TNP-KHL (after 14 and 28 days)

decreased IgG3 level ( J:194664 )

• in untreated mice

• in mice exposed to TNP-Ficoll or TNP-KHL (after 14 and 28 days)

decreased IgM level ( J:194664 )

• in untreated mice

• in mice exposed to TNP-Ficoll or TNP-KHL (after 14, but not 28, days)

decreased Peyer's patch number ( J:194664 )

small Peyer's patches ( J:194664 )

• atrophic

hematopoietic system

arrested B cell differentiation ( J:194664 )

• maturation block at T1 stage

• transplant experiments suggest cell-intrinsic defect

decreased B cell number ( J:194664 )

• in the blood, bone marrow, spleen, lymph nodes and peritoneum

decreased transitional stage T2 B cell number ( J:194664 )

• in the spleen

decreased mature B cell number ( J:194664 )

• in the spleen and bone marrow

decreased B-1 B cell number ( J:194664 )

• in the peritoneum

decreased B-2 B cell number ( J:194664 )

• in the peritoneum

decreased follicular B cell number ( J:194664 )

• in the spleen

decreased marginal zone B cell number ( J:194664 )

• in the spleen

decreased T cell number ( J:194664 )

• in the spleen and lymph nodes

small spleen ( J:194664 )

abnormal B cell physiology ( J:194664 )

• B cells accumulate endosomal vacuoles containing Cd74 N terminal fragment compared with wild-type cells

abnormal B cell calcium ion homeostasis ( J:194664 )

• calcium ion entry across the plasma membrane of T1 B cells is decreased compared to in wild-type mice

decreased IgA level ( J:194664 )

• in untreated mice

decreased IgG1 level ( J:194664 )

• in untreated mice

• in mice exposed to TNP-Ficoll or TNP-KHL (after 14 and 28 days)

decreased IgG2a level ( J:194664 )

• in untreated mice

• in mice exposed to TNP-Ficoll or TNP-KHL (after 14 and 28 days)

decreased IgG2b level ( J:194664 )

• in untreated mice

• in mice exposed to TNP-Ficoll or TNP-KHL (after 14 and 28 days)

decreased IgG3 level ( J:194664 )

• in untreated mice

• in mice exposed to TNP-Ficoll or TNP-KHL (after 14 and 28 days)

decreased IgM level ( J:194664 )

• in untreated mice

• in mice exposed to TNP-Ficoll or TNP-KHL (after 14, but not 28, days)

Allelic Composition	Cd74^tm1Liz/Cd74^tm1Liz Sppl2a^tm1Basc/Sppl2a^tm1Basc
Genetic Background	B6.129-Sppl2a^tm1Basc Cd74^tm1Liz

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd74^tm1Liz mutation (4 available); any Cd74 mutation (36 available)
Sppl2a^tm1Basc mutation (0 available); any Sppl2a mutation (47 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

Sppl2a^tm1Basc/Sppl2a^tm1Basc Cd74^tm1Liz/Cd74^tm1Liz B cells show normal morphology compared to the increased vacuoles in Sppl2a^tm1Basc/Sppl2a^tm1Basc B cells

immune system

immune system phenotype ( J:194664 )

N	• B cells exhibit the same number of vacuoles per cells as wild-type mice and Cd74^tm1Liz homozygotes • calcium ion flux in T1 B cells is restored to normal • B cell maturation and function are restored • the size of Peyer's patches is restored

decreased B cell number ( J:194664 )

• compared with wild-type mice but improved from Sppl2a^tm1Basc homozygotes in the spleen and lymph nodes

decreased Peyer's patch number ( J:194664 )

• compared with wild-type mice but improved from Sppl2a^tm1Basc homozygotes

hematopoietic system

decreased B cell number ( J:194664 )

• compared with wild-type mice but improved from Sppl2a^tm1Basc homozygotes in the spleen and lymph nodes

Allelic Composition	Sppl2a^tm1Basc/Sppl2a^tm1Basc Sppl2b^{Gt(Ayu21-T160)Imeg}/Sppl2b^{Gt(Ayu21-T160)Imeg}
Genetic Background	B6N.Cg-Sppl2a^tm1Basc Sppl2b^{Gt(Ayu21-T160)Imeg}

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sppl2a^tm1Basc mutation (0 available); any Sppl2a mutation (47 available)
Sppl2b^{Gt(Ayu21-T160)Imeg} mutation (0 available); any Sppl2b mutation (29 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

decreased B cell number ( J:213519 )

• significant reduction of total B cells in the spleen, lymph nodes, and peritoneal cavity

• B cell numbers similar to mice homozygous only for Sppl2a^tm1Basc

abnormal mature B cell morphology ( J:213519 )

• numerous endosomal vesicles as is seen in mice homozygous only for Sppl2a^tm1Basc

decreased immunoglobulin level ( J:213519 )

• levels decreased to near limit of detectability as seen in mice homozygous only for Sppl2a^tm1Basc

immune system

decreased B cell number ( J:213519 )

• significant reduction of total B cells in the spleen, lymph nodes, and peritoneal cavity

• B cell numbers similar to mice homozygous only for Sppl2a^tm1Basc

abnormal mature B cell morphology ( J:213519 )

• numerous endosomal vesicles as is seen in mice homozygous only for Sppl2a^tm1Basc

decreased immunoglobulin level ( J:213519 )

• levels decreased to near limit of detectability as seen in mice homozygous only for Sppl2a^tm1Basc

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