Phenotypes associated with this allele

• Allele Symbol: Pros1^tm1.2Grl
• Allele Name: targeted mutation 1.2, Greg Lemke
• Allele ID: MGI:5499100
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pros1^tm1.2Grl/Pros1^tm1.2Grl	involves: 129S1/SvImJ * FVB/N	MGI:5499116
ht2	Pros1^tm1.2Grl/Pros1^+	involves: 129S1/SvImJ * FVB/N	MGI:5499115

Genotype
MGI:5499116

hm1

• Allelic Composition: Pros1^tm1.2Grl/Pros1^tm1.2Grl
• Genetic Background: involves: 129S1/SvImJ * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:198769 )

• mice die between E15.5 and E17.5 from massive coagulopathy and associated hemorrhages

cardiovascular system

abnormal blood vessel morphology ( J:198769 )

• poorly formed microvessels in the spinal cord at E15.5

• enlarged spinal cord vessels at E15.5

abnormal brain vasculature morphology ( J:198769 )

• damaged at E13.5 to E15.5

• disperse and disaggregated vessel structure in brain microvasculature at E13.5

abnormal vitelline vasculature morphology ( J:198769 )

• defective vessel development, integrity and function

• no blood-filled vessels are observed in the yolk sac at E14.5 likely due to occlusion

• reduced branch frequency and absence of normal hierarchical branching morphology

vascular smooth muscle hypoplasia ( J:198769 )

• at E13.5

blood vessel congestion ( J:198769 )

• principal blood vessels are not visible at E15.5 consistent with thrombic occlusion

• likely occlusion of yolk sac vasculature

brain vascular congestion ( J:198769 )

• large, perfusion-resistant intravascular blood clots in coronal sections of the brain from E13.5 to E15.5

hemorrhage ( J:198769 )

• mice die between E15.5 and E17.5 from massive coagulopathy and associated hemorrhages

• fulminating hemorrhages throughout the body at mid- to late-gestation

• extravascular hemorrhagic blood in the brain at E13.5 to E15.5

intracranial hemorrhage ( J:198769 )

• pools of blood penetrate into the brain parenchyma from E13.5 to E15.5

increased vascular permeability ( J:198769 )

• blood leakage from interrupted endothelial lining of thin-walled vessels

nervous system

abnormal brain vasculature morphology ( J:198769 )

• damaged at E13.5 to E15.5

• disperse and disaggregated vessel structure in brain microvasculature at E13.5

brain vascular congestion ( J:198769 )

• large, perfusion-resistant intravascular blood clots in coronal sections of the brain from E13.5 to E15.5

intracranial hemorrhage ( J:198769 )

• pools of blood penetrate into the brain parenchyma from E13.5 to E15.5

enlarged brain ventricles ( J:198769 )

• at E13.5 to E15.5

abnormal neocortex morphology ( J:198769 )

• thinning of the developing neocortical laminae at E13.5 to E15.5

embryo

abnormal vitelline vasculature morphology ( J:198769 )

• defective vessel development, integrity and function

• no blood-filled vessels are observed in the yolk sac at E14.5 likely due to occlusion

• reduced branch frequency and absence of normal hierarchical branching morphology

homeostasis/metabolism

abnormal blood coagulation ( J:198769 )

• mice die between E15.5 and E17.5 from massive coagulopathy and associated hemorrhages

abnormal thrombosis ( J:198769 )

• large fibrin blood clots throughout the body (body wall, spinal cord, vascular plexus, lung and liver) brain at mid- to late-gestation

muscle

vascular smooth muscle hypoplasia ( J:198769 )

• at E13.5

Genotype
MGI:5499115

ht2

• Allelic Composition: Pros1^tm1.2Grl/Pros1⁺
• Genetic Background: involves: 129S1/SvImJ * FVB/N

mortality/aging

prenatal lethality, incomplete penetrance ( J:198769 )

• fewer than expected mice are present in the neonatal population

cardiovascular system

abnormal blood vessel morphology ( J:198769 )

increased vascular permeability ( J:198769 )

• in the brain and liver

homeostasis/metabolism

decreased bleeding time ( J:198769 )

• shorter clot time without exogenous activated protein c (aPC)