Phenotypes associated with this allele

• Allele Symbol: Tg(Mrgpra3-GFP/cre)#Xzd
• Allele Name: transgene insertion, Xinzhong Dong
• Allele ID: MGI:5506535
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gt(ROSA)26Sor^tm1(Trpv1,ECFP)Mde/Gt(ROSA)26Sor^+ Trpv1^tm1Jul/Trpv1^tm1Jul Tg(Mrgpra3-GFP/cre)#Xzd/0	involves: 129P2/OlaHsd * 129X1/SvJ	MGI:5506549
cn2	Gt(ROSA)26Sor^tm1(HBEGF)Awai/Gt(ROSA)26Sor^+ Tg(Mrgpra3-GFP/cre)#Xzd/0	involves: C57BL/6	MGI:5506548

Genotype
MGI:5506549

cn1

• Allelic Composition: Gt(ROSA)26Sor^{tm1(Trpv1,ECFP)Mde}/Gt(ROSA)26Sor⁺; Trpv1^tm1Jul/Trpv1^tm1Jul; Tg(Mrgpra3-GFP/cre)#Xzd/0
• Genetic Background: involves: 129P2/OlaHsd * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

hypoalgesia ( J:197482 )

• cheek injection of capsaicin does not evoke pain behavior (forepaw facial wiping), in contrast to robust facial wiping observed in treated wild-type

increased thermal nociceptive threshold ( J:197482 )

• mice have impaired nociceptive responses, showing increased latencies in tail-immersion and hot plate paradigms

integument

increased pruritus ( J:197482 )

• robust itch behavior is observed after cheek injection of capsaicin compared to little hindpaw scratching seen in treated wild-type

Genotype
MGI:5506548

cn2

• Allelic Composition: Gt(ROSA)26Sor^{tm1(HBEGF)Awai}/Gt(ROSA)26Sor⁺; Tg(Mrgpra3-GFP/cre)#Xzd/0
• Genetic Background: involves: C57BL/6

behavior/neurological

behavior/neurological phenotype ( J:197482 )

N • responses to acute noxious heat, cold, mechanical and chemical stimulation after diphtheria toxin (DTX) treatment are similar to control littermates

N • normal motor function is observed following DTX treatment

N • injection of capsaicin to the cheek induces normal pain-invoked facial wiping behavior in DTX-treated animals comparable to controls

N • cheek injection of beta-alanine induces robust scratching response in DTX-treated mice similar to the behavior in treated controls

nervous system

decreased sensory neuron number ( J:197482 )

integument

decreased pruritus ( J:197482 )

• scratching behavior is reduced relative to controls in DTX-treated mice after injection of alpha-methyl-serotonin or endothelin-1

• in models of dry skin pruritus and allergic chronic itch, DTX-treated mice show reduced scratching behavior compared to controls

• injection of chloroquine into cheeks of DTX-treated mice abolishes the hindpaw cheek-scratching elicited in DTX-treated controls

• hindpaw scratching induced by histamine administration to the cheek is reduced in DTX-treated mice relative to treated controls

• subcutaneous injection of histamine into the napes of DTX-treated mice results in a reduced scratching behavior compared to DTX-treated controls

• site-directed scratching is abolished in DTX-treated mutants following injection of either BAN8-22 or SLIGRL-NH2 (pruritogens that activate Mrgprx1)

• after subcutaneous cloroquine injection into the necks of DTX-treated mice, site-directed scratching is significantly reduced compared to DTX- and cholorquine-treated control littermates