Phenotypes associated with this allele

• Allele Symbol: Sarm1^tm1.1Diam
• Allele Name: targeted mutation 1.1, Michael S Diamond
• Allele ID: MGI:5507721
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Sarm1^tm1.1Diam/Sarm1^tm1.1Diam	involves: 129X1/SvJ * C57BL/6	MGI:5507810

Genotype
MGI:5507810

hm1

• Allelic Composition: Sarm1^tm1.1Diam/Sarm1^tm1.1Diam
• Genetic Background: involves: 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

increased susceptibility to Flaviviridae infection induced morbidity/mortality ( J:200140 )

• 36% survival as compared to 63% survival for controls after infection with WNV

immune system

immune system phenotype ( J:196200 , J:200140 )

N • La Crosse virus (LACV) replication is normal (J:196200)

N • no difference in vial burden as compared to controls in spleen, brain, or spinal cord after infection with 10² PFU of West Nile Virus (WNV) (J:200140)

N • no difference in virus specific IgG or IgM at 6, 8, and 10 days after infection (J:200140)

N • no difference in CD8+ T cells in the brain (J:200140)

decreased microglial cell activation ( J:200140 )

• fewer activated microglia, particularly in brainstem and cerebral cortex but not in cerebellum after WNV infection

• macrophage and resting microglia numbers are unchanged

abnormal circulating tumor necrosis factor level ( J:200140 )

• 10 fold lower levels of TNF alpha in brain homogenates relative to controls afer WNV infection

increased susceptibility to Flaviviridae infection ( J:200140 )

• increased vulnerability to infection with 10² PFU of West Nile Virus (WNV)

• elevated WNV RNA in the brainstem but not in the cortex

increased susceptibility to Flaviviridae infection induced morbidity/mortality ( J:200140 )

• 36% survival as compared to 63% survival for controls after infection with WNV

hematopoietic system

hematopoietic system phenotype ( J:200140 )

N • normal hematopoietc cell development after WNV infection

N • typical B and T cell maturation profiles

decreased microglial cell activation ( J:200140 )

• fewer activated microglia, particularly in brainstem and cerebral cortex but not in cerebellum after WNV infection

• macrophage and resting microglia numbers are unchanged

homeostasis/metabolism

abnormal circulating tumor necrosis factor level ( J:200140 )

• 10 fold lower levels of TNF alpha in brain homogenates relative to controls afer WNV infection

nervous system

abnormal nervous system physiology ( J:196200 )

• 100% of controls infected with 10³ PFU of LACV develop neurological disease by 3 weeks whereas homozygous mutant mice develop disease at a significantly lower level

• levels of virus RNA are similar to control levels

• no mitochondrial damage, unlike control mice

decreased neuron apoptosis ( J:196200 )

• apoptosis is reduced in neurons from homozygotes infected with La Crosse virus, LACV, relative to controls (in culture)

• delayed neuronal death

increased neuron apoptosis ( J:200140 )

• increased apoptosis in the brainstem 8-10 days after infection with WNV but not in the cortex, hippocampus, or cerebellum

decreased microglial cell activation ( J:200140 )

• fewer activated microglia, particularly in brainstem and cerebral cortex but not in cerebellum after WNV infection

• macrophage and resting microglia numbers are unchanged

cellular

decreased neuron apoptosis ( J:196200 )

• apoptosis is reduced in neurons from homozygotes infected with La Crosse virus, LACV, relative to controls (in culture)

• delayed neuronal death

increased neuron apoptosis ( J:200140 )

• increased apoptosis in the brainstem 8-10 days after infection with WNV but not in the cortex, hippocampus, or cerebellum