Analysis Tools
immune system
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• thymocytes in immature mutants exhibit a delayed transition of double positive cells into the single positive cells
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• mutants exhibit higher numbers of CD4+CD8+ double positive cells in the thymus at 3-4 weeks of age, but not in adults
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• mutants exhibit lower numbers of CD4+ single positive cells in the blood at 3-4 weeks of age, but not in adults
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• mutants exhibit lower numbers of CD8+ single positive cells in the thymus and blood at 3-4 weeks of age, but not in adults
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• mutants exhibit reduced activation of T cells in a model of contact hypersensitivity
• 24 hours after FITC-sensitization (abdominal hapten application), CD8+ T cells in inguinal lymph nodes exhibit reduced activation
• 5 days after FITC sensitization and challenge with FITC to induce contact hypersensitivity, population of activated CD8+ T cells in the draining lymph nodes is diminished
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• thymocytes of 3 week old mutants exhibit decreased proliferation and reduced maturation
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• mutants exhibit reduced production of inflammatory cytokines at the site of inflammation following FITC challenge, with the highest reduction in lymphotactin, IL-12, and I-TAC
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• mutants exhibit a reduced cutaneous inflammatory reaction to contact hypersensitivity
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• mutants exhibit impaired T cell-mediated immune reaction in a model of contact hypersensitivity, characterized by abrogated ear swelling and thickening of the epidermis, reduced influx of inflammatory cells, low proliferation of keratinocytes, and reduced number of activated CD8+ T cells in draining lymph nodes after cutaneous sensitization and challenge by FITC painting on the ear
• although 72 hours after FITC challenge, mutants do not show any inflammatory signs in the ears, CD8+ T cells in the draining lymph nodes show an increase in CD62L+ cells and a reduction in CD25+ cells
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hematopoietic system
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• thymocytes in immature mutants exhibit a delayed transition of double positive cells into the single positive cells
|
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• mutants exhibit higher numbers of CD4+CD8+ double positive cells in the thymus at 3-4 weeks of age, but not in adults
|
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• mutants exhibit lower numbers of CD4+ single positive cells in the blood at 3-4 weeks of age, but not in adults
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• mutants exhibit lower numbers of CD8+ single positive cells in the thymus and blood at 3-4 weeks of age, but not in adults
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|
• mutants exhibit reduced activation of T cells in a model of contact hypersensitivity
• 24 hours after FITC-sensitization (abdominal hapten application), CD8+ T cells in inguinal lymph nodes exhibit reduced activation
• 5 days after FITC sensitization and challenge with FITC to induce contact hypersensitivity, population of activated CD8+ T cells in the draining lymph nodes is diminished
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• thymocytes of 3 week old mutants exhibit decreased proliferation and reduced maturation
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homeostasis/metabolism
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• mutants exhibit reduced production of inflammatory cytokines at the site of inflammation following FITC challenge, with the highest reduction in lymphotactin, IL-12, and I-TAC
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cellular
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• thymocytes of 3 week old mutants exhibit decreased proliferation and reduced maturation
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