Phenotypes associated with this allele

• Allele Symbol: Wdr62^{tm3a(EUCOMM)Hmgu}
• Allele Name: targeted mutation 3a, Helmholtz Zentrum Muenchen GmbH
• Allele ID: MGI:5511671
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Wdr62^tm3a(EUCOMM)Hmgu/Wdr62^tm3a(EUCOMM)Hmgu	C57BL/6-Wdr62^tm3a(EUCOMM)Hmgu	MGI:6758665

Genotype
MGI:6758665

hm1

• Allelic Composition: Wdr62^{tm3a(EUCOMM)Hmgu}/Wdr62^{tm3a(EUCOMM)Hmgu}
• Genetic Background: C57BL/6-Wdr62^{tm3a(EUCOMM)Hmgu}

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

absent oocytes ( J:306320 )

♀ • no Ddx4+ germ cells in ovaries at age 8 weeks

oligozoospermia ( J:306320 )

♂ • fewer spermatozoa-filled tubules in caput and caudal epididymis

teratozoospermia ( J:306320 )

♂ • misshapen sperm heads

♂ • normal axoneme and flagellum morphology

abnormal sperm head morphology ( J:306320 )

♂ • misshapen sperm heads

abnormal manchette disassembly ( J:306320 )

♂ • failure in manchette disassembly during spermiogenesis steps 13-14

abnormal male meiosis ( J:306320 )

♂ • meiosis initiation delay at ages P28 and adult

♂ • G2/M defect at ages P28 and adult

♂ • fewer spermatocytes in anaphase at ages P28 and adult

♂ • mitotic spindle misalignment in adult metaphase spermatocytes

♂ • under-duplication of centrioles in adult pachytene and diplotene spermatocytes with 75% containing 0-3 centrioles instead of 4

♂ • normal prophase 1 at ages P28 and adult

decreased sperm progressive motility ( J:306320 )

♂ • 50% reduction

asthenozoospermia ( J:306320 )

♂

abnormal seminiferous tubule morphology ( J:306320 )

♂ • 2.4x increase in percentage of stage XII tubules at age 7 months

♂ • loss of round spermatids (61% reduction at stage III, 73% at stage VII) and elongated spermatids (71% at stage III, 82% at stage VII and 79% at stage XII)

♂ • normal number of primary spermatocytes and spermatocytes in metaphase at stage III, VII and XII seminiferous tubules at age 7 months

small testis ( J:306320 )

♂ • at ages P28 and 7 months

decreased testis weight ( J:306320 )

♂ • at ages P28 and 7 months

female infertility ( J:306320 )

♀ • no offspring from matings of mutant females with wild-type or mutant males

male infertility ( J:306320 )

♂ • no offspring from matings of mutant males with wild-type or mutant females

endocrine/exocrine glands

abnormal seminiferous tubule morphology ( J:306320 )

♂ • 2.4x increase in percentage of stage XII tubules at age 7 months

♂ • loss of round spermatids (61% reduction at stage III, 73% at stage VII) and elongated spermatids (71% at stage III, 82% at stage VII and 79% at stage XII)

♂ • normal number of primary spermatocytes and spermatocytes in metaphase at stage III, VII and XII seminiferous tubules at age 7 months

small testis ( J:306320 )

♂ • at ages P28 and 7 months

decreased testis weight ( J:306320 )

♂ • at ages P28 and 7 months

cellular

absent oocytes ( J:306320 )

♀ • no Ddx4+ germ cells in ovaries at age 8 weeks

oligozoospermia ( J:306320 )

♂ • fewer spermatozoa-filled tubules in caput and caudal epididymis

teratozoospermia ( J:306320 )

♂ • misshapen sperm heads

♂ • normal axoneme and flagellum morphology

abnormal sperm head morphology ( J:306320 )

♂ • misshapen sperm heads

abnormal manchette disassembly ( J:306320 )

♂ • failure in manchette disassembly during spermiogenesis steps 13-14

abnormal male meiosis ( J:306320 )

♂ • meiosis initiation delay at ages P28 and adult

♂ • G2/M defect at ages P28 and adult

♂ • fewer spermatocytes in anaphase at ages P28 and adult

♂ • mitotic spindle misalignment in adult metaphase spermatocytes

♂ • under-duplication of centrioles in adult pachytene and diplotene spermatocytes with 75% containing 0-3 centrioles instead of 4

♂ • normal prophase 1 at ages P28 and adult

decreased sperm progressive motility ( J:306320 )

♂ • 50% reduction

asthenozoospermia ( J:306320 )

♂

nervous system

abnormal cortical ventricular zone morphology ( J:306320 )

• decreased number of Pax6+ apical progenitors in E14.5 embryos

abnormal neocortex morphology ( J:306320 )

• 10% increase in differentiating cells in E16.5 embryos

• normal apoptosis level in E14.5 embryos

thin cerebral cortex ( J:306320 )

• reduced thickness of upper layers II-IV in newborns (age P0)

• reduced upper layers II-IV to lower layers V-VI neuron count in newborns

growth/size/body

growth/size/body region phenotype ( J:306320 )

N • normal growth in first 12 weeks