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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Ewsr1tm1c(EUCOMM)Wtsi
targeted mutation 1c, Wellcome Trust Sanger Institute
MGI:5513773
Summary 1 genotype
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Ewsr1tm1c(EUCOMM)Wtsi/Ewsr1tm1d(EUCOMM)Wtsi
Tg(Stra8-icre)1Reb/0 		involves: C57BL/6J MGI:6830505


Genotype
MGI:6830505
cn1
Allelic
Composition		 Ewsr1tm1c(EUCOMM)Wtsi/Ewsr1tm1d(EUCOMM)Wtsi
Tg(Stra8-icre)1Reb/0
Genetic
Background		 involves: C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ewsr1tm1c(EUCOMM)Wtsi mutation (1 available); any Ewsr1 mutation (93 available)
Ewsr1tm1d(EUCOMM)Wtsi mutation (0 available); any Ewsr1 mutation (93 available)
Tg(Stra8-icre)1Reb mutation (2 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
abnormal meiosis ( J:314468 )
• In spermatocytes the number of double stranded breaks, indicated by DMC1 foci, is normal in early leptotene and zygotene but double stranded repair is delayed, PRDM-dependent hotspots differ somewhat from controls, and there are increased unsynapsed chromosomes
arrest of male meiosis ( J:314468 )
• meiosis is blocked at pachynema/diplonema, with normal proportions of leptotene and zygotene spermatocytes, no spermatocytes are found in seminiferous tubules beyond prophase 1, and there is increased apoptotic germ cells in testes
abnormal DNA methylation during gametogenesis ( J:314468 )
• spermatocytes show decreased PRDM9-dependent H3K4 and H3K36 trimethylation: decreased H3K4me3 in leptonema and zygonema and slightly decreased H3K36me3 in leptonema and zygonema but not diplonema; H3K4me3 ChIP-seq from spermatocytes identified fewer than normal peaks, with approximately 38.7% of hotspot peaks missing, in particular those with low H3K4 trimethylation in controls; reduced levels of H3K36 trimethylation, particularly in intergenic region hotspots

reproductive system
abnormal meiosis ( J:314468 )
• In spermatocytes the number of double stranded breaks, indicated by DMC1 foci, is normal in early leptotene and zygotene but double stranded repair is delayed, PRDM-dependent hotspots differ somewhat from controls, and there are increased unsynapsed chromosomes
abnormal DNA methylation during gametogenesis ( J:314468 )
• spermatocytes show decreased PRDM9-dependent H3K4 and H3K36 trimethylation: decreased H3K4me3 in leptonema and zygonema and slightly decreased H3K36me3 in leptonema and zygonema but not diplonema; H3K4me3 ChIP-seq from spermatocytes identified fewer than normal peaks, with approximately 38.7% of hotspot peaks missing, in particular those with low H3K4 trimethylation in controls; reduced levels of H3K36 trimethylation, particularly in intergenic region hotspots
decreased testis weight ( J:314468 )
• decreased testes weight in adults but not at 14 or 18 days of age
arrest of male meiosis ( J:314468 )
• meiosis is blocked at pachynema/diplonema, with normal proportions of leptotene and zygotene spermatocytes, no spermatocytes are found in seminiferous tubules beyond prophase 1, and there is increased apoptotic germ cells in testes

endocrine/exocrine glands
decreased testis weight ( J:314468 )
• decreased testes weight in adults but not at 14 or 18 days of age




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
11/19/2024
MGI 6.24 		The Jackson Laboratory