Phenotypes associated with this allele

• Allele Symbol: Ptpn22^tm1.1Draw
• Allele Name: targeted mutation 1.1, David Rawlings
• Allele ID: MGI:5516594
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ptpn22^tm1.1Draw/Ptpn22^tm1.1Draw	involves: 129 * C57BL/6	MGI:5517354
ht2	Ptpn22^tm1.1Draw/Ptpn22^+	involves: 129 * C57BL/6	MGI:5517355

Genotype
MGI:5517354

hm1

• Allelic Composition: Ptpn22^tm1.1Draw/Ptpn22^tm1.1Draw
• Genetic Background: involves: 129 * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Spectrum of lesions in aged Ptpn22^tm1.1Draw/Ptpn22^tm1.1Draw mice

mortality/aging

premature death ( J:201448 )

• modest

immune system

immune system phenotype ( J:201448 )

N • regulatory T cell suppressive activity is normal

abnormal thymus morphology ( J:201448 )

• infiltration with B cells

thymus hyperplasia ( J:201448 )

• in aged mice

enlarged spleen ( J:201448 )

• at 6 months

spleen hyperplasia ( J:201448 )

• at 6 months

abnormal B cell morphology ( J:201448 )

abnormal B cell differentiation ( J:201448 )

• altered B cell repertoire selection and enrichment of self-reactive B cells

• however, B1 B cells and peripheral blood B cell numbers are normal

decreased mature B cell number ( J:201448 )

• of mature recirculating B cells in aged mice

decreased follicular B cell number ( J:201448 )

• in aged mice

increased B cell number ( J:201448 )

• B220+CD19+ B cells in the thymus of aged mice

• of age-dependent B cells

• in IgM-IgD- isotype switched B cells in the spleen

increased transitional stage T1 B cell number ( J:201448 )

• in the spleen in aged mice

increased germinal center B cell number ( J:201448 )

• in aged mice

abnormal T cell morphology ( J:201448 )

abnormal T cell differentiation ( J:201448 )

• enhanced thymic positive and negative selection

• however, regulatory T cell development is normal

increased double-positive T cell number ( J:201448 )

increased effector memory CD4-positive, alpha-beta T cell number ( J:201448 )

• slight in the spleen and lymph nodes of young mice

• in aged mice

increased effector memory CD8-positive, alpha-beta T cell number ( J:201448 )

• in aged mice

increased regulatory T cell number ( J:201448 )

• in aged mice

increased spleen germinal center size ( J:201448 )

• in aged mice

abnormal B cell physiology ( J:201448 )

decreased B cell apoptosis ( J:201448 )

• of T1 B cells

abnormal B cell activation ( J:201448 )

• increased mature B cell activation

increased B cell proliferation ( J:201448 )

• in response to anti-IgM or anti-IgM plus anti-CD40

• however, response to LPS and CpG is normal

increased IgE level ( J:201448 )

• in untreated mice

increased IgG1 level ( J:201448 )

• in untreated mice

• in response to NP-CGG

increased IgG3 level ( J:201448 )

• in response to TNP-Ficoll

increased IgM level ( J:201448 )

• in response to TNP-Ficoll or NP-CGG

abnormal T cell physiology ( J:201448 )

• increased calcium signaling in naive splenic CD4+ and CD8+ T cells, double positive thymocytes and in vitro-generated effector T cells

increased T cell proliferation ( J:201448 )

• of naive CD4+ T cells in response to all stimuli

• of memory/effector T cells in response to anti-CD3 stimulation

increased interleukin-2 secretion ( J:201448 )

• from stimulated effector T cells

autoimmune response ( J:201448 )

• autoimmune lesions in the glomerulus, lungs and salivary glands with intermittent involvement of the heart, common bile and pancreatic ducts, intestine and adjacent mesentery

increased anti-insulin autoantibody level ( J:201448 )

• in aged mice

increased anti-double stranded DNA antibody level ( J:201448 )

increased inflammatory response ( J:201448 )

• lymphoid infiltrates at 6 months

• autoimmune lesions in the glomerulus, lungs and salivary glands with intermittent involvement of the heart, common bile and pancreatic ducts, intestine and adjacent mesentery

glomerulonephritis ( J:201448 )

• glomerular lesions with cellularity and mesangial matrix, intermittent mesangiolysis or ischemic glomeruli and infiltration of Mac-2+ cells at 10 months

homeostasis/metabolism

increased physiological sensitivity to xenobiotic ( J:201448 )

• mice treated with STZ exhibit increased incidence of type1 diabetes compared with wild-type mice

renal/urinary system

glomerulonephritis ( J:201448 )

• glomerular lesions with cellularity and mesangial matrix, intermittent mesangiolysis or ischemic glomeruli and infiltration of Mac-2+ cells at 10 months

respiratory system

abnormal lung morphology ( J:201448 )

• lesions in the lungs

digestive/alimentary system

abnormal salivary gland morphology ( J:201448 )

• lesions in salivary glands

cardiovascular system

vascular inflammation ( J:201448 )

• at 6 months

cellular

decreased B cell apoptosis ( J:201448 )

• of T1 B cells

increased B cell proliferation ( J:201448 )

• in response to anti-IgM or anti-IgM plus anti-CD40

• however, response to LPS and CpG is normal

increased T cell proliferation ( J:201448 )

• of naive CD4+ T cells in response to all stimuli

• of memory/effector T cells in response to anti-CD3 stimulation

endocrine/exocrine glands

abnormal salivary gland morphology ( J:201448 )

• lesions in salivary glands

abnormal thymus morphology ( J:201448 )

• infiltration with B cells

thymus hyperplasia ( J:201448 )

• in aged mice

hematopoietic system

abnormal thymus morphology ( J:201448 )

• infiltration with B cells

thymus hyperplasia ( J:201448 )

• in aged mice

enlarged spleen ( J:201448 )

• at 6 months

spleen hyperplasia ( J:201448 )

• at 6 months

abnormal B cell morphology ( J:201448 )

abnormal B cell differentiation ( J:201448 )

• altered B cell repertoire selection and enrichment of self-reactive B cells

• however, B1 B cells and peripheral blood B cell numbers are normal

decreased mature B cell number ( J:201448 )

• of mature recirculating B cells in aged mice

decreased follicular B cell number ( J:201448 )

• in aged mice

increased B cell number ( J:201448 )

• B220+CD19+ B cells in the thymus of aged mice

• of age-dependent B cells

• in IgM-IgD- isotype switched B cells in the spleen

increased transitional stage T1 B cell number ( J:201448 )

• in the spleen in aged mice

increased germinal center B cell number ( J:201448 )

• in aged mice

abnormal T cell morphology ( J:201448 )

abnormal T cell differentiation ( J:201448 )

• enhanced thymic positive and negative selection

• however, regulatory T cell development is normal

increased double-positive T cell number ( J:201448 )

increased effector memory CD4-positive, alpha-beta T cell number ( J:201448 )

• slight in the spleen and lymph nodes of young mice

• in aged mice

increased effector memory CD8-positive, alpha-beta T cell number ( J:201448 )

• in aged mice

increased regulatory T cell number ( J:201448 )

• in aged mice

increased spleen germinal center size ( J:201448 )

• in aged mice

abnormal B cell physiology ( J:201448 )

decreased B cell apoptosis ( J:201448 )

• of T1 B cells

abnormal B cell activation ( J:201448 )

• increased mature B cell activation

increased B cell proliferation ( J:201448 )

• in response to anti-IgM or anti-IgM plus anti-CD40

• however, response to LPS and CpG is normal

increased IgE level ( J:201448 )

• in untreated mice

increased IgG1 level ( J:201448 )

• in untreated mice

• in response to NP-CGG

increased IgG3 level ( J:201448 )

• in response to TNP-Ficoll

increased IgM level ( J:201448 )

• in response to TNP-Ficoll or NP-CGG

abnormal T cell physiology ( J:201448 )

• increased calcium signaling in naive splenic CD4+ and CD8+ T cells, double positive thymocytes and in vitro-generated effector T cells

increased T cell proliferation ( J:201448 )

• of naive CD4+ T cells in response to all stimuli

• of memory/effector T cells in response to anti-CD3 stimulation

growth/size/body

enlarged spleen ( J:201448 )

• at 6 months

spleen hyperplasia ( J:201448 )

• at 6 months

Genotype
MGI:5517355

ht2

• Allelic Composition: Ptpn22^tm1.1Draw/Ptpn22⁺
• Genetic Background: involves: 129 * C57BL/6

Increased spleen size in heterozygous and homozygous Ptpn22^tm1.1Draw mice

immune system

abnormal thymus morphology ( J:201448 )

• infiltration with B cells

thymus hyperplasia ( J:201448 )

• in aged mice

enlarged spleen ( J:201448 )

• at 6 months

spleen hyperplasia ( J:201448 )

• at 6 months

abnormal B cell morphology ( J:201448 )

abnormal B cell differentiation ( J:201448 )

• altered B cell repertoire selection and enrichment of self-reactive B cells

abnormal B cell number ( J:201448 )

decreased mature B cell number ( J:201448 )

• of mature recirculating B cells in aged mice

decreased follicular B cell number ( J:201448 )

• in aged mice

increased B cell number ( J:201448 )

• of age-dependent B cells

• in IgM-IgD- isotype switched B cells in the spleen

increased transitional stage T1 B cell number ( J:201448 )

• in the spleen in aged mice

increased germinal center B cell number ( J:201448 )

• in aged mice

abnormal T cell morphology ( J:201448 )

abnormal T cell number ( J:201448 )

increased double-positive T cell number ( J:201448 )

increased CD4-positive, alpha-beta memory T cell number ( J:201448 )

• in aged mice

increased regulatory T cell number ( J:201448 )

• in aged mice

increased spleen germinal center size ( J:201448 )

• in aged mice

abnormal B cell physiology ( J:201448 )

decreased B cell apoptosis ( J:201448 )

• of T1 B cells

increased B cell proliferation ( J:201448 )

• in response to anti-IgM or anti-IgM plus anti-CD40

• however, response to LPS and CpG is normal

increased IgG1 level ( J:201448 )

• in response to NP-CGG

abnormal T cell physiology ( J:201448 )

• increased calcium signaling in naive splenic CD4+ and CD8+ T cells, double positive thymocytes and in vitro-generated effector T cells

increased T cell proliferation ( J:201448 )

• of naive CD4+ T cells in response to all stimuli

• of memory/effector T cells in response to anti-CD3 stimulation

increased interleukin-2 secretion ( J:201448 )

• from stimulated effector T cells

autoimmune response ( J:201448 )

• autoimmune lesions in the lungs, salivary gland, bile and/or pancreatic ducts, heart and gastrointestinal tract

increased anti-double stranded DNA antibody level ( J:201448 )

♀ • in female, but not male, mice

cellular

decreased B cell apoptosis ( J:201448 )

• of T1 B cells

increased B cell proliferation ( J:201448 )

• in response to anti-IgM or anti-IgM plus anti-CD40

• however, response to LPS and CpG is normal

increased T cell proliferation ( J:201448 )

• of naive CD4+ T cells in response to all stimuli

• of memory/effector T cells in response to anti-CD3 stimulation

hematopoietic system

abnormal thymus morphology ( J:201448 )

• infiltration with B cells

thymus hyperplasia ( J:201448 )

• in aged mice

enlarged spleen ( J:201448 )

• at 6 months

spleen hyperplasia ( J:201448 )

• at 6 months

abnormal B cell morphology ( J:201448 )

abnormal B cell differentiation ( J:201448 )

• altered B cell repertoire selection and enrichment of self-reactive B cells

abnormal B cell number ( J:201448 )

decreased mature B cell number ( J:201448 )

• of mature recirculating B cells in aged mice

decreased follicular B cell number ( J:201448 )

• in aged mice

increased B cell number ( J:201448 )

• of age-dependent B cells

• in IgM-IgD- isotype switched B cells in the spleen

increased transitional stage T1 B cell number ( J:201448 )

• in the spleen in aged mice

increased germinal center B cell number ( J:201448 )

• in aged mice

abnormal T cell morphology ( J:201448 )

abnormal T cell number ( J:201448 )

increased double-positive T cell number ( J:201448 )

increased CD4-positive, alpha-beta memory T cell number ( J:201448 )

• in aged mice

increased regulatory T cell number ( J:201448 )

• in aged mice

increased spleen germinal center size ( J:201448 )

• in aged mice

abnormal B cell physiology ( J:201448 )

decreased B cell apoptosis ( J:201448 )

• of T1 B cells

increased B cell proliferation ( J:201448 )

• in response to anti-IgM or anti-IgM plus anti-CD40

• however, response to LPS and CpG is normal

increased IgG1 level ( J:201448 )

• in response to NP-CGG

abnormal T cell physiology ( J:201448 )

• increased calcium signaling in naive splenic CD4+ and CD8+ T cells, double positive thymocytes and in vitro-generated effector T cells

increased T cell proliferation ( J:201448 )

• of naive CD4+ T cells in response to all stimuli

• of memory/effector T cells in response to anti-CD3 stimulation

endocrine/exocrine glands

abnormal thymus morphology ( J:201448 )

• infiltration with B cells

thymus hyperplasia ( J:201448 )

• in aged mice

growth/size/body

enlarged spleen ( J:201448 )

• at 6 months

spleen hyperplasia ( J:201448 )

• at 6 months