Phenotypes associated with this allele

• Allele Symbol: Tg(tetO-Dkk1)1Smr
• Allele Name: transgene insertion 1, Sarah E Millar
• Allele ID: MGI:5532849
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Tg(KRT5-rtTA)#Glk/0 Tg(tetO-Dkk1)1Smr/0	involves: C57BL/6 * FVB/N * SJL	MGI:5538319
cn2	Tg(KRT14-Kremen1)1Smr/0 Tg(KRT5-rtTA)#Glk/0 Tg(tetO-Dkk1)1Smr/0	involves: C57BL/6 * FVB/N * SJL	MGI:5538320
cn3	Tg(tetO-Dkk1)1Smr/0 Tg(KRT14-Kremen1)1Smr/0 Tg(KRT14-cre)#Smr/0	involves: C57BL/6J * SJL/J	MGI:5905990

Genotype
MGI:5538319

cn1

• Allelic Composition: Tg(KRT5-rtTA)#Glk/0; Tg(tetO-Dkk1)1Smr/0
• Genetic Background: involves: C57BL/6 * FVB/N * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

integument

abnormal hair growth ( J:204142 )

• with doxycycline induction starting at P1, mutants completely lack external hair when examined at P100-102

abnormal hair follicle morphology ( J:204142 )

• induction at P4 of P7, reduced proliferation is observed in mutant follicles at P8 or P14

• with doxycycline induction starting at P1, mutant hair follicles show some structural abnormalities by P185

• with doxycycline induction from P4, follicles have slightly increased numbers of apoptotic cells

• with doxycycline treatment starting at P1, no follicle proliferation or hair shaft differentiation is observed; with withdrawal of dox at P100, these are restored

abnormal hair cycle anagen phase ( J:204142 )

• when doxycline induction is performed at P4 or P7 and dorsal skin harvested at P8 or P14, hair follicles in treated skin show cessation of anagen

• with doxycycline treatment starting at P1, hair follicles are arrested in very early anagen; withdrawal of dox at P100 results in follicles spontaneously progressing to full anagen in 15 days

• with induction from P17 (catagen phase), follicles are arrested in early anagen at P30

• with doxycycline induction from P50 and hair plucking at P54, by P57 mutant hair follicles fail to progress through anagen

accelerated hair follicle regression ( J:204142 )

• when doxycline induction is performed at P4 or P7 and dorsal skin harvested at P8 or P14, hair follicles in treated skin show entry into a premature regression phase compared to controls

Genotype
MGI:5538320

cn2

• Allelic Composition: Tg(KRT14-Kremen1)1Smr/0; Tg(KRT5-rtTA)#Glk/0; Tg(tetO-Dkk1)1Smr/0
• Genetic Background: involves: C57BL/6 * FVB/N * SJL

cellular

increased hair follicle apoptosis ( J:204142 )

• with doxycycline induction from P4, follicles have slightly increased numbers of apoptotic cells

integument

increased hair follicle apoptosis ( J:204142 )

• with doxycycline induction from P4, follicles have slightly increased numbers of apoptotic cells

abnormal hair growth ( J:204142 )

• with doxycline induction from P30, mutants have completely lost external hair by P103

abnormal hair cycle anagen phase ( J:204142 )

• when doxycline induction is performed at P4 or P7 and dorsal skin harvested at P8 or P14, hair follicles in treated skin show cessation of anagen; effect is enhanced in triple mutants compared to double mutants by coexpression of Dkk1 with Kremen1

• with induction from P17 (catagen phase), follicles are arrested in early anagen at earlier time than double mutants expressing Dkk1 alone

accelerated hair follicle regression ( J:204142 )

• when doxycline induction is performed at P4 or P7 and dorsal skin harvested at P8 or P14, hair follicles in treated skin show entry into a premature regression phase compared to controls; effect is enhanced in triple mutants compared to double mutants by coexpression of Dkk1 with Kremen1

Genotype
MGI:5905990

cn3

• Allelic Composition: Tg(tetO-Dkk1)1Smr/0; Tg(KRT14-Kremen1)1Smr/0; Tg(KRT14-cre)#Smr/0
• Genetic Background: involves: C57BL/6J * SJL/J

craniofacial

abnormal tongue morphology ( J:242196 )

• flattened surface

digestive/alimentary system

abnormal tongue morphology ( J:242196 )

• flattened surface

endocrine/exocrine glands

abnormal sweat gland morphology ( J:242196 )

• regression of sweat glands when treated with doxycycline at P20

hypohidrosis ( J:242196 )

• when treated with doxycycline at P20

growth/size/body

abnormal tongue morphology ( J:242196 )

• flattened surface

integument

abnormal sweat gland morphology ( J:242196 )

• regression of sweat glands when treated with doxycycline at P20

hypohidrosis ( J:242196 )

• when treated with doxycycline at P20