Phenotypes associated with this allele

• Allele Symbol: Pgap3^tm1.1Ymra
• Allele Name: targeted mutation 1.1, Yoshiko Murakami
• Allele ID: MGI:5538630
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pgap3^tm1.1Ymra/Pgap3^tm1.1Ymra	B6.Cg-Pgap3^tm1.1Ymra	MGI:5538669
hm2	Pgap3^tm1.1Ymra/Pgap3^tm1.1Ymra	involves: 129 * C57BL/6	MGI:5538655

Genotype
MGI:5538669

hm1

• Allelic Composition: Pgap3^tm1.1Ymra/Pgap3^tm1.1Ymra
• Genetic Background: B6.Cg-Pgap3^tm1.1Ymra

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:203443 )

N • mice exhibit normal spleen size and weight, T cell-dependent and T cell-independent B cell responses and regulatory T cells

decreased B cell proliferation ( J:203443 )

• when stimulated with anti-IgM antibodies

• however, proliferation is normal in response to LPS, anti-CD40 antibodies and LPS with IL4

impaired macrophage phagocytosis ( J:203443 )

• reduced efficiency of apoptotic cells engulfment

abnormal T-helper 2 cell differentiation ( J:203443 )

• mice exhibit a tendency of Th2 cell differentiation

increased spleen germinal center number ( J:203443 )

• spontaneous germinal centers formation in 5 of 10 mice

increased anti-double stranded DNA antibody level ( J:203443 )

• in 7 of 26 mice, with highest levels in female mice

increased anti-single stranded DNA antibody level ( J:203443 )

• in 10 of 26 mice, with highest levels in female mice

renal/urinary system

renal glomerulus hypertrophy ( J:203443 )

• in 2 of 4 mice at 13 to 15 months with expansion of matrix between the glomerular basement membrane and capillary endothelium and deposition of immunoglobins

hematopoietic system

decreased B cell proliferation ( J:203443 )

• when stimulated with anti-IgM antibodies

• however, proliferation is normal in response to LPS, anti-CD40 antibodies and LPS with IL4

impaired macrophage phagocytosis ( J:203443 )

• reduced efficiency of apoptotic cells engulfment

abnormal T-helper 2 cell differentiation ( J:203443 )

• mice exhibit a tendency of Th2 cell differentiation

increased spleen germinal center number ( J:203443 )

• spontaneous germinal centers formation in 5 of 10 mice

cellular

decreased B cell proliferation ( J:203443 )

• when stimulated with anti-IgM antibodies

• however, proliferation is normal in response to LPS, anti-CD40 antibodies and LPS with IL4

impaired macrophage phagocytosis ( J:203443 )

• reduced efficiency of apoptotic cells engulfment

Genotype
MGI:5538655

hm2

• Allelic Composition: Pgap3^tm1.1Ymra/Pgap3^tm1.1Ymra
• Genetic Background: involves: 129 * C57BL/6

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:181221 )

• in some mice treated with MOG

reproductive system

reproductive system phenotype ( J:181221 )

N • male and female mice are fertile

immune system

immune system phenotype ( J:181221 )

N • T cell development is normal

decreased T cell proliferation ( J:181221 )

• following Thy-1 cross-linking when TCR signaling is absent

increased T cell proliferation ( J:181221 )

• when TCR signaling is optimal with increasing concentration of anti-Thy1 antibody

• enhanced alloresponse and antigen-primed response of CD4+ T cells

abnormal T cell morphology ( J:181221 )

• glycosylphosphatidylinositol-anchored protein (GPI-AP) without fatty acid remodeling fail to associate with lipid rafts in T cells

abnormal CD4-positive, alpha-beta T cell physiology ( J:181221 )

• enhanced antigen-primed response of CD4+ T cells

increased susceptibility to experimental autoimmune encephalomyelitis ( J:181221 )

• in some mice with severe paralysis and death within 4 weeks after MOG induction

• however, the overall clinical score is normal

limbs/digits/tail

abnormal tail morphology ( J:181221 )

• kinked tails

kinked tail ( J:181221 )

growth/size/body

abnormal head size ( J:181221 )

• short head

postnatal growth retardation ( J:181221 )

behavior/neurological

limb grasping ( J:181221 )

paralysis ( J:181221 )

• in some mice treated with MOG

hematopoietic system

decreased T cell proliferation ( J:181221 )

• following Thy-1 cross-linking when TCR signaling is absent

increased T cell proliferation ( J:181221 )

• when TCR signaling is optimal with increasing concentration of anti-Thy1 antibody

• enhanced alloresponse and antigen-primed response of CD4+ T cells

abnormal T cell morphology ( J:181221 )

• glycosylphosphatidylinositol-anchored protein (GPI-AP) without fatty acid remodeling fail to associate with lipid rafts in T cells

abnormal CD4-positive, alpha-beta T cell physiology ( J:181221 )

• enhanced antigen-primed response of CD4+ T cells

cellular

decreased T cell proliferation ( J:181221 )

• following Thy-1 cross-linking when TCR signaling is absent

increased T cell proliferation ( J:181221 )

• when TCR signaling is optimal with increasing concentration of anti-Thy1 antibody

• enhanced alloresponse and antigen-primed response of CD4+ T cells