Phenotypes associated with this allele

• Allele Symbol: Wfikkn2^tm1.1Sjl
• Allele Name: targeted mutation 1.1, Sejin Lee
• Allele ID: MGI:5546173
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Wfikkn2^tm1.1Sjl/Wfikkn2^tm1.1Sjl	involves: 129 * C57BL/6	MGI:5546331
cx2	Wfikkn1^tm1.1Sjl/Wfikkn1^tm1.1Sjl Wfikkn2^tm1.1Sjl/Wfikkn2^tm1.1Sjl	involves: 129 * C57BL/6	MGI:5546332
cx3	Gdf11^tm1Sjl/Gdf11^tm1Sjl Wfikkn2^tm1.1Sjl/Wfikkn2^tm1.1Sjl	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5546333

Genotype
MGI:5546331

hm1

• Allelic Composition: Wfikkn2^tm1.1Sjl/Wfikkn2^tm1.1Sjl
• Genetic Background: involves: 129 * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Skeletal muscle abnormalities in Wfikkn2^tm1.1Sjl/Wfikkn2^tm1.1Sjl and Wfikkn1^tm1.1Sjl/Wfikkn1^tm1.1Sjl mice

limbs/digits/tail

abnormal limb muscle morphology ( J:201148 )

♂ • the triceps is least effected with a 5% decrease in weight

decreased quadriceps weight ( J:201148 )

♂ • the quadriceps is most the most effected muscle with a 12% in weight

muscle

abnormal limb muscle morphology ( J:201148 )

♂ • the triceps is least effected with a 5% decrease in weight

decreased quadriceps weight ( J:201148 )

♂ • the quadriceps is most the most effected muscle with a 12% in weight

decreased skeletal muscle weight ( J:201148 )

♂ • decrease of 5-12% in muscle weight in males at 10 weeks of age

abnormal skeletal muscle fiber type ratio ( J:201148 )

• seems to be a shift in fiber type from fast glycolytic type IIb fibers to fast oxidative type IIa fibers in the gastrocnemius muscle

impaired skeletal muscle regeneration ( J:201148 )

• impaired regeneration following cardiotoxin induced injury of the gastrocnemius muscle

skeleton

skeleton phenotype ( J:201148 )

N • no skeletal defects

Genotype
MGI:5546332

cx2

• Allelic Composition: Wfikkn1^tm1.1Sjl/Wfikkn1^tm1.1Sjl; Wfikkn2^tm1.1Sjl/Wfikkn2^tm1.1Sjl
• Genetic Background: involves: 129 * C57BL/6

Impaired muscle regeneration in Wfikkn2^tm1.1Sjl/Wfikkn2^tm1.1Sjl, Wfikkn1^tm1.1Sjl/Wfikkn1^tm1.1Sjl, and double mutant mice after cardiotoxin injury

muscle

impaired skeletal muscle regeneration ( J:201148 )

• impaired regeneration following cardiotoxin induced injury of the gastrocnemius muscle

skeleton

abnormal axial skeleton morphology ( J:201148 )

• patterning defects are similar to mice homozygous for Wfikkn1^tm1.1Sjl alone

Genotype
MGI:5546333

cx3

• Allelic Composition: Gdf11^tm1Sjl/Gdf11^tm1Sjl; Wfikkn2^tm1.1Sjl/Wfikkn2^tm1.1Sjl
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Craniofacial defects in Wfikkn2^tm1.1Sjl/Wfikkn2^tm1.1Sjl Gdf11^tm1Sjl/Gdf11^tm1Sjl mice

skeleton

abnormal axial skeleton morphology ( J:201148 )

• anteriorly directed homeotic transformation

abnormal sternocostal joint morphology ( J:201148 )

• have 10 or 11 attached ribs

increased rib number ( J:201148 )

• have 10 or 11 attached ribs

increased thoracic vertebrae number ( J:201148 )

• most have 18 thoracic vertebrae

abnormal cervical vertebrae morphology ( J:201148 )

• rarely the anterior tuberculum is on C7

increased lumbar vertebrae number ( J:201148 )

• have 8, 8/9 or 9 lumbar vertebrae

craniofacial

cleft palate ( J:201148 )

• in all mice

digestive/alimentary system

cleft palate ( J:201148 )

• in all mice

growth/size/body

cleft palate ( J:201148 )

• in all mice