Phenotypes associated with this allele

• Allele Symbol: Gkn2^{tm1.1(KOMP)Vlcg}
• Allele Name: targeted mutation 1.1, Velocigene
• Allele ID: MGI:5548517
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gkn2^tm1.1(KOMP)Vlcg/Gkn2^tm1.1(KOMP)Vlcg	C57BL/6N-Gkn2^tm1.1(KOMP)Vlcg/Ucd	MGI:5797561
hm2	Gkn2^tm1.1(KOMP)Vlcg/Gkn2^tm1.1(KOMP)Vlcg	involves: C57BL/6NTac	MGI:5898088
cx3	Gkn2^tm1.1(KOMP)Vlcg/Gkn2^tm1.1(KOMP)Vlcg Il6st^tm1Ern/Il6st^tm1Ern	involves: 129S1/Sv * C57BL/6NTac	MGI:5898089

Genotype
MGI:5797561

hm1

• Allelic Composition: Gkn2^{tm1.1(KOMP)Vlcg}/Gkn2^{tm1.1(KOMP)Vlcg}
• Genetic Background: C57BL/6N-Gkn2^{tm1.1(KOMP)Vlcg}/Ucd
• Cell Lines: 14915A-B3

Data Sources

IMPC Data for Gkn2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

adipose tissue

decreased total body fat amount ( J:211773 )

♂

IMPC - UCD

cardiovascular system

enlarged heart ( J:211773 )

♂

IMPC - UCD

growth/size/body

enlarged heart ( J:211773 )

♂

IMPC - UCD

increased lean body mass ( J:211773 )

♂

IMPC - UCD

hematopoietic system

decreased mean corpuscular volume ( J:211773 )

♀

IMPC - UCD

homeostasis/metabolism

decreased fasting circulating glucose level ( J:211773 )

♀

IMPC - UCD

limbs/digits/tail

brachydactyly ( J:211773 )

♂

IMPC - UCD

nervous system

decreased brain weight ( J:211773 )

♂

IMPC - UCD

Genotype
MGI:5898088

hm2

• Allelic Composition: Gkn2^{tm1.1(KOMP)Vlcg}/Gkn2^{tm1.1(KOMP)Vlcg}
• Genetic Background: involves: C57BL/6NTac

digestive/alimentary system

abnormal stomach mucosa morphology ( J:234656 )

• abnormalities of the corpus mucosa including focal hypertrophic lesions that are detectable at 6 weeks of age and persist at 12 and 30 weeks of age

• increased incidence of mucus neck cell hyperplasia in the corpus mucosa at 6 and 12 weeks of age

• surface mucus cells show shift from neutral to acidic indicating metaplasia at 6 and 12 weeks of age

• hyperplasia and metaplasia remain elevated at 30 weeks of age

• however, the antral mucosa has normal morphology

gastric gland atrophy ( J:234656 )

• increased incidence in the corpus mucosa at 6 and 12 weeks of age

abnormal digestive system physiology ( J:234656 )

• increased proliferation of gastric corpus mucosal epithelial cell proliferation at 6 and 12 but not 30 weeks of age

stomach inflammation ( J:234656 )

• increased incidence in the corpus mucosa at 6 and 12 weeks of age

• increased severity of H. pylori-induced atrophic gastritis and pervasive mucus metaplasia in the corpus compared to wild-type controls that show mild inflammation with infrequent atrophy and metaplasia

• however, antral inflammation scores after H. pylori infection are similar to wild-type controls

immune system

stomach inflammation ( J:234656 )

• increased incidence in the corpus mucosa at 6 and 12 weeks of age

• increased severity of H. pylori-induced atrophic gastritis and pervasive mucus metaplasia in the corpus compared to wild-type controls that show mild inflammation with infrequent atrophy and metaplasia

• however, antral inflammation scores after H. pylori infection are similar to wild-type controls

abnormal T-helper 1 physiology ( J:234656 )

• antibody and cytokine responses indicate an increased Th1 immune response following H. pylori infection

abnormal cytokine level ( J:234656 )

abnormal chemokine level ( J:234656 )

• increased CXCL1 and CCL4 levels in corpus but not antral gastric tissues

abnormal interleukin level ( J:234656 )

• increased IL16, IL10, and IL1A levels in corpus but not antral gastric tissues

abnormal tumor necrosis factor level ( J:234656 )

• increased TNF level in corpus but not antral gastric tissues

abnormal innate immunity ( J:234656 )

• increased mucosal innate immunity and impaired myeloid-derived suppressor cell responses in mice infected with H. pylori

abnormal susceptibility to bacterial infection ( J:234656 )

• 7 days after H. pylori infection colonization levels are reduced in the gastric mucosa

increased susceptibility to bacterial infection ( J:234656 )

• increased severity of H. pylori-induced atrophic gastritis and pervasive mucus metaplasia in the corpus compared to wild-type controls that show mild inflammation with infrequent atrophy and metaplasia

• however, antral inflammation scores after H. pylori infection are similar to wild-type controls

hematopoietic system

decreased myeloid cell number ( J:234656 )

• decrease in the number of monocytic myeloid-derived suppressor cells in the stomach both before and during H. pylori infection

abnormal T-helper 1 physiology ( J:234656 )

• antibody and cytokine responses indicate an increased Th1 immune response following H. pylori infection

endocrine/exocrine glands

gastric gland atrophy ( J:234656 )

• increased incidence in the corpus mucosa at 6 and 12 weeks of age

cellular

abnormal cell differentiation ( J:234656 )

• impaired basal gastric epithelial differentiation

homeostasis/metabolism

abnormal cytokine level ( J:234656 )

abnormal chemokine level ( J:234656 )

• increased CXCL1 and CCL4 levels in corpus but not antral gastric tissues

abnormal interleukin level ( J:234656 )

• increased IL16, IL10, and IL1A levels in corpus but not antral gastric tissues

abnormal tumor necrosis factor level ( J:234656 )

• increased TNF level in corpus but not antral gastric tissues

Genotype
MGI:5898089

cx3

• Allelic Composition: Gkn2^{tm1.1(KOMP)Vlcg}/Gkn2^{tm1.1(KOMP)Vlcg}; Il6st^tm1Ern/Il6st^tm1Ern
• Genetic Background: involves: 129S1/Sv * C57BL/6NTac

digestive/alimentary system

abnormal stomach mucosa morphology ( J:234656 )

• corpus tumors display poorly differentiated and hyperplastic epithelium histologically

increased stomach mucosa thickness ( J:234656 )

• in the corpus mucosa as a result of tumor formation

• however, antral mucosa thickness is similar to Il6st single mutants

increased stomach tumor incidence ( J:234656 )

• unlike Il6st single mutants which develop primarily antral tumors, double mutants show extensive focal tumorigenesis of the corpus and squamous epithelium overlying the limiting ridge of the corpus/forestomach junction at 12 weeks of age

• increase in both the number of corpus tumor foci and macroscopic corpus tumor area

• however, antral tumor load is similar to Il6st single mutants

neoplasm

increased stomach tumor incidence ( J:234656 )

• unlike Il6st single mutants which develop primarily antral tumors, double mutants show extensive focal tumorigenesis of the corpus and squamous epithelium overlying the limiting ridge of the corpus/forestomach junction at 12 weeks of age

• increase in both the number of corpus tumor foci and macroscopic corpus tumor area

• however, antral tumor load is similar to Il6st single mutants