Phenotypes associated with this allele

• Allele Symbol: Gdnf^{tm1(cre/ERT2)Cos}
• Allele Name: targeted mutation 1, Frank Costantini
• Allele ID: MGI:5553062
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gdnf^tm1(cre/ERT2)Cos/Gdnf^tm1(cre/ERT2)Cos	involves: 129S6/SvEvTac * C57BL/6J	MGI:5553066
cn2	Gdnf^tm1(cre/ERT2)Cos/Gdnf^+ Gt(ROSA)26Sor^tm1(DTA)Jpmb/Gt(ROSA)26Sor^+ Spry1^tm1.1Jdli/Spry1^+	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6J	MGI:5553081
cn3	Gdnf^tm1(cre/ERT2)Cos/Gdnf^+ Gt(ROSA)26Sor^tm1(DTA)Jpmb/Gt(ROSA)26Sor^+	involves: 129S6/SvEvTac * C57BL/6J	MGI:5553068

Genotype
MGI:5553066

hm1

• Allelic Composition: Gdnf^{tm1(cre/ERT2)Cos}/Gdnf^{tm1(cre/ERT2)Cos}
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

renal/urinary system

renal/urinary system phenotype ( J:206853 )

N • mice are viable, fertile, and have normal sized kidneys at birth

Genotype
MGI:5553081

cn2

• Allelic Composition: Gdnf^{tm1(cre/ERT2)Cos}/Gdnf⁺; Gt(ROSA)26Sor^tm1(DTA)Jpmb/Gt(ROSA)26Sor⁺; Spry1^tm1.1Jdli/Spry1⁺
• Genetic Background: involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6J

renal/urinary system

small kidney ( J:206853 )

• with tamoxifen treatment at E12.5, presence of a single copy of Spry1 only marginally improves the hypoplasia observed at E19.5

Genotype
MGI:5553068

cn3

• Allelic Composition: Gdnf^{tm1(cre/ERT2)Cos}/Gdnf⁺; Gt(ROSA)26Sor^tm1(DTA)Jpmb/Gt(ROSA)26Sor⁺
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6J

renal/urinary system

decreased renal glomerulus number ( J:206853 )

• when tamoxifen is administered at E12.5, mutant kidneys have about half the number of glomeruli (48%) at E19.5

• glomerulus number does not recover as well as kidney size with numbers only 57.6% of normal at P50

abnormal kidney development ( J:206853 )

• when tamoxifen is administered at E12.5, fetuses develop severe renal hypoplasia by E14.5, with kidney growth rate reduced relative to wild-type

• nephrogenic zone appears normal at E19.5

small kidney ( J:206853 )

• when tamoxifen is administered at E12.5, at E19.5 kidneys are 55.7% of normal size (based on maximal cross-sectional area); normal gross organization into cortex, medulla and papilla remains

• recovery from tamoxifen treatment at E12.5 is observed postnatally with kidney size reaching 70.4% of control size at P14 and 89.3% at P50

• with tamoxifen treatment at E14.5, resulting kidney morphology is similar at birth with reduced hypoplasia; kidneys are 89% of control size

• when tamoxifen is administered at E9.5, about half the fetuses have severe renal hypoplasia with kidneys about 46.6% of the size of controls

absent kidney ( J:206853 )

• when tamoxifen is administered at E9.5, about half the fetuses display renal agenesis at E18.5