Phenotypes associated with this allele

• Allele Symbol: Actr8^{tm1.1(KOMP)Vlcg}
• Allele Name: targeted mutation 1.1, Velocigene
• Allele ID: MGI:5554924
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Actr8^tm1.1(KOMP)Vlcg/Actr8^tm1.1(KOMP)Vlcg	B6N(Cg)-Actr8^tm1.1(KOMP)Vlcg/J	MGI:5756648
ht2	Actr8^tm1.1(KOMP)Vlcg/Actr8^+	B6N(Cg)-Actr8^tm1.1(KOMP)Vlcg/J	MGI:5756647

Genotype
MGI:5756648

hm1

• Allelic Composition: Actr8^{tm1.1(KOMP)Vlcg}/Actr8^{tm1.1(KOMP)Vlcg}
• Genetic Background: B6N(Cg)-Actr8^{tm1.1(KOMP)Vlcg}/J
• Cell Lines: 19162A-A6

Data Sources

IMPC Data for Actr8

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Actr8^{tm1.1(KOMP)Vlcg}/Actr8^{tm1.1(KOMP)Vlcg} mice exhibit embryonic lethality, with arrest prior to gastrulation and no embryos at E9.5. Embryos form a rudimentary egg-cylinder but no primitive streak of hallmarks of gastrulation are seen at E7.5.

embryo

failure to gastrulate ( J:279207 )

• no morphological hallmarks typical of gastrulating embryos are observed at E7.5

embryonic growth arrest ( J:279207 )

• embryos arrest prior to gastrulation

decreased embryo size ( J:279207 )

• embryos are much smaller than controls at E7.5

decreased egg cylinder size ( J:279207 )

• a rudimentary egg cylinder is observed at E7.5

absent head fold ( J:279207 )

• no head folds are present at E7.5

absent primitive node ( J:279207 )

• no embryonic node is present at E7.5

failure of primitive streak formation ( J:279207 )

• no morphological evidence of primitive streak formation is observed at E7.5

growth/size/body

decreased embryo size ( J:279207 )

• embryos are much smaller than controls at E7.5

mortality/aging

embryonic lethality prior to organogenesis ( J:211773 )

IMPC - JAX

preweaning lethality, complete penetrance ( J:211773 )

IMPC - JAX

embryonic lethality between implantation and placentation, complete penetrance ( J:279207 )

• homozygous embryos are recovered at E7.5 (11% versus expected 25%, n=36) and arrest prior to gastrulation; no homozygous embryos are recovered at E9.5

Genotype
MGI:5756647

ht2

• Allelic Composition: Actr8^{tm1.1(KOMP)Vlcg}/Actr8⁺
• Genetic Background: B6N(Cg)-Actr8^{tm1.1(KOMP)Vlcg}/J
• Cell Lines: 19162A-A6

Data Sources

IMPC Data for Actr8

behavior/neurological

hyperactivity ( J:211773 )

IMPC - JAX

hematopoietic system

decreased erythrocyte cell number ( J:211773 )

♀

IMPC - JAX

decreased hematocrit ( J:211773 )

♀

IMPC - JAX

decreased hemoglobin content ( J:211773 )

♀

IMPC - JAX

homeostasis/metabolism

increased circulating sodium level ( J:211773 )

♂

IMPC - JAX