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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Tg(Nphs1-rtTA*3G)8Jhm
transgene insertion 8, Jeffrey H Miner
MGI:5559153
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cx1
 		Col4a3tm1Jhm/Col4a3tm1Jhm
Tg(Nphs1-rtTA*3G)8Jhm/0
Tg(tetO-COL4A3/Col4a3)#aJhm/0 		involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA MGI:5559180
cx2
 		Hprt1tm1(tetO-EGFP/Rac1*)Shaw/Y
Tg(Nphs1-rtTA*3G)8Jhm/0 		involves: 129S4/SvJae * C57BL/6 MGI:5559184
cx3
 		Tg(Nphs1-rtTA*3G)8Jhm/0
Tg(tetO-GFP,-APOL1*S342G*I384M)#Susz/0 		involves: C57BL/6J * CBA/J * FVB/N MGI:6331339
cx4
 		Tg(Nphs1-rtTA*3G)8Jhm/0
Tg(tetO-GFP,-APOL1*)#Susz/0 		involves: C57BL/6J * CBA/J * FVB/N MGI:6331363
cx5
 		Tg(Nphs1-rtTA*3G)8Jhm/0
Tg(tetO-GFP,-APOL1)#Susz/0 		involves: C57BL/6J * CBA/J * FVB/N MGI:6331386


Genotype
MGI:5559180
cx1
Allelic
Composition		 Col4a3tm1Jhm/Col4a3tm1Jhm
Tg(Nphs1-rtTA*3G)8Jhm/0
Tg(tetO-COL4A3/Col4a3)#aJhm/0
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Col4a3tm1Jhm mutation (1 available); any Col4a3 mutation (60 available)
Tg(Nphs1-rtTA*3G)8Jhm mutation (0 available)
Tg(tetO-COL4A3/Col4a3)#aJhm mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
mortality/aging ( J:207595 )
N
• doxycycline-treated mice survive beyond 32 weeks unlike Col4a3tm1Jhm homozygotes

renal/urinary system
renal/urinary system phenotype ( J:207595 )
N
• doxycycline-treated mice remain nonalbuminuric for months unlike Col4a3tm1Jhm homozygotes
• doxycycline-treated mice do not exhibit glomerulosclerosis or tubular protein cast unlike Col4a3tm1Jhm homozygotes
abnormal podocyte foot process morphology ( J:207595 )
• some doxycycline-treated mice do not maintain podocyte foot process architecture
• however, many doxycycline-treated mice maintain podocyte foot process architecture unlike in Col4a3tm1Jhm homozygotes
abnormal renal glomerulus basement membrane morphology ( J:207595 )
• doxycycline-treated mice exhibit some blebs on the glomerulus basement membrane
increased renal glomerulus basement membrane thickness ( J:207595 )
• doxycycline-treated mice exhibit some of segmental glomerulus basement membrane thickening observed in Col4a3tm1Jhm homozygotes




Genotype
MGI:5559184
cx2
Allelic
Composition		 Hprt1tm1(tetO-EGFP/Rac1*)Shaw/Y
Tg(Nphs1-rtTA*3G)8Jhm/0
Genetic
Background		 involves: 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hprt1tm1(tetO-EGFP/Rac1*)Shaw mutation (0 available); any Hprt1 mutation (1279 available)
Tg(Nphs1-rtTA*3G)8Jhm mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
renal/urinary system
renal/urinary system phenotype ( J:206088 )
N
• glomeruli are unremarkable
increased urine protein level ( J:206088 )
• doxycycline-treated mice exhibit proteinuria that peaks at 4 days and decreases gradually over time unlike control mice

homeostasis/metabolism
increased urine protein level ( J:206088 )
• doxycycline-treated mice exhibit proteinuria that peaks at 4 days and decreases gradually over time unlike control mice




Genotype
MGI:6331339
cx3
Allelic
Composition		 Tg(Nphs1-rtTA*3G)8Jhm/0
Tg(tetO-GFP,-APOL1*S342G*I384M)#Susz/0
Genetic
Background		 involves: C57BL/6J * CBA/J * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
No mouse lines available in IMSR.
See publication links below for author information.
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
homeostasis/metabolism
albuminuria ( J:251848 )
• doxycycline-treated mice exhibit albuminuria, as indicated by higher urinary-albumin-to-creatinine ratio level
• mice treated with doxycycline for 14 days and then taken off it for 7 or 17 days, show halted albuminuria development

renal/urinary system
albuminuria ( J:251848 )
• doxycycline-treated mice exhibit albuminuria, as indicated by higher urinary-albumin-to-creatinine ratio level
• mice treated with doxycycline for 14 days and then taken off it for 7 or 17 days, show halted albuminuria development
abnormal podocyte morphology ( J:251848 )
• marker analysis indicated severe podocyte dedifferentiation in doxycycline-treated mice
podocyte foot process effacement ( J:251848 )
• doxycycline-treated mice exhibit increased foot-process effacement
decreased podocyte number ( J:251848 )
• podocyte number is reduced in doxycycline-treated mice
podocyte vacuoles ( J:251848 )
• increase in number of multivesicular bodies, amphisomes, and autophagosomes in podocytes of doxycycline-treated mice
• the ratio of autophagic vacuoles to autolysosomes is increased in podocytes of doxycycline-treated mice, indicating an increase in autophagic vacuole content
glomerulosclerosis ( J:251848 )
• doxycycline-treated mice show an increase in global and segmental glomerulosclerosis
• by 3 weeks after doxycycline induction, mice develop severe global and segmental sclerosis
abnormal podocyte physiology ( J:251848 )
• podocytes from doxycycline-treated mice show increased inflammatory cell death (pyroptosis)

Mouse Models of Human Disease
 DO ID OMIM ID(s) Ref(s)
kidney disease DOID:557 J:251848




Genotype
MGI:6331363
cx4
Allelic
Composition		 Tg(Nphs1-rtTA*3G)8Jhm/0
Tg(tetO-GFP,-APOL1*)#Susz/0
Genetic
Background		 involves: C57BL/6J * CBA/J * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
No mouse lines available in IMSR.
See publication links below for author information.
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
premature death ( J:251848 )
• increase in mortality rate in doxycycline-treated mice

homeostasis/metabolism
uremia ( J:251848 )
• azotemia in doxycycline-treated mice
increased circulating creatinine level ( J:251848 )
• increase in serum creatinine in doxycycline-treated mice
increased blood urea nitrogen level ( J:251848 )
• increase in serum blood urea nitrogen (BUN) in doxycycline-treated mice
albuminuria ( J:251848 )
• doxycycline-treated mice exhibit albuminuria, as indicated by higher urinary-albumin-to-creatinine ratio level
• mice treated with doxycycline for 14 days and then taken off it for 7 or 17 days, show halted albuminuria development

renal/urinary system
albuminuria ( J:251848 )
• doxycycline-treated mice exhibit albuminuria, as indicated by higher urinary-albumin-to-creatinine ratio level
• mice treated with doxycycline for 14 days and then taken off it for 7 or 17 days, show halted albuminuria development
abnormal podocyte morphology ( J:251848 )
• marker analysis indicated severe podocyte dedifferentiation in doxycycline-treated mice
podocyte foot process effacement ( J:251848 )
• doxycycline-treated mice exhibit increased foot-process effacement
decreased podocyte number ( J:251848 )
• podocyte number is reduced in doxycycline-treated mice
podocyte vacuoles ( J:251848 )
• increase in number of multivesicular bodies, amphisomes, and autophagosomes in podocytes of doxycycline-treated mice
• the ratio of autophagic vacuoles to autolysosomes is increased in podocytes of doxycycline-treated mice, indicating an increase in autophagic vacuole content
glomerulosclerosis ( J:251848 )
• doxycycline-treated mice show an increase in global and segmental glomerulosclerosis
• by 3 weeks after doxycycline induction, mice develop severe global and segmental sclerosis
abnormal podocyte physiology ( J:251848 )
• podocytes from doxycycline-treated mice show increased inflammatory cell death (pyroptosis)

Mouse Models of Human Disease
 DO ID OMIM ID(s) Ref(s)
kidney disease DOID:557 J:251848




Genotype
MGI:6331386
cx5
Allelic
Composition		 Tg(Nphs1-rtTA*3G)8Jhm/0
Tg(tetO-GFP,-APOL1)#Susz/0
Genetic
Background		 involves: C57BL/6J * CBA/J * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
No mouse lines available in IMSR.
See publication links below for author information.
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
homeostasis/metabolism
homeostasis/metabolism phenotype ( J:251848 )
N
• doxycycline-treated mice do not exhibit overt albuminuria

renal/urinary system
renal/urinary system phenotype ( J:251848 )
N
• doxycycline-treated mice do not exhibit overt glomerulosclerosis and do not develop kidney disease




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Send questions and comments to User Support. 		last database update
07/05/2024
MGI 6.24 		The Jackson Laboratory