Phenotypes associated with this allele

• Allele Symbol: Gt(ROSA)26Sor^{tm1(Grem1)Svok}
• Allele Name: targeted mutation 1, Steven Vokes
• Allele ID: MGI:5561086
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gt(ROSA)26Sor^tm1(cre/ERT2)Tyj/Gt(ROSA)26Sor^tm1(Grem1)Svok	involves: 129S1/Sv * 129S4/SvJae	MGI:5588435
cn2	Gt(ROSA)26Sor^tm1(Grem1)Svok/Gt(ROSA)26Sor^+ Shh^tm1(EGFP/cre)Cjt/0	involves: 129S1/Sv	MGI:5588383
cn3	Gt(ROSA)26Sor^tm1(Grem1)Svok/Gt(ROSA)26Sor^+ Tg(Prrx1-cre)1Cjt/0	involves: 129S1/Sv * C57BL/6J * SJL/J	MGI:5588382
cn4	Gt(ROSA)26Sor^tm1(Grem1)Svok/Gt(ROSA)26Sor^tm1(Grem1)Svok Tg(Hoxb6-cre/ERT)1Smac/0	involves: 129S1/Sv * FVB/N	MGI:5588431
cn5	Gt(ROSA)26Sor^tm1(Grem1)Svok/Gt(ROSA)26Sor^+ Tg(Hoxb6-cre/ERT)1Smac/0	involves: 129S1/Sv * FVB/N	MGI:5588434

Genotype
MGI:5588435

cn1

• Allelic Composition: Gt(ROSA)26Sor^{tm1(cre/ERT2)Tyj}/Gt(ROSA)26Sor^{tm1(Grem1)Svok}
• Genetic Background: involves: 129S1/Sv * 129S4/SvJae

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

limbs/digits/tail

abnormal digit morphology ( J:214075 )

• following tamoxifen administration at E9.5, hindlimb digits exhibit nubs (ectopic pieces of cartilage (7/12)

• following tamoxifen administration at E10.5, hindlimb digits exhibit nubs (5/5

• following tamoxifen administration at E9.5 and E10.5, all forelimb digits exhibit nubs

• following tamoxifen administration at E11.5, some forelimb digits exhibit nubs (3/5)

• following tamoxifen administraion, hindlimbs and forelimbs do not form nubs or polydactyly after E11.5

polydactyly ( J:214075 )

• following tamoxifen administration at E9.5, hindlimb digits exhibit polydactyly (5/12)

• following tamoxifen administration, hindlimbs and forelimbs do not form nubs or polydactyly after E11.5

Genotype
MGI:5588383

cn2

• Allelic Composition: Gt(ROSA)26Sor^{tm1(Grem1)Svok}/Gt(ROSA)26Sor⁺; Shh^{tm1(EGFP/cre)Cjt}/0
• Genetic Background: involves: 129S1/Sv

limbs/digits/tail

polyphalangy ( J:214075 )

• hindlimbs from some E18.5 embryos have multiple posterior distal bifurcations (1/4)

• forelimbs from some E18.5 embryos have large nubs in the posterior (3/4)

postaxial polydactyly ( J:214075 )

• hindlimbs from some E18.5 embryos have posterior polydactyly (3/4)

preaxial polydactyly ( J:214075 )

• forelimbs from some E18.5 embryos have preaxial polydactyly (1/4)

skeleton

polyphalangy ( J:214075 )

• hindlimbs from some E18.5 embryos have multiple posterior distal bifurcations (1/4)

• forelimbs from some E18.5 embryos have large nubs in the posterior (3/4)

Genotype
MGI:5588382

cn3

• Allelic Composition: Gt(ROSA)26Sor^{tm1(Grem1)Svok}/Gt(ROSA)26Sor⁺; Tg(Prrx1-cre)1Cjt/0
• Genetic Background: involves: 129S1/Sv * C57BL/6J * SJL/J

limbs/digits/tail

abnormal forelimb bud morphology ( J:214075 )

• forelimbs of most (7/10) E11.5 embryos have shriveled limb buds with small patches of apical ectodermal ridge (AER) as compared to controls

abnormal digit morphology ( J:214075 )

• hindlimb phalanges have delays in ossification that is most obvious at tips

• hindlimb digits (E18.5) are more uniform in length as compared to controls

abnormal hallux morphology ( J:214075 )

• all embryos exhibit a transformation of digit 1 from a shorter digit with 2 phalanges to a longer digit with 3 phalanges

brachydactyly ( J:214075 )

• hindlimb digits (E18.5) are shorter than controls

• shortened digits are reduced proportionally

forelimb oligodactyly ( J:214075 )

• 2 embryos (2/10) have reduced numbers of digits on both forelimbs

polydactyly ( J:214075 )

• 1 embryo (1/10) has polydactyly on both forelimbs

• hindlimb autopods are polydactyl (6-9 digits) with soft tissue syndactyly

syndactyly ( J:214075 )

• hindlimbs are polydactyl (6-9 digits) with soft tissue syndactyly

triphalangia ( J:214075 )

absent forelimb ( J:214075 )

• most E11.5 embryos (7/10) lack forelimbs and have only residual cartilage attached to the scapula

abnormal hindlimb morphology ( J:214075 )

• size of hindlimbs is visibly increased by E11.5

embryo

abnormal forelimb bud morphology ( J:214075 )

• forelimbs of most (7/10) E11.5 embryos have shriveled limb buds with small patches of apical ectodermal ridge (AER) as compared to controls

cellular

decreased apoptosis ( J:214075 )

• hindlimbs have reduced levels of apoptosis in anterior AER and mesoderm beginning at E11.75

• numbers of apoptotic cells in the posterior zone are reduced in limb buds

• normal anterior necrotic zone is absent

increased cell proliferation ( J:214075 )

• hindlimbs have a 20% increase in mitotic index as compared to controls

Genotype
MGI:5588431

cn4

• Allelic Composition: Gt(ROSA)26Sor^{tm1(Grem1)Svok}/Gt(ROSA)26Sor^{tm1(Grem1)Svok}; Tg(Hoxb6-cre/ERT)1Smac/0
• Genetic Background: involves: 129S1/Sv * FVB/N

limbs/digits/tail

abnormal digit morphology ( J:214075 )

• following tamoxifen administration at E11.5, hindlimb digits have nubs (ecotopic pieces of cartilage) (2/2)

• following tamoxifen administration at E10, forelimb digits exhibit nubs (8/8)

• following tamoxifen administration at E10.5 or E11, forelimb digits exhibit nubs (3/3)

• following tamoxifen administration at E11.5, forelimbs develop normally

polyphalangy ( J:214075 )

• following tamoxifen administration at E11, hindlimb digits exhibit multiple distal bifurcations (3/4), but no completely formed extra digits

brachydactyly ( J:214075 )

• following tamoxifen administration at E9.5, forelimbs exhibit digit reduction (3/5)

polydactyly ( J:214075 )

• following tamoxifen administration at E10 and E10.5, hindlimbs have polydactyly

bowed femur ( J:214075 )

• following tamoxifen administration at E10 (5/8) and E10.5(2/3) some embryos exhibit bowed femurs and tibia

absent fibula ( J:214075 )

• following tamoxifen administration at E10 (5/8) and E10.5(2/3) some embryos exhibit reduced or absent fibula

short fibula ( J:214075 )

• following tamoxifen administration at E10 (5/8) and E10.5(2/3) some embryos exhibit reduced or absent fibula

bowed tibia ( J:214075 )

• ollowing tamoxifen administration at E10 (5/8) and E10.5(2/3) some embryos exhibit bowed femurs and tibia

absent hindlimb ( J:214075 )

• following tamoxifen administration at E9.5, hindlimbs are almost absent and contain only a few digit-like elements in 3/5 embryos

skeleton

polyphalangy ( J:214075 )

• following tamoxifen administration at E11, hindlimb digits exhibit multiple distal bifurcations (3/4), but no completely formed extra digits

bowed femur ( J:214075 )

• following tamoxifen administration at E10 (5/8) and E10.5(2/3) some embryos exhibit bowed femurs and tibia

absent fibula ( J:214075 )

• following tamoxifen administration at E10 (5/8) and E10.5(2/3) some embryos exhibit reduced or absent fibula

short fibula ( J:214075 )

• following tamoxifen administration at E10 (5/8) and E10.5(2/3) some embryos exhibit reduced or absent fibula

bowed tibia ( J:214075 )

• ollowing tamoxifen administration at E10 (5/8) and E10.5(2/3) some embryos exhibit bowed femurs and tibia

Genotype
MGI:5588434

cn5

• Allelic Composition: Gt(ROSA)26Sor^{tm1(Grem1)Svok}/Gt(ROSA)26Sor⁺; Tg(Hoxb6-cre/ERT)1Smac/0
• Genetic Background: involves: 129S1/Sv * FVB/N

limbs/digits/tail

abnormal digit morphology ( J:214075 )

• following tamoxifen administration at E10, hindlimb digits (1/1) are nubs (ectopic pieces of cartilage)

• following tamoxifen administration at E11.5, hindlimb digits have nubs (2/2), but nubs are smaller than nubs observed at earlier time points

• following tamoxifen administration at E10, E10.5 and E11, forelimb digits exhibit nubs

• following tamoxifen administration at E11.5, forelimbs develop normally

polyphalangy ( J:214075 )

• following tamoxifen administration at E11, hindlimb digits exhibit distal bifurcation (1/6) or have nubs (5/6)

brachydactyly ( J:214075 )

• following tamoxifen administration at E9.5, forelimb digits exhibit digit reduction (1/3)

polydactyly ( J:214075 )

• following tamoxifen administration at E10.5, hindlimb digits exhibit polydactyly (3/6), exhibit distal bifurcation (1/6) or have nubs (2/6)

absent hindlimb ( J:214075 )

• following tamoxifen administration at E9.5, some embryos have no hindlimbs (1/4)

skeleton

polyphalangy ( J:214075 )

• following tamoxifen administration at E11, hindlimb digits exhibit distal bifurcation (1/6) or have nubs (5/6)