Phenotypes associated with this allele

• Allele Symbol: Tg(Wt1-cre)1Jbeb
• Allele Name: transgene insertion 1, John Burch
• Allele ID: MGI:5562908
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gata4^tm1.1Sad/Gata4^tm1.1Sad Gata6^tm2.1Sad/Gata6^tm2.1Sad Tg(Wt1-cre)1Jbeb/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:5562926
cn2	Gata4^tm1.1Sad/Gata4^+ Gata6^tm2.1Sad/Gata6^tm2.1Sad Tg(Wt1-cre)1Jbeb/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:5562927
cn3	Gata4^tm1.1Sad/Gata4^tm1.1Sad Gata6^tm2.1Sad/Gata6^+ Tg(Wt1-cre)1Jbeb/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:5562928

Genotype
MGI:5562926

cn1

• Allelic Composition: Gata4^tm1.1Sad/Gata4^tm1.1Sad; Gata6^tm2.1Sad/Gata6^tm2.1Sad; Tg(Wt1-cre)1Jbeb/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:206877 )

• normal Mendelian ratios are observed through E14.5, but all mutants are necrotic by E15.5; no living animals are present at weaning

cardiovascular system

abnormal cardiovascular development ( J:206877 )

• at E13.5, hearts display some coronary plexus formation near the base with significantly vessel decreased spreading towards the apex

abnormal heart layer morphology ( J:206877 )

• numbers of sub-epicardial endothelial cells are decreased by <90% at E13.5 compared to wild-type

• numbers of epicardial cells are not different from wild type at E13.5

• 3-fold fewer inner-myocardial endothelial cells are detected in mutant hearts at E13.5

thin myocardium ( J:206877 )

• one third of E13.5 hearts display myocardial thinning (<2 SD from mean of wild-type)

thin ventricular wall ( J:206877 )

• mutants show decreased ventricular thickness compared to wild-type

decreased fetal cardiomyocyte proliferation ( J:206877 )

• at E13.5, myocardial proliferation is decreased compared to wild-type, contribution to the observed myocardial thinning

limbs/digits/tail

polydactyly ( J:206877 )

• hind limb polydactyly is observed in E14.5 embryos

cellular

decreased fetal cardiomyocyte proliferation ( J:206877 )

• at E13.5, myocardial proliferation is decreased compared to wild-type, contribution to the observed myocardial thinning

muscle

thin myocardium ( J:206877 )

• one third of E13.5 hearts display myocardial thinning (<2 SD from mean of wild-type)

decreased fetal cardiomyocyte proliferation ( J:206877 )

• at E13.5, myocardial proliferation is decreased compared to wild-type, contribution to the observed myocardial thinning

Genotype
MGI:5562927

cn2

• Allelic Composition: Gata4^tm1.1Sad/Gata4⁺; Gata6^tm2.1Sad/Gata6^tm2.1Sad; Tg(Wt1-cre)1Jbeb/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

mortality/aging

preweaning lethality, incomplete penetrance ( J:206877 )

• mutants are viable through E15.5, with a small number recovered at weaning

cardiovascular system

abnormal heart layer morphology ( J:206877 )

• a statistically significant decrease in sub-epicardicardial endothelial cells is observed at E13.5

Genotype
MGI:5562928

cn3

• Allelic Composition: Gata4^tm1.1Sad/Gata4^tm1.1Sad; Gata6^tm2.1Sad/Gata6⁺; Tg(Wt1-cre)1Jbeb/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

mortality/aging

preweaning lethality, incomplete penetrance ( J:206877 )

• mutants are viable through E15.5, with a small number recovered at weaning

cardiovascular system

abnormal heart layer morphology ( J:206877 )

• a statistically significant decrease in sub-epicardicardial endothelial cells is observed at E13.5