All Phenotypes Dhfr<orana> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Dhfr^orana/Dhfr^orana	involves: C57BL/6JAnu	MGI:5906027
ht2	Dhfr^orana/Dhfr⁺	involves: C57BL/6JAnu	MGI:5906028

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

lethality during fetal growth through weaning, complete penetrance ( J:236197 )

• mice die soon after E14.5

hematopoietic system

decreased common myeloid progenitor cell number ( J:236197 )

• at E11.5 and E13.5

decreased granulocyte monocyte progenitor cell number ( J:236197 )

• at E11.5

decreased erythroid progenitor cell number ( J:236197 )

• at E11.5 and E13.5

decreased erythrocyte cell number ( J:236197 )

• in the livers at E11.5 and E13.5

increased nucleated erythrocyte cell number ( J:236197 )

growth/size/body

decreased embryo size ( J:236197 )

• at E13.5

decreased fetal size ( J:236197 )

• at E14.5

liver/biliary system

liver hypoplasia ( J:236197 )

• at E11.5 and E13.5

pale liver ( J:236197 )

• slightly at E11.5

homeostasis/metabolism

abnormal folic acid level ( J:236197 )

• less than half of folate in whole embryo at E13.5

• 9-fold lower 5,6,7,8-tetrahydrofolate (THF) in whole embryo at E13.5

• 15-fold higher folic acid levels in whole embryo at E13.5

• reduced total folate, 5-methyl THF and THF concentrations in E13.5 livers

integument

pallor ( J:236197 )

• at E13.5 and E14.5

cellular

abnormal DNA methylation ( J:236197 )

• small increase in average methylation across Kcnq1ot1 CpG island

embryo

decreased embryo size ( J:236197 )

• at E13.5

Allelic Composition	Dhfr^orana/Dhfr⁺
Genetic Background	involves: C57BL/6JAnu

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dhfr^orana mutation (0 available); any Dhfr mutation (18 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

enlarged spleen ( J:236197 )

• following exposure to 5-fluorouracil

abnormal hemopoiesis ( J:236197 )

• in untreated mice and following exposure to 5-fluorouracil

extramedullary hematopoiesis ( J:236197 )

• prolonged following exposure to 5-fluorouracil

decreased granulocyte monocyte progenitor cell number ( J:236197 )

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:236197 )

N	• mice exhibit normal plasma homocysteine levels

abnormal folic acid level ( J:236197 )

• half as much total folate in the bone marrow as in wild-type mice

• however, sub-species levels are normal

cardiovascular system

decreased vasodilation ( J:236197 )

• impaired endothelium-dependent in the aorta

• however, mice exhibit normal relaxation induced by the NO donor DEANO

immune system

enlarged spleen ( J:236197 )

• following exposure to 5-fluorouracil

muscle

decreased vasodilation ( J:236197 )

• impaired endothelium-dependent in the aorta

• however, mice exhibit normal relaxation induced by the NO donor DEANO

growth/size/body

enlarged spleen ( J:236197 )

• following exposure to 5-fluorouracil

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