Phenotypes associated with this allele

• Allele Symbol: Nlgn2^tm1.1Sud
• Allele Name: targeted mutation 1.1, Thomas C Sudhof
• Allele ID: MGI:5567078
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Nlgn2^tm1.1Sud/Nlgn2^tm1.1Sud	involves: C57BL/6NTac	MGI:5567079
cn2	Nlgn2^tm1.1Sud/Nlgn2^tm1.1Sud Tg(Pcp2-cre)2Mpin/?	involves: 129S1/Sv * 129X1/SvJ * C57BL/6NTac	MGI:5754961
cn3	Nlgn2^tm1.1Sud/Nlgn2^tm1.1Sud Nlgn3^tm4.1Sud/Y Tg(Pcp2-cre)2Mpin/?	involves: 129S1/Sv * 129X1/SvJ * C57BL/6NTac	MGI:5754962
cn4	Nlgn1^tm1.1Sud/Nlgn1^tm1.1Sud Nlgn2^tm1.1Sud/Nlgn2^tm1.1Sud Nlgn3^tm4.1Sud/Y Tg(Pcp2-cre)2Mpin/?	involves: 129S1/Sv * 129X1/SvJ * C57BL/6NTac	MGI:5754963

Genotype
MGI:5567079

cn1

• Allelic Composition: Nlgn2^tm1.1Sud/Nlgn2^tm1.1Sud
• Genetic Background: involves: C57BL/6NTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal synapse morphology ( J:229887 )

• GAD65-positive synapse density is decreased 6-7 weeks following injection of adeno-associated virus (AAV) expressing Cre recombinase

• 15% decrease in inhibitory synapse numbers in medial prefrontal cortex 6-7 weeks following AAV-cre

• no major change in passive or active neuron membrane properties are detected

abnormal CNS synaptic transmission ( J:229887 )

• 45% decrease in synaptic transmission in medial prefrontal cortex following AAV-cre

abnormal inhibitory postsynaptic currents ( J:229887 )

• decreased IPSC amplitudes 6-7 weeks following AAV-cre, but no decrease in EPSC amplitudes

• decrease is not detected 2-3 weeks following AAV-cre injection

• E/I ratio is increased 6-7 weeks following AAV-cre injection

abnormal miniature inhibitory postsynaptic currents ( J:229887 )

• amplitude and frequency of mIPSC is decreased 6-7 weeks following AAV-cre

• no changes in mEPSC amplitude or frequency are detected

decreased miniature inhibitory postsynaptic current amplitude ( J:229887 )

• at 6-7 weeks following AAV-cre

decreased miniature inhibitory postsynaptic current frequency ( J:229887 )

• at 6-7 weeks following AAV-cre

behavior/neurological

behavior/neurological phenotype ( J:229887 )

N • no alteration in rotarod performance, open field tests and Y-maze tests as compared to controls

impaired contextual conditioning behavior ( J:229887 )

• decrease in contextual fear conditioning beginning 2-3 weeks following AAV-cre

impaired cued conditioning behavior ( J:229887 )

• decrease in cued fear conditioning beginning 2-3 weeks following AAV-cre

decreased anxiety-related response ( J:229887 )

• mice spend more time in open arms of elevated plus maze beginning 2-3 weeks following AAV-cre

social withdrawal ( J:229887 )

• decrease in social interactions observed 6-7 weeks following AAV-cre

Genotype
MGI:5754961

cn2

• Allelic Composition: Nlgn2^tm1.1Sud/Nlgn2^tm1.1Sud; Tg(Pcp2-cre)2Mpin/?
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6NTac

nervous system

nervous system phenotype ( J:227671 )

N • no change in density, distribution or size of excitatory climbing-fiber synapses or inhibitory basket/stellate cell synapses

increased excitatory postsynaptic current amplitude ( J:227671 )

• 25% increase in climbing fiber synapse EPSC amplitude

abnormal inhibitory postsynaptic currents ( J:227671 )

• 50% reduction in IPSC amplitude

decreased miniature inhibitory postsynaptic current frequency ( J:227671 )

• 67% decrease in spontaneous mIPSC frequency, but no change in amplitude or kinetics

Genotype
MGI:5754962

cn3

• Allelic Composition: Nlgn2^tm1.1Sud/Nlgn2^tm1.1Sud; Nlgn3^tm4.1Sud/Y; Tg(Pcp2-cre)2Mpin/?
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6NTac

nervous system

nervous system phenotype ( J:227671 )

N • no change in parallel fiber synpatic transmission

abnormal synapse morphology ( J:227671 )

• slight decrease in climbing-fiber synapse (vGluT2 staining) cluster density and size

decreased excitatory postsynaptic current amplitude ( J:227671 )

• decrease in climbing-fiber EPSC amplitude, but no change in quantal events

abnormal miniature inhibitory postsynaptic currents ( J:227671 )

• large decrease in frequency (90%) and amplitude (45%) of mIPScs

decreased miniature inhibitory postsynaptic current amplitude ( J:227671 )

• 45% decrease

decreased miniature inhibitory postsynaptic current frequency ( J:227671 )

• 90% decrease

Genotype
MGI:5754963

cn4

• Allelic Composition: Nlgn1^tm1.1Sud/Nlgn1^tm1.1Sud; Nlgn2^tm1.1Sud/Nlgn2^tm1.1Sud; Nlgn3^tm4.1Sud/Y; Tg(Pcp2-cre)2Mpin/?
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6NTac

nervous system

abnormal synaptic bouton morphology ( J:227671 )

• bouton size is reduced

abnormal synapse morphology ( J:227671 )

• 50% decrease in density of climbing fiber synapse on distal Purkinje cell dendrites

• 15% decrease in density of climbing fiber synapse on proximal Purkinje cell dendrites

• inhibitory basket/stellate cell synapses are increased in size, but not number

• 25% decrease in size of climbing fiber synapse on distal and proximal Purkinje cell dendrites

• increase in puncta density as detected by GAD65

• parallel fiber synapse density is not altered

abnormal CNS synaptic transmission ( J:227671 )

• decreased frequency of delayed quantal release events, but not amplitude events

• impaired synaptic transmission at GABAergic synapses

decreased excitatory postsynaptic current amplitude ( J:227671 )

• 40% decrease in amplitude of climbing fiber EPSCs in juvenile and adult mice

• however, no change in kinetics or paired pulse ratio of climbing fiber EPSCs and no alterations in parallel-fiber EPSCs

abnormal inhibitory postsynaptic currents ( J:227671 )

• 80% decrease in IPSC amplitude in Purkinje cells, but no changes in kinetics or paired pulse ratio

abnormal miniature inhibitory postsynaptic currents ( J:227671 )

• 80% decrease in mIPSC frequency and amplitude (45%) in basket/stellate cell synapses

• decrease in decay, but not rise times of mIPSCs

decreased miniature inhibitory postsynaptic current amplitude ( J:227671 )

• 45% decrease in basket/stellate cell synapses

decreased miniature inhibitory postsynaptic current frequency ( J:227671 )

• 80% decrease in basket/stellate cell synapses