Phenotypes associated with this allele

• Allele Symbol: Shox2^{tm1.1(cre)Oki}
• Allele Name: targeted mutation 1.1, Ole Kiehn
• Allele ID: MGI:5567920
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Mapt^tm2Arbr/Mapt^+ Shox2^tm1.1(cre)Oki/Shox2^+ Slc17a6^tm1.1Thna/Slc17a6^tm1.2Edw	involves: 129P2/OlaHsd * 129S/SvEv * C57BL/6J	MGI:5576701
cn2	Gt(ROSA)26Sor^tm39.1(CAG-hop/EYFP)Hze/Gt(ROSA)26Sor^+ Shox2^tm1.1(cre)Oki/Shox2^+	involves: 129S/SvEv * 129S6/SvEvTac * C57BL/6	MGI:5576702
cn3	Shox2^tm1.1(cre)Oki/Shox2^+ Tg(CAG-Bgeo/GFP)21Lbe/0 Vsx2^tm1(DTA)Kash/Vsx2^+	involves: 129S/SvEv * 129X1/SvJ * C57BL/6J	MGI:5576700

Genotype
MGI:5576701

cn1

• Allelic Composition: Mapt^tm2Arbr/Mapt⁺; Shox2^{tm1.1(cre)Oki}/Shox2⁺; Slc17a6^tm1.1Thna/Slc17a6^tm1.2Edw
• Genetic Background: involves: 129P2/OlaHsd * 129S/SvEv * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal nervous system physiology ( J:209344 )

• frequencies of locomotor activity induced in isolated spinal cords increase with increasing NMDA concentrations, but are significantly lower than those observed in control spinal cords

• locomotor burst duration increases but duty cycle is not changed in mutant preparations (increased interburst interval)

• coefficients of variation of main locomotor parameters (cycle period, burst duration, amplitude, duty cycle) are increased compared to controls

• spinal cords from mutants display lower drug-evoked and stimulus-evoked locomotor frequencies

• flexor-extensor coupling is not significantly changed at any locomotor frequency

Genotype
MGI:5576702

cn2

• Allelic Composition: Gt(ROSA)26Sor^{tm39.1(CAG-hop/EYFP)Hze}/Gt(ROSA)26Sor⁺; Shox2^{tm1.1(cre)Oki}/Shox2⁺
• Genetic Background: involves: 129S/SvEv * 129S6/SvEvTac * C57BL/6

nervous system

abnormal nervous system physiology ( J:209344 )

• electrical stimulation of spinal cords to induce locomotor activity is decreased by about 25% when Shox2 interneurons are inactivated by light, which is similar to the phenotype observed when the population of Shox2 interneurons is ablated

Genotype
MGI:5576700

cn3

• Allelic Composition: Shox2^{tm1.1(cre)Oki}/Shox2⁺; Tg(CAG-Bgeo/GFP)21Lbe/0; Vsx2^tm1(DTA)Kash/Vsx2⁺
• Genetic Background: involves: 129S/SvEv * 129X1/SvJ * C57BL/6J

nervous system

abnormal spinal cord grey matter morphology ( J:209344 )

• number of Shox2-expressing V2a interneurons is reduced by 98%; total number of Shox2-expressing interneurons is reduced by >80%

abnormal nervous system physiology ( J:209344 )

• variability in locomotor parameters (cycle period, burst duration, amplitude) that are observed in isolated neonatal spinal cords exposed to low NMDA concentrations is increased in mutants compared to controls; higher NMDA concentrations reduce this variability

• other parameters such as burst frequency and flexor-extensor alternation are not significantly different