Phenotypes associated with this allele

• Allele Symbol: Tg(Ins2-GP)#Psoh
• Allele Name: transgene insertion, Pamela Ohashi
• Allele ID: MGI:5568709
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Tg(Ins2-GP)#Psoh/0 Tg(TcrLCMV)327Sdz/0	involves: C57BL/6 * DBA/2	MGI:5568711
cx2	Tg(Ins1-Cd80)378Psoh/0 Tg(Ins2-GP)#Psoh/0 Tg(TcrLCMV)327Sdz/0	involves: C57BL/6 * DBA/2 * FVB/N	MGI:5569019
cx3	Tg(Ins1-Cd80)378Psoh/0 Tg(Ins2-GP)#Psoh/0	involves: C57BL/6 * FVB/N	MGI:5569021
tg4	Tg(Ins2-GP)#Psoh/0	involves: C57BL/6	MGI:5568710

Genotype
MGI:5568711

cx1

• Allelic Composition: Tg(Ins2-GP)#Psoh/0; Tg(TcrLCMV)327Sdz/0
• Genetic Background: involves: C57BL/6 * DBA/2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

abnormal B cell selection ( J:81285 )

• skewing of thymocytes to the single CD8+ mature phenotype, indicating that thymocytes are positively selected

• however, double transgenic mice are able to respond by proliferation to LCMV antigens at the same magnitude as single Tg(TcrLCMV)327Sdz mice and cytotoxic T lymphocytes are able to respond to LCMV infection as in controls

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:209714 )

N • mice do not develop diabetes and exhibit normal islet architecture with no evidence of insulitis

hyperglycemia ( J:81285 )

• mice immunized with lymphocytic choriomeningitis virus (LCMV) develop hyperglycemia 3 to 4 days after infection

• a variant LCM virus, 8.7, does not induce diabetes in mice

increased urine glucose level ( J:81285 )

• mice immunized with LCMV develop glucosuria 3 to 4 days after infection

immune system

abnormal B cell selection ( J:81285 )

• skewing of thymocytes to the single CD8+ mature phenotype, indicating that thymocytes are positively selected

• however, double transgenic mice are able to respond by proliferation to LCMV antigens at the same magnitude as single Tg(TcrLCMV)327Sdz mice and cytotoxic T lymphocytes are able to respond to LCMV infection as in controls

renal/urinary system

increased urine glucose level ( J:81285 )

• mice immunized with LCMV develop glucosuria 3 to 4 days after infection

Genotype
MGI:5569019

cx2

• Allelic Composition: Tg(Ins1-Cd80)378Psoh/0; Tg(Ins2-GP)#Psoh/0; Tg(TcrLCMV)327Sdz/0
• Genetic Background: involves: C57BL/6 * DBA/2 * FVB/N

mortality/aging

premature death ( J:209714 )

• hyperglycemia is followed by ketosis, wasting, and death

endocrine/exocrine glands

absent pancreatic beta cells ( J:209714 )

• complete beta-cell destruction

insulitis ( J:209714 )

• ongoing insulitis is seen at 10 weeks of age, with a diminished, but still present, mass of beta cells

• 4 week old mice show a mixture of a few cD8+ and CD4+ T lymphocytes and an increase in the number of macrophages surrounding pancreatic islets, while 9 week old mice show severe insulitis and periinsulitis with CD8+ and CD4+ T lymphocytes, macrophages and B lymphocytes infiltrating the islets

periinsulitis ( J:209714 )

• 9 week old mice show severe periinsulitis

growth/size/body

cachexia ( J:209714 )

• hyperglycemia is wasting

homeostasis/metabolism

hyperglycemia ( J:209714 )

• mice spontaneously become diabetic with a median age of hyperglycemia onset of 9-10 weeks of age

ketosis ( J:209714 )

• hyperglycemia is followed by ketosis

immune system

insulitis ( J:209714 )

• ongoing insulitis is seen at 10 weeks of age, with a diminished, but still present, mass of beta cells

• 4 week old mice show a mixture of a few cD8+ and CD4+ T lymphocytes and an increase in the number of macrophages surrounding pancreatic islets, while 9 week old mice show severe insulitis and periinsulitis with CD8+ and CD4+ T lymphocytes, macrophages and B lymphocytes infiltrating the islets

periinsulitis ( J:209714 )

• 9 week old mice show severe periinsulitis

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
type 1 diabetes mellitus		DOID:9744	OMIM:222100	J:209714

Genotype
MGI:5569021

cx3

• Allelic Composition: Tg(Ins1-Cd80)378Psoh/0; Tg(Ins2-GP)#Psoh/0
• Genetic Background: involves: C57BL/6 * FVB/N

homeostasis/metabolism

hyperglycemia ( J:209714 )

• mice infected with a recombinant vaccinia virus expressing GP develop diabetes 6-10 days after infection

• however, mice do not develop spontaneous diabetes and exhibit normal islet architecture with no evidence of isulitis

Genotype
MGI:5568710

tg4

• Allelic Composition: Tg(Ins2-GP)#Psoh/0
• Genetic Background: involves: C57BL/6

mortality/aging

premature death ( J:81285 )

• mice immunized with LCMV die 15-20 days after infection

homeostasis/metabolism

hyperglycemia ( J:81285 )

• mice immunized with lymphocytic choriomeningitis virus (LCMV) develop hyperglycemia 9-11 days after infection

• treatment with either CD4 or CD8 antibodies one day before or after LCMV infection results in no signs of increased glucose levels

• treatment with poly-IC to induce IFN-alpha and IFN-beta or with IFN-alpha to enhance expression of class I and class II MHC does not induce diabetes in mutants

increased urine glucose level ( J:81285 )

• mice immunized with LCMV develop glucosuria 9-11 days after infection

immune system

insulitis ( J:81285 )

• mice immunized with LCMV show lymphocytic infiltration of the islets of Langerhans; CD4+ T cells are seen around the islets of Langerhans while CD8+ T cells infiltrate the entire islets of the pancreas

endocrine/exocrine glands

insulitis ( J:81285 )

• mice immunized with LCMV show lymphocytic infiltration of the islets of Langerhans; CD4+ T cells are seen around the islets of Langerhans while CD8+ T cells infiltrate the entire islets of the pancreas

renal/urinary system

increased urine glucose level ( J:81285 )

• mice immunized with LCMV develop glucosuria 9-11 days after infection