Analysis Tools
mortality/aging
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• homozygous embryos are recovered in expected Mendelian ratios at E7.5 and E8.5; however, no embryos are obtained at E9.5, E12.5 or E18.5
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• homozygous embryos are recovered in expected ratios at E7.5 but arrest prior to gastrulation; no homozygous embryos are recovered at E9.5
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embryo
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• by E8.5, all structures typical of wild-type embryos, including headfolds, heart, somites and allantois, are missing
• however, most embryos display a clear extra-embryonic/embryonic boundary indicating that pre-gastrulation events leading to egg cylinder formation are normal
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• embryos fail to initiate gastrulation; even at E8.5, features typical of gastrulating embryos (primitive streak, node, and early headfolds) are not observed
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• all embryos show morphological defects resulting in developmental failure
(J:277394)
• embryos arrest prior to gastrulation
(J:279207)
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• at E7.5, embryos resemble E6.5 wild-type controls in overall size and shape
• by E8.5, embryos have increased in size (relative to E7.5) but remain smaller than wild-type controls
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• at E7.5, embryos are smaller than wild-type controls
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• at E7.5, many embryos show an abnormal buildup of cells in the embryonic portion of the embryo, while others show a severe reduction of the proximal-distal length of the embryo proper
• the embryonic compartments are filled with small and abnormally rounded cells
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• epiblast cells appear to adopt a uniform atypical shape
• at E7.5, no obvious Oct4+ cells are observed in the epiblast except for a subset of distally located rounded cells
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• at E7.5, significantly fewer proliferation marker phospho-histoneH3-positive (PH3+) cells are observed; only mutant cells that are PH3+ are those that are also Oct4+
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• no head folds are observed even at E8.5
(J:277394)
• no head folds are present at E7.5
(J:279207)
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• no embryonic node is observed at E8.5
(J:277394)
• no embryonic node is present at E7.5
(J:279207)
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• at E7.5, Brachyury (T) expression is absent indicating failure of primitive streak initiation
(J:277394)
• at E7.5, no molecular or morphological evidence of primitive streak formation is observed
(J:279207)
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• at E8.5
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• the extra-embryonic compartments are filled with small and abnormally rounded cells
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• at E8.5
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growth/size/body
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• at E7.5, embryos resemble E6.5 wild-type controls in overall size and shape
• by E8.5, embryos have increased in size (relative to E7.5) but remain smaller than wild-type controls
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• at E7.5, embryos are smaller than wild-type controls
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cellular
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• at E7.5, significantly fewer proliferation marker phospho-histoneH3-positive (PH3+) cells are observed; only mutant cells that are PH3+ are those that are also Oct4+
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cardiovascular system
absent heart
(
J:277394
)
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• at E8.5
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