Phenotypes associated with this allele

• Allele Symbol: Tg(Cck-cre,-lacZ)1Mini
• Allele Name: transgene insertion 1, Liliana Minichiello
• Allele ID: MGI:5574585
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Ntrk2^tm2Kln/Ntrk2^tm2Kln Tg(Cck-cre,-lacZ)1Mini/0	involves: 129S2/SvPas * C57BL/6 * CBA	MGI:5575864

Genotype
MGI:5575864

cn1

• Allelic Composition: Ntrk2^tm2Kln/Ntrk2^tm2Kln; Tg(Cck-cre,-lacZ)1Mini/0
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

increased body weight ( J:210229 )

• mutants show greater weight gain in the first week on a high fat diet compared to controls on the same diet

• treatment with mifepristone stabilizes mutant body weight

increased susceptibility to weight gain ( J:210229 )

• mutants gain more weight than controls on the same diet, despite no increase in food intake

increased susceptibility to age related obesity ( J:210229 )

• males develop obesity around 4 months of age, while females exhibit this around 5 months

increased liver weight ( J:210229 )

• gonadal white adipose tissue fat pads are significantly increased in males and females

endocrine/exocrine glands

increased adrenal gland zona fasciculata size ( J:210229 )

• from 3 months of age, thickness is increased becoming highly significant at 5 months, compared to controls

adrenal cortical hyperplasia ( J:210229 )

• beginning at 3 months, hypertrophy is observed, becoming significant by 5 months

adipose tissue

adipose tissue phenotype ( J:210229 )

N • interscapular brown adipose tissue are found in mutants and controls

increased total body fat amount ( J:210229 )

• fat mass is significantly increased in 3 month-old and 9 month-old male mutants; however, fat-free mass is unaltered

abnormal adipose tissue distribution ( J:210229 )

• mutants have increased fat accumulation in the head/neck region

increased gonadal fat pad weight ( J:210229 )

• gonadal white adipose tissue fat pads are significantly increased in males and females

increased abdominal fat pad weight ( J:210229 )

• mesenteric adipose tissue fat pads are significantly increased in males and females

behavior/neurological

behavior/neurological phenotype ( J:210229 )

N • no alterations in circadian rhythm or cumulative food intake at 2 or 8 months of age

N • on a high fat diet, mutants exhibit decreased food intake similarly to controls

N • open field activity and elevated plus maze behavior (indicator of anxiety-like behavior) does not differ between mutants and controls at any age

abnormal circadian behavior ( J:210229 )

• home cage activity is significantly increased during the dark phase in 8 month-old obese mutants

nervous system

abnormal GABAergic neuron physiology ( J:210229 )

• GABAergic output of Cck interneurons onto Crh neurons is reduced; increased neuronal activity results

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:210229 )

N • oxygen consumption and carbon dioxide production rates, as well as respiratory exchange ratio and energy expenditure are similar

increased circulating insulin level ( J:210229 )

• signicantly elevated in older obese mice

increased circulating leptin level ( J:210229 )

• signicantly elevated in older obese mice

impaired glucose tolerance ( J:210229 )

• observed in older obese mice

abnormal lipid level ( J:210229 )

• mutants show increased de novo lipogenesis in the liver

abnormal circulating corticosterone level ( J:210229 )

• afternoon levels are increased compared to controls

liver/biliary system

increased liver weight ( J:210229 )

• gonadal white adipose tissue fat pads are significantly increased in males and females

hepatic steatosis ( J:210229 )

• aged mice (5 months) show increased hepatic lipid stores compared to controls