Phenotypes associated with this allele

• Allele Symbol: Tg(LHX3-cre)#Sjr
• Allele Name: transgene insertion, Simon J Rhodes
• Allele ID: MGI:5576209
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gt(ROSA)26Sor^tm1(DTA)Lky/Gt(ROSA)26Sor^+ Tg(LHX3-cre)#Sjr/0	involves: 129P2/OlaHsd * BALB/c * C3H * C57BL/6	MGI:5576239

Genotype
MGI:5576239

cn1

• Allelic Composition: Gt(ROSA)26Sor^tm1(DTA)Lky/Gt(ROSA)26Sor⁺; Tg(LHX3-cre)#Sjr/0
• Genetic Background: involves: 129P2/OlaHsd * BALB/c * C3H * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

lethality throughout fetal growth and development, incomplete penetrance ( J:211401 )

• viable double mutant embryos are recovered in significantly reduced numbers at E14.5 and E17.5 compared to cre-negative/R26^DTA embryos

growth/size/body

decreased body size ( J:211401 )

• the few animals surviving past birth are smaller than littermates

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:211401 )

N • ACTH hormone levels are not significantly altered in mutants at E14.5

N • at E17.5 and P0, numbers of ACTH, TPIT, PIT-1 (ie. thyrotropes, somatotropes, and lactotropes), PRL, and GH-positive cells in the anterior pituitary are not significantly reduced compared to controls

abnormal adenohypophysis morphology ( J:211401 )

• at E14.5, cells positive for alphaGSU expression (ie. gonadotrophs) are reduced in the caudomedial portion of the anterior pituitary

• GATA2-positive cells are absent from the caudomedial region of the pituitary but are still present in the rostral tip

decreased gonadotroph cell number ( J:211401 )

• at E14.5, cells positive for alphaGSU expression (ie. gonadotropes) are reduced in the caudomedial portion of the anterior pituitary

• in animals at P0, LH beta-positive gonadotrope cells are decreased by about 90% compared to controls; FSH beta-positive gonadotrope cells are decreased slightly also

• at P0, numbers of LH/SF1 or FSH/SF1 double positive cells are significantly decreased

• at P0, about 90% of bihormonal (LH beta/FSH beta positive) cells are missing compared to controls

nervous system

abnormal adenohypophysis morphology ( J:211401 )

• at E14.5, cells positive for alphaGSU expression (ie. gonadotrophs) are reduced in the caudomedial portion of the anterior pituitary

• GATA2-positive cells are absent from the caudomedial region of the pituitary but are still present in the rostral tip

decreased gonadotroph cell number ( J:211401 )

• at E14.5, cells positive for alphaGSU expression (ie. gonadotropes) are reduced in the caudomedial portion of the anterior pituitary

• in animals at P0, LH beta-positive gonadotrope cells are decreased by about 90% compared to controls; FSH beta-positive gonadotrope cells are decreased slightly also

• at P0, numbers of LH/SF1 or FSH/SF1 double positive cells are significantly decreased

• at P0, about 90% of bihormonal (LH beta/FSH beta positive) cells are missing compared to controls

abnormal spinal cord morphology ( J:211401 )

• numbers of Lhx-positive interneurons are decreased in the V2 interneuron region of the spinal cord at E14.5, but populations of motorneurons (ventral) are not affected

• at E14.5, V2a interneurons expressing LHX3 and CHX10 are reduced in number

reproductive system

male infertility ( J:211401 )

♂ • of 2 surviving male mutants, one was infertile