Analysis Tools
cellular
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• 6 hours after infection with Legionella pneumophila lacking the flagellin A gene, dendritic cells (DCs) from transgenic mice did not exhibit the condensed and fragmented nuclei observed in wild-type C57BL/6 control DCs; failure of infected trangenic DCs to undergo apoptosis was confirmed by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), whereby >30% of control but <5% of transgenic DCs stained positive for nuclear DNA fragmentation.
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hematopoietic system
immune system
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• Legionella pneumophila lacking the flagellin A gene (DflaA) are able to replicate in dendritic cells of transgenic but not of wild-type C57BL/6 mice, as assayed by the ratio of the number of colony forming units (CFU) of bacteria recovered at 36 hr/1 hr after infection and by the proportion of infected dendritic cells with vacuoles containing replicating bacteria, visualized by fluorescent labeling, at 10 hr vs. 1 hr incubation.
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• 6 hours after infection with Legionella pneumophila lacking the flagellin A gene, dendritic cells (DCs) from transgenic mice did not exhibit the condensed and fragmented nuclei observed in wild-type C57BL/6 control DCs; failure of infected trangenic DCs to undergo apoptosis was confirmed by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), whereby >30% of control but <5% of transgenic DCs stained positive for nuclear DNA fragmentation.
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reproductive system
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• female mice hemizygous for this transgene exhibit very low fertility, so that all breeding of the line has employed wild-type females and hemizygous transgenic males.
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growth/size/body
