All Phenotypes Mcrs1<tm1.1(KOMP)Vlcg> MGI Mouse

embryonic lethality before implantation, complete penetrance ( J:279207 , J:285737 )

• although normal looking blastocysts are present in expected Mendelian ratios at E3.5, no homozygous embryos or excess resorption sites are observed at E7.5 (J:279207)

• homozygous embryos cannot be recovered in vivo after E3.5 (J:285737)

decreased inner cell mass proliferation ( J:285737 )

• although blastocysts hatch from the zona pellucida with apparent trophectoderm (TE) and primitive endoderm (PrE) cells, inner cell mass (ICM) cells fail to form a typical outgrowth colony after 3 days in culture, suggesting impaired signaling from or proliferation of the ICM

abnormal embryonic epiblast morphology ( J:285737 )

• blastocysts flushed at E3.5 and cultured overnight contain a significantly decreased total number and percentage of NANOG+ epiblast cells

• 4 of 7 blastocysts exhibit very few epiblast cells while the remaining 3 contain no NANOG+ cells within the ICM, indicating defective epiblast allocation

• however, the number and percentage of SOX17+ primitive endoderm (PE) cells is normal

abnormal inner cell mass morphology ( J:285737 )

• immunofluorescence analysis of POU5F1 (aka OCT4) shows that the percentage of POU5F1+ ICM cells is severely decreased, with only ~45% of the appropriate number of ICM cells present

• however, the total cell number per blastocyst is similar to that in controls, and no increase in Trp53-dependent apoptosis or changes in histone H4 acetylation (H4K5 + K8 + K12 + K16) are noted in the ICM

absent inner cell mass ( J:285737 )

• in vitro outgrowth assays show absence of ICM after 3 days in culture

abnormal trophectoderm morphology ( J:285737 )

• immunofluorescence analysis of the trophectoderm (TE) marker CDX2 shows that the percentage of CDX2+ TE cells is significantly increased

decreased inner cell mass proliferation ( J:285737 )

• although blastocysts hatch from the zona pellucida with apparent trophectoderm (TE) and primitive endoderm (PrE) cells, inner cell mass (ICM) cells fail to form a typical outgrowth colony after 3 days in culture, suggesting impaired signaling from or proliferation of the ICM

failure of embryo implantation ( J:279207 , J:285737 )

• in vitro outgrowth assays show that ICM/epiblast cells fail to form a typical outgrowth colony after 3 days in culture (J:279207)

• homozygous embryos show normal morphology at the blastocyst stage but cannot be recovered at gastrulation, suggesting implantation failure (J:285737)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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