Phenotypes associated with this allele

• Allele Symbol: Cst3^{tm1(CST3*L68Q)Cnw}
• Allele Name: targeted mutation 1, Gail A Cornwall
• Allele ID: MGI:5586715
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Cst3^tm1(CST3*L68Q)Cnw/Cst3^+	involves: 129P2/OlaHsd * C57BL/6	MGI:5586716

Genotype
MGI:5586716

ht1

• Allelic Composition: Cst3^{tm1(CST3*L68Q)Cnw}/Cst3⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal male germ cell morphology ( J:210672 )

♂ • cauda epididymal spermatozoa exhibit reduced cell viability compared to wild-type spermatozoa and are often present in large agglutinated clumps

asthenozoospermia ( J:210672 )

♂ • spermatozoa show poor motility with little to no forward progression

homeostasis/metabolism

amyloidosis ( J:210672 )

♂ • increase in fibrillar structures characteristic of cystatin C amyloid are present in epididymal fluid and distinct forms of cystatin C amyloid are found that are not seen in wild-type mice

reproductive system

abnormal male germ cell morphology ( J:210672 )

♂ • cauda epididymal spermatozoa exhibit reduced cell viability compared to wild-type spermatozoa and are often present in large agglutinated clumps

asthenozoospermia ( J:210672 )

♂ • spermatozoa show poor motility with little to no forward progression

reduced male fertility ( J:210672 )

♂ • mutant males mated with wild-type females generate only 0.2 pups/litter compared to 4.6 pups/litter for controls indicating reduced male fertility

♂ • spermatozoa from mutant males are unable to fertilize cumulus oocyte complexes or oocytes after cumulus cell removal

♂ • spermatozoa, however, are able to fertilize oocytes when the zona pellucida is removed

impaired binding of sperm to zona pellucida ( J:210672 )

♂ • spermatozoa do not bind as efficiently to the zona pellucida as wild-type spermatozoa