All Phenotypes Ift27<tm1c(EUCOMM)Hmgu> MGI Mouse

reproductive system phenotype ( J:251964 )

N	• males exhibit no significant differences in testis weight or testis/body weight ratio relative to control males

decreased sperm progressive motility ( J:251964 )

• only ~2% of epididymal sperm are motile but show a dramatic loss of progressive motility

asthenozoospermia ( J:251964 )

• only ~2% of epididymal sperm are motile but their motility, calculated as curvilinear velocity (VCL), is severely reduced

immotile sperm ( J:251964 )

• vast majority of epididymal sperm are immotile

abnormal testis physiology ( J:251964 )

• testicular expression levels of IFT25 (the IFT27 binding partner) and IFT81 (a component of IFT-B complex) are significantly reduced

abnormal spermatogenesis ( J:251964 )

• adult males show multiple signs of spermatogenesis failure during spermiogenesis

oligozoospermia ( J:251964 )

• epididymal sperm count is severely reduced

teratozoospermia ( J:251964 )

• sperm collected from the cauda epididymides show multiple abnormalities, including short, bent tails and round heads

• however, sperm lipid raft distribution appears to be normal

abnormal sperm flagellum morphology ( J:251964 )

• very few sperm appear to have a smooth tail; different light densities are observed along the whole tail

• most sperm have vesicles/vacuoles in some area along the flagella

• some epididymal sperm flagella have a distorted/damaged cell membrane

• however, branched sperm flagella are never observed

abnormal outer dense fiber morphology ( J:251964 )

• almost all epididymal sperm have misplaced or missing outer dense fibers (ODFs)

abnormal sperm axoneme morphology ( J:251964 )

• TEM analysis showed that almost all epididymal sperm show a disrupted 9 + 2 core axoneme structure and a disorganized axoneme microtubule array

• very few axoneme structures are detected in the seminiferous tubules

abnormal sperm end piece morphology ( J:251964 )

• some sperm exhibit swollen tail tips

abnormal sperm flagellum thickness ( J:251964 )

• uneven thickness of sperm tails in some areas

disorganized sperm mitochondrial sheath ( J:251964 )

• TEM analysis of epididymal sperm showed that the mitochondria sheath is disorganized

abnormal sperm fibrous sheath morphology ( J:251964 )

• TEM analysis of epididymal sperm showed that the fibrous sheath is disorganized

kinked sperm flagellum ( J:251964 )

• bent sperm tails are observed

short sperm flagellum ( J:251964 )

abnormal sperm head morphology ( J:251964 )

• round and other misshapen sperm heads are observed

globozoospermia ( J:251964 )

• round sperm heads are observed

abnormal spermiation ( J:251964 )

• very few developed sperm are found in the lumen of seminiferous tubules

arrest of spermiogenesis ( J:251964 )

• cauda epididymis lumen is filled with abnormal sperm heads, cytoplasm bodies, sloughed round spermatids, sloughed aggregates of elongating spermatids, numerous detached sperm heads and abnormal short sperm tails that appear disorganized

male infertility ( J:251964 )

• when bred with adult wild-type females of known fertility, none of the 6-wk-old or adult males tested sired any pups/litters during a 2-month breeding period, indicating complete male sterility