Allele Symbol Allele Name Allele ID |
Gpr139tm1.1(KOMP)Vlcg targeted mutation 1.1, Velocigene MGI:5605824 |
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Summary |
2 genotypes
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Data Sources
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♀ | phenotype observed in females |
♂ | phenotype observed in males |
N | normal phenotype |
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♀ | phenotype observed in females |
♂ | phenotype observed in males |
N | normal phenotype |
• basal firing rates are selectively increased in locus coeruleus (LC) neurons but not in medial habenula (MHb) neurons
• firing of MHb neurons is significantly reduced by DAMGO (a synthetic enkephalin-mimetic peptide) at a low concentration (0.3 um), which has no significant effect in wild-type MHb neurons
• MHb neurons show a significantly greater net inhibition of neuronal firing by DAMGO both at a low (0.3 um) and high (1 uM) concentration
• following washout of DAMGO (1 uM), recovery kinetics of MHb neurons is significantly slower than that in wild-type MHb neurons, indicating greater susceptibility to opioid inhibition
• firing of LC neurons is significantly reduced by morphine at 0.1 um, which has no significant effect in wild-type LC neurons
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N |
• at ~4 months of age, mice exhibit normal baseline learning, nociception, locomotor activity, habituation to an unfamiliar environment and motor coordination relative to wild-type controls
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• mice exhibit significantly reduced withdrawal signs (diarrhea, jumps, dog shakes, paw tremor, back walking, tremor and ptosis) and weight loss due to naloxone-precipitated somatic withdrawal after chronic morphine exposure
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• in a conditioned place preference paradigm, mice exhibit augmented responses to the rewarding effects of morphine, in agreement with increased opioid sensitivity of MHb neurons
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• mice exhibit enhanced morphine-induced analgesia in thermal (hot plate and tail immersion) and mechanical (Von Frey) pain paradigms, as shown by increases in both maximal response and effect duration across multiple morphine doses
• administration of JNJ-63533054 (a GPR139 agonist) fails to diminish morphine-induced (10mg/kg) analgesia in a dose-dependent manner in the thermal and mechanical pain paradigms, unlike in wild-type control mice
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N |
• at ~4 months of age, mice exhibit normal health, body weight and body composition relative to wild-type controls
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/19/2024 MGI 6.24 |
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