Phenotypes associated with this allele

• Allele Symbol: Afdn^{tm1c(EUCOMM)Hmgu}
• Allele Name: targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• Allele ID: MGI:5607284
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Afdn^tm1c(EUCOMM)Hmgu/Afdn^tm1c(EUCOMM)Hmgu Emx1^tm1(cre)Krj/Emx1^+	involves: 129S2/SvPas * C57BL/6J * C57BL/6N	MGI:5607285

Genotype
MGI:5607285

cn1

• Allelic Composition: Afdn^{tm1c(EUCOMM)Hmgu}/Afdn^{tm1c(EUCOMM)Hmgu}; Emx1^tm1(cre)Krj/Emx1⁺
• Genetic Background: involves: 129S2/SvPas * C57BL/6J * C57BL/6N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

embryo

abnormal embryonic neuroepithelium morphology ( J:215584 )

• marker analysis indicates disruption of adherens junctions in neuroepithelial cells at E13.5

nervous system

abnormal radial glial cell morphology ( J:215584 )

• adherens junctions between radial glial cells are disrupted

increased radial glial cell number ( J:215584 )

• increase in the proliferation of and in the number of Pax6+ radial glial cells at E13.5

abnormal embryonic neuroepithelium morphology ( J:215584 )

• marker analysis indicates disruption of adherens junctions in neuroepithelial cells at E13.5

abnormal telencephalon development ( J:215584 )

• accumulations of neurons at the surface of the ventricle and below the intermediate zone in which they form rosette-like structures at E16.5

thin cortical plate ( J:215584 )

• at E16.5

abnormal cortical ventricular zone morphology ( J:215584 )

• structure of the ventricular zone is disorganized

increased brain size ( J:215584 )

• brain is larger at P25

abnormal hippocampus morphology ( J:215584 )

• hippocampal structure is less well organized

abnormal cerebral cortex morphology ( J:215584 )

• mice exhibit subcortical band heterotopia, also known as double cortex, a malformation in which heterotopic gray matter is interposed between zones of white matter

• the cortex contains a thin layer of cells near the meninges and a disorganized mass of cells heterotopically localized deeper within the cortex

abnormal neocortex morphology ( J:215584 )

• severe disorganization of the neocortex at P15

• the thickness of the developing neocortex is increased at E13.5 and the ventricular surface is disrupted and irregular

• the neocortex is severely disorganized and hyperplastic at E16.5

• proliferating cells (Pax6+ RGCs and Tbr2+ intermediate progenitors) are broadly distributed throughout the entire thickness of the nascent neocortex at E13.5 and E16.5 compared to being confined to ventricular zone (VZ) and subventricular zone (SVZ) of controls

• at E13.5, Tuj1+ neurons are distributed irregularly with some of them occupying positions within the VZ and SVZ

increased neocortex size ( J:215584 )

• at P15, the neocortex is increased in size

• the thickness of the developing neocortex is increased at E13.5

abnormal stratification in cerebral cortex ( J:215584 )

• some subtypes of cortical projection neurons are generated but their assembly into neocortical cell layers is perturbed

abnormal nervous system tract morphology ( J:215584 )

• massive perturbations in axonal tracts

increased neuronal precursor cell number ( J:215584 )

• proliferation of cortical progenitor cells is increased, with the number of Ki67+ cells increased at E13.5 by about 50% in the neocortex, with an approximately equal increase in the number of Pax6+ radial glial cells and Tbr2+ intermediate progenitors

ectopic neuron ( J:215584 )

• the cortex contains a disorganized mass of cells heterotopically localized deeper within the cortex

cellular

abnormal cell cycle ( J:215584 )

• cell cycle regulation of cortical progenitor cells is altered in embryos

abnormal radial glial cell morphology ( J:215584 )

• adherens junctions between radial glial cells are disrupted

increased radial glial cell number ( J:215584 )

• increase in the proliferation of and in the number of Pax6+ radial glial cells at E13.5