Phenotypes associated with this allele

• Allele Symbol: Mpl^tm2.1Wsa
• Allele Name: targeted mutation 2.1, Warren S Alexander
• Allele ID: MGI:5607579
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Mpl^tm2.1Wsa/Mpl^tm2.1Wsa Tg(Pf4-icre)Q3Rsko/0	involves: C57BL/6	MGI:5607586

Genotype
MGI:5607586

cn1

• Allelic Composition: Mpl^tm2.1Wsa/Mpl^tm2.1Wsa; Tg(Pf4-icre)Q3Rsko/0
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

increased hematopoietic stem cell proliferation ( J:208120 )

• total numbers of clonogenic hemopoietic progenitor cells in the bone marrow and spleen are increased indicating that mice develop features of myeloproliferation

enlarged spleen ( J:208120 )

abnormal common myeloid progenitor cell morphology ( J:208120 )

• total number of myeloid progenitor cells in the bone marrow and spleen is increased, with a particular increase in the number of blast colony-forming cells

• blast cell colonies show increased propensity to formation of secondary colonies, particularly secondary blast cell and megakaryocyte colonies

• 2- to 4-fold increase in bone marrow LSK cells, Lin-cKit+Sca1- myeloid progenitors, pregranulocyte-macrophage progenitors, granulocyte-macrophage (GM) progenitors, and CD150+CD9(hi) and CD150+FcgammaR+ bipotential erythroid-megakaryocyte progenitor populations

abnormal megakaryocyte morphology ( J:208120 )

• increase in the proportion of megakaryocytes with ploidy of 16N or greater

increased megakaryocyte cell number ( J:208120 )

• mice develop megakaryocytosis in the bone marrow and spleen

• clonogenic assays of bone marrow and spleen cells show an increase in the numbers of megakaryocyte colony-forming cells

decreased erythroid progenitor cell number ( J:208120 )

• reduction in erythroid progenitors (preCFU-E and CFU-E)

thrombocytosis ( J:208120 )

• mice develop thrombocytosis, with a 10-fold increase in the number of circulating platelets

cellular

increased hematopoietic stem cell proliferation ( J:208120 )

• total numbers of clonogenic hemopoietic progenitor cells in the bone marrow and spleen are increased indicating that mice develop features of myeloproliferation

immune system

enlarged spleen ( J:208120 )

growth/size/body

enlarged spleen ( J:208120 )