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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Vps35tm1.1Mjff
targeted mutation 1.1,The Michael J Fox Foundation
MGI:5607779
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Vps35tm1.1Mjff/Vps35tm1.1Mjff B6.Cg-Vps35tm1.1Mjff MGI:7378801
ht2
Vps35tm1.1Mjff/Vps35+ B6.Cg-Vps35tm1.1Mjff MGI:7378804


Genotype
MGI:7378801
hm1
Allelic
Composition
Vps35tm1.1Mjff/Vps35tm1.1Mjff
Genetic
Background
B6.Cg-Vps35tm1.1Mjff
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Vps35tm1.1Mjff mutation (1 available); any Vps35 mutation (45 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• mitochondria from neurons from the substanta nigra pars compacta of 14-month-old mice are shorter, rounder, smaller and less dense than controls
• basal OCR (oxygen consumption rate), maximal OCR and spare OCR are reduced in 15-month-old mice

behavior/neurological
N
• no differences in rotarod test or grip strength
• decrease in latency to fall in rotarod test following MTPT (neurotoxin) treatment in 3-month-old mice
• increased time to cross 9 mm beam following MTPT treatment in 3-month-old mice
• reduction in mean speed and travel distance in open field test is observed in 14-month-old mice as compared to wild-type
• increase in time to cross beam in 14-month-old mice as compared to controls

hematopoietic system
• increased microgliosis in substanta nigra pars compacta (but not striatum) of MPTP-treated 3-month-old mice as compared to MPTP-treated controls

homeostasis/metabolism
• Increased dopamine release in dorsolateral striatal slices as measured by fast scan cyclic voltammetry
• Decay time of dopamine transient is slower in dorsolateral striatal slices
• Increased dopamine turnover as measured by ration of dopamine metabolites to dopamine
• In response to a D2 agonist, quinpirole, dorsolateral striatal slices exhibit a more rapid inhibition of dopamine release

nervous system
• increased microgliosis in substanta nigra pars compacta (but not striatum) of MPTP-treated 3-month-old mice as compared to MPTP-treated controls
• astrogliosis observed in 15-16-month-old mice in the substanta nigra pars compacta, but not in the striatum
• neurons contain increased numbers of lysosomes with dense bodies or other inclusions in substanta nigra pars compacta of 14-month-old mice
• decreased levels of TH-positive dopaminergic neurons (12%) in substanta nigra pars compacta of 15-16-month-old mice
• decreased levels of TH-positive dopaminergic nerve terminals (25%) in striatum of 15-16-month-old mice
• decreased TH-positive neurons and TH-positive striatal fibers in MPTP-treated 3-month-old mice as compared to MPTP-treated controls (36% vs. 51%)
• dystrophic myelinated axons contain autophagic vacuoles in substanta nigra pars compacta of 14-month-old mice
• accumulation and aggregation of alpha-synuclein is observed in substanta nigra pars compacta of 15-16-month-old mice, no tau pathology is observed

immune system
• increased microgliosis in substanta nigra pars compacta (but not striatum) of MPTP-treated 3-month-old mice as compared to MPTP-treated controls

pigmentation
• increased numbers of lipofuscins in substanta nigra pars compacta of 14-month-old mice

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
Parkinson's disease DOID:14330 OMIM:PS168600
J:303283




Genotype
MGI:7378804
ht2
Allelic
Composition
Vps35tm1.1Mjff/Vps35+
Genetic
Background
B6.Cg-Vps35tm1.1Mjff
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Vps35tm1.1Mjff mutation (1 available); any Vps35 mutation (45 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism
• Increased dopamine release in dorsolateral striatal slices as measured by fast scan cyclic voltammetry; in heterozygote increase is less than in homozygote, but higher than in wild-type
• Decay time of dopamine transient is slower in dorsolateral striatal slices
• Increased dopamine turnover as measured by ration of dopamine metabolites to dopamine
• In response to a D2 agonist, quinpirole, dorsolateral striatal slices exhibit a more rapid inhibition of dopamine release





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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory