Phenotypes associated with this allele

• Allele Symbol: Rhoj^tm1.2Auem
• Allele Name: targeted mutation 1.2, Akiyoshi Uemura
• Allele ID: MGI:5608669
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rhoj^tm1.2Auem/Rhoj^tm1.2Auem	B6.Cg-Rhoj^tm1.2Auem	MGI:5608672
cx2	Rhoj^tm1.2Auem/Rhoj^tm1.2Auem Tg(MMTV-PyVT)634Mul/0	involves: C57BL/6 * CBA/JNCrlj * FVB/N	MGI:5608671

Genotype
MGI:5608672

hm1

• Allelic Composition: Rhoj^tm1.2Auem/Rhoj^tm1.2Auem
• Genetic Background: B6.Cg-Rhoj^tm1.2Auem

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

neoplasm

abnormal tumor vascularization ( J:208154 )

• decreased vascular densities, increase in vessel disruptions, and reduced vascular spouting in tumors formed by injection of Lewis lung carcinoma cells

• coverage of tumor vessels with alpha-SMA+ mural cells and basement membrane is decreased and vascular permeability is increased

• vascular densities are also reduced in tumors formed by injected B16F10 melanoma cells

decreased metastatic potential ( J:208154 )

• in tumors formed by injection of Lewis lung carcinoma cells

abnormal tumor morphology ( J:208154 )

• tumors formed by injection of Lewis lung carcinoma cells show an increase in hemorrhagic foci and increased hypoxia with extensive apoptosis in the center of the tumor

decreased tumor growth/size ( J:208154 )

• decreased growth of injected Lewis lung carcinoma cells

• tumor growth is also delayed for injected B16F10 melanoma cells

• impact of cisplatin, VEGF-A blockade, or combretastatin-A4-phosphate on Lewis lung carcinoma cell tumor growth/necrosis is enhanced compared to wild-type controls

homeostasis/metabolism

increased physiological sensitivity to xenobiotic ( J:208154 )

• impact of cisplatin on Lewis lung carcinoma cell tumor growth/necrosis is enhanced compared to wild-type controls

• impact of combretastatin-A4-phosphate treatment on inhibition of tumor growth and inhibition of tumor vascularization is enhanced

cardiovascular system

cardiovascular system phenotype ( J:208154 )

N • no differences from wild-type controls in vascular morphology or integrity are found in untreated mice

abnormal tumor vascularization ( J:208154 )

• decreased vascular densities, increase in vessel disruptions, and reduced vascular spouting in tumors formed by injection of Lewis lung carcinoma cells

• coverage of tumor vessels with alpha-SMA+ mural cells and basement membrane is decreased and vascular permeability is increased

• vascular densities are also reduced in tumors formed by injected B16F10 melanoma cells

internal hemorrhage ( J:208154 )

• tumors formed by injection of Lewis lung carcinoma cells show an increase in hemorrhagic foci

Genotype
MGI:5608671

cx2

• Allelic Composition: Rhoj^tm1.2Auem/Rhoj^tm1.2Auem; Tg(MMTV-PyVT)634Mul/0
• Genetic Background: involves: C57BL/6 * CBA/JNCrlj * FVB/N

neoplasm

abnormal tumor vascularization ( J:208154 )

♀ • decreased tumor vascular densities and vascular sprouting in peri- and intratumoral regions

♀ • vascular morphology in intratumoral regions appear more disrupted, vascular permeability is increased and pericyte support is reduced

decreased mammary gland tumor incidence ( J:208154 )

♀ • decreased development of spontaneous mammary tumor nodules at 14 weeks of age and median time to palpable tumor development is delayed by approximately 2 weeks

decreased metastatic potential ( J:208154 )

♀ • average number of metastatic tumor colonies in the lung is reduced

decreased tumor growth/size ( J:208154 )

♀ • tumor progression and invasion is delayed

♀ • average tumor size is reduced

homeostasis/metabolism

delayed wound healing ( J:208154 )

• 23% delayed wound closure, 48% reduced vascular density and 39% decreased granulation area in wound regions in a punch wound healing assay

cardiovascular system

abnormal tumor vascularization ( J:208154 )

♀ • decreased tumor vascular densities and vascular sprouting in peri- and intratumoral regions

♀ • vascular morphology in intratumoral regions appear more disrupted, vascular permeability is increased and pericyte support is reduced

endocrine/exocrine glands

decreased mammary gland tumor incidence ( J:208154 )

♀ • decreased development of spontaneous mammary tumor nodules at 14 weeks of age and median time to palpable tumor development is delayed by approximately 2 weeks

integument

decreased mammary gland tumor incidence ( J:208154 )

♀ • decreased development of spontaneous mammary tumor nodules at 14 weeks of age and median time to palpable tumor development is delayed by approximately 2 weeks