Phenotypes associated with this allele

• Allele Symbol: Runx1^tm2.1Gss
• Allele Name: targeted mutation 2.1, Gary S Stein
• Allele ID: MGI:5613812
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Runx1^tm2.1Gss/Runx1^tm2.1Gss	involves: 129S4/SvJae * C57BL/6	MGI:5614301

Genotype
MGI:5614301

hm1

• Allelic Composition: Runx1^tm2.1Gss/Runx1^tm2.1Gss
• Genetic Background: involves: 129S4/SvJae * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

mortality/aging ( J:214025 )

N • mice survive to adulthood

hematopoietic system

hematopoietic system phenotype ( J:214025 )

N • embryos and neonates exhibit no hematopoietic defects

N • mice exhibit normal thymus size and double negative T cell populations

N • the CD41+ compartments in the spleen and thymus are normal

enlarged spleen ( J:214025 )

• due to myeloid expansion or infiltration

abnormal definitive hematopoiesis ( J:214025 )

• hematopoietic progenitors from adult bone marrow exhibit increased proliferation ex vivo but decreased colony forming ability compared with wild-type cells

• however, cells remain sensitive to TGF-beta suppression of growth and common myeloid progenitors (CMP) populations are normal

myeloid hyperplasia ( J:214025 )

• expansion of splenic myeloid compartment

anemia ( J:214025 )

• subtle, subclinical

abnormal megakaryocyte morphology ( J:214025 )

• many smaller megakaryocytes with less nuclear hematoxylin staining

abnormal megakaryocyte differentiation ( J:214025 )

• delayed megakaryocyte maturation suggested by decrease in 8N and 16N cells

abnormal erythroid progenitor cell morphology ( J:214025 )

• bone marrow contains increased early erythroid progenitor populations but fewer mature red cell progenitors and reduced mature EryC progenitor cells compared to in wild-type mice

abnormal erythrocyte morphology ( J:214025 )

abnormal erythropoiesis ( J:214025 )

• delayed red blood cell maturation

• however, ProE cell populations are normal

increased erythrocyte cell number ( J:214025 )

decreased hematocrit ( J:214025 )

increased hemoglobin content ( J:214025 )

• increased hemoglobin concentration

decreased mean corpuscular volume ( J:214025 )

increased red blood cell distribution width ( J:214025 )

thrombocytosis ( J:214025 )

increased mean platelet volume ( J:214025 )

abnormal leukocyte morphology ( J:214025 )

decreased immature B cell number ( J:214025 )

• in the bone marrow

decreased pre-pro B cell number ( J:214025 )

increased pro-B cell number ( J:214025 )

increased granulocyte number ( J:214025 )

• mature granulocytes in the spleen but not the thymus

increased mature B cell number ( J:214025 )

• slightly in the bone marrow

decreased CD8-positive, alpha-beta T cell number ( J:214025 )

• in the spleen and thymus

increased macrophage cell number ( J:214025 )

• monocyte/macrophages in the spleen but not the thymus

increased monocyte cell number ( J:214025 )

• monocyte/macrophages in the spleen but not the thymus

abnormal spleen white pulp morphology ( J:214025 )

• decreased delineation of follicles and lower cell density

immune system

enlarged spleen ( J:214025 )

• due to myeloid expansion or infiltration

myeloid hyperplasia ( J:214025 )

• expansion of splenic myeloid compartment

abnormal leukocyte morphology ( J:214025 )

decreased immature B cell number ( J:214025 )

• in the bone marrow

decreased pre-pro B cell number ( J:214025 )

increased pro-B cell number ( J:214025 )

increased granulocyte number ( J:214025 )

• mature granulocytes in the spleen but not the thymus

increased mature B cell number ( J:214025 )

• slightly in the bone marrow

decreased CD8-positive, alpha-beta T cell number ( J:214025 )

• in the spleen and thymus

increased macrophage cell number ( J:214025 )

• monocyte/macrophages in the spleen but not the thymus

increased monocyte cell number ( J:214025 )

• monocyte/macrophages in the spleen but not the thymus

abnormal spleen white pulp morphology ( J:214025 )

• decreased delineation of follicles and lower cell density

cellular

abnormal megakaryocyte differentiation ( J:214025 )

• delayed megakaryocyte maturation suggested by decrease in 8N and 16N cells

growth/size/body

enlarged spleen ( J:214025 )

• due to myeloid expansion or infiltration