Phenotypes associated with this allele

• Allele Symbol: Cpap^{tm1d(EUCOMM)Wtsi}
• Allele Name: targeted mutation 1d, Wellcome Trust Sanger Institute
• Allele ID: MGI:5613963
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cpap^tm1d(EUCOMM)Wtsi/Cpap^tm1d(EUCOMM)Wtsi	involves: C57BL/6 * C57BL/6N * FVB/NJ	MGI:5609811
cn2	Cpap^tm1d(EUCOMM)Wtsi/Cpap^tm1c(EUCOMM)Wtsi Tg(Zp3-cre)93Knw/0	involves: C57BL/6J * C57BL/6N * FVB/NJ	MGI:5613975
cx3	Cpap^tm1d(EUCOMM)Wtsi/Cpap^tm1d(EUCOMM)Wtsi Trp53^tm1Tyj/Trp53^tm1Tyj	involves: 129S2/SvPas * C57BL/6 * C57BL/6N * FVB/NJ	MGI:5609812

Genotype
MGI:5609811

hm1

• Allelic Composition: Cpap^{tm1d(EUCOMM)Wtsi}/Cpap^{tm1d(EUCOMM)Wtsi}
• Genetic Background: involves: C57BL/6 * C57BL/6N * FVB/NJ
• Cell Lines: EPD0028_7_G05

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:208632 )

• arrest by E9.0

embryo

absent embryonic cilia ( J:208632 )

• absent at E7.5 and E8.5

• lack cilia and basal bodies but have normal planar cell polarity in node cells

increased embryonic tissue cell apoptosis ( J:208632 )

• embryos show many pyknotic and fragmented nuclei

abnormal embryo turning ( J:208632 )

• form a heart and neural tube but fail to undergo turning

embryonic growth arrest ( J:208632 )

• by E9.0

cellular

absent embryonic cilia ( J:208632 )

• absent at E7.5 and E8.5

• lack cilia and basal bodies but have normal planar cell polarity in node cells

abnormal centrosome morphology ( J:208632 )

• disrupted organization of the centrosome in embryonic cells

abnormal mitosis ( J:208632 )

• at E8.5 a 70% increase in the fraction of mitotic cells is seen

• prometaphase is prolonged in embryonic cells and the formation of defined spindle poles is delayed; however, in every mitosis the chromosome rosette resolves into 2 equal groups of chromosomes

increased embryonic tissue cell apoptosis ( J:208632 )

• embryos show many pyknotic and fragmented nuclei

Genotype
MGI:5613975

cn2

• Allelic Composition: Cpap^{tm1d(EUCOMM)Wtsi}/Cpap^{tm1c(EUCOMM)Wtsi}; Tg(Zp3-cre)93Knw/0
• Genetic Background: involves: C57BL/6J * C57BL/6N * FVB/NJ

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:208632 )

• arrest by E9.0

embryo

abnormal embryo development ( J:208632 )

• indistinguishable from germ line null mice

embryonic growth arrest ( J:208632 )

• arrest by E9.0

Genotype
MGI:5609812

cx3

• Allelic Composition: Cpap^{tm1d(EUCOMM)Wtsi}/Cpap^{tm1d(EUCOMM)Wtsi}; Trp53^tm1Tyj/Trp53^tm1Tyj
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * C57BL/6N * FVB/NJ
• Cell Lines: EPD0028_7_G05

embryo

embryo phenotype ( J:208632 )

N • survive until at least E9.5 and complete embryonic turning

abnormal dorsal-ventral axis patterning ( J:208632 )

• absence of ventral neural cell types that depend on high or intermediated SHH signaling levels and expansion of markers that are normally repressed by SHH into the ventral neural tube

abnormal neural tube morphology ( J:208632 )

• absence of ventral neural cell types that depend on high or intermediated SHH signaling levels and expansion of markers that are normally repressed by SHH into the ventral neural tube

abnormal embryonic neuroepithelium morphology ( J:208632 )

• lack centrioles at E8.5 even in mitotic cells in metaphase conformation

nervous system

abnormal neural tube morphology ( J:208632 )

• absence of ventral neural cell types that depend on high or intermediated SHH signaling levels and expansion of markers that are normally repressed by SHH into the ventral neural tube

abnormal embryonic neuroepithelium morphology ( J:208632 )

• lack centrioles at E8.5 even in mitotic cells in metaphase conformation

cellular

abnormal centrosome morphology ( J:208632 )

• disrupted organization of the centrosome in MEFs

abnormal mitosis ( J:208632 )

• disrupted organization of the centrosome in MEFs

• astral microtubules are present but decreased in number in MEFs

• no signs of increased aneuploidy are detected

abnormal apoptosis ( J:208632 )

• number of apoptotic cells is reduced compared to mice homozygous for Cenpj^{tm1b(EUCOMM)Wtsi} alone