Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxp3tm4(YFP/icre)Ayr mutation
(2 available);
any
Foxp3 mutation
(58 available)
Myd88tm1.1Medz mutation
(0 available);
any
Myd88 mutation
(52 available)
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immune system
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N |
• mice mount CD4+ T cell responses in response to immunization with OVA and LPS that are indistinguishable from wild-type mice
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Myd88tm1.1Medz mutation
(0 available);
any
Myd88 mutation
(52 available)
Tg(Cd4-cre)1Cwi mutation
(10 available)
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hematopoietic system
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• CD4+ T cells are impaired in their ability to proliferate and secrete IFN-gamma and IL-17 in response to immunization with ovalbumin (OVA) in the presence of lipopolysaccharide (LPS) followed by restimulation with OVA in the presence of irradiated splenocytes as antigen presenting cells
• CD4+ T cell response is defective in response to immunization with OVA plus either polyIC or CpG DNA
• mice depleted of CD25+ Treg cells (with a monoclonal anti-CD25 antibody) prior to immunization with OVA and LPS or OVA and CpG DNA are unable to restore the Th17 cell response, however the Th1 cell response is restored, with restoration of proliferation and secretion of IFN-gamma by restimulated CD4+ T cells
• however, clonal expansion of antigen-specific CD4+ T cells is not impaired
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• upon immunization with 2W peptide in the presence of LPS, the Th1 response is impaired, with the number of cells in the draining lymph nodes reduced compared to that of controls, leading to a reduction in the absolute number of CD4+ T cells specific for the 2W peptide
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• mice show absence of a Th17 cell response in response to immunization and restimulation
• however, mice do not exhibit an increased frequency of FoxP3+ Treg cells in unimmunized or immunized state, indicating normal numbers of iTreg cells
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• memory T cells are generated at similar frequencies to wild-type following immunization with OVA and LPS, however, CD4+ memory T cells are impaired in their ability to differentiate into IFN-gamma secreting T cells after secondary immunization, even under conditions where Treg cells are absent during both the primary and secondary immunization
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immune system
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• CD4+ T cells are impaired in their ability to proliferate and secrete IFN-gamma and IL-17 in response to immunization with ovalbumin (OVA) in the presence of lipopolysaccharide (LPS) followed by restimulation with OVA in the presence of irradiated splenocytes as antigen presenting cells
• CD4+ T cell response is defective in response to immunization with OVA plus either polyIC or CpG DNA
• mice depleted of CD25+ Treg cells (with a monoclonal anti-CD25 antibody) prior to immunization with OVA and LPS or OVA and CpG DNA are unable to restore the Th17 cell response, however the Th1 cell response is restored, with restoration of proliferation and secretion of IFN-gamma by restimulated CD4+ T cells
• however, clonal expansion of antigen-specific CD4+ T cells is not impaired
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• upon immunization with 2W peptide in the presence of LPS, the Th1 response is impaired, with the number of cells in the draining lymph nodes reduced compared to that of controls, leading to a reduction in the absolute number of CD4+ T cells specific for the 2W peptide
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• mice show absence of a Th17 cell response in response to immunization and restimulation
• however, mice do not exhibit an increased frequency of FoxP3+ Treg cells in unimmunized or immunized state, indicating normal numbers of iTreg cells
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• memory T cells are generated at similar frequencies to wild-type following immunization with OVA and LPS, however, CD4+ memory T cells are impaired in their ability to differentiate into IFN-gamma secreting T cells after secondary immunization, even under conditions where Treg cells are absent during both the primary and secondary immunization
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Analysis Tools