All Phenotypes Col1a1<tm2(tetO-IDH2*R140Q)Ppp> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Col1a1^{tm2(tetO-IDH2R140Q)Ppp}/Col1a1^{tm2(tetO-IDH2R140Q)Ppp} Flt3^tm1.1Dosm/Flt3^tm1.1Dosm Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae}/?	involves: 129 * C57BL/6	MGI:5697542
cn2	Col1a1^{tm2(tetO-IDH2R140Q)Ppp}/Col1a1^{tm2(tetO-IDH2R140Q)Ppp} Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae}/?	involves: 129S4/SvJae * C57BL/6	MGI:5697539

Allelic Composition	Col1a1^{tm2(tetO-IDH2R140Q)Ppp}/Col1a1^{tm2(tetO-IDH2R140Q)Ppp} Flt3^tm1.1Dosm/Flt3^tm1.1Dosm Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae}/?
Genetic Background	involves: 129 * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Col1a1^{tm2(tetO-IDH2*R140Q)Ppp} mutation (1 available); any Col1a1 mutation (163 available)
Flt3^tm1.1Dosm mutation (0 available); any Flt3 mutation (90 available)
Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} mutation (30 available); any Gt(ROSA)26Sor mutation (993 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

neoplasm

increased leukemia incidence ( J:210097 )

• mice treated with doxycycline develop acute leukemias

mortality/aging

premature death ( J:210097 )

• reduced overall survival of doxycycline treated mice to 229 days vs 352 days for Flt3^tm1.1Dosm controls

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

hematopoietic system phenotype ( J:210097 )

N	• modified IDH2 expression induced by doxycycline in chow starting around 3 weeks • peripheral blood cell counts, number of hematopoietic stem cells, myeloid progenitors, mature B and T lymphocytes, and myeloid cells all normal when examined 8 weeks after induction

abnormal hematopoietic stem cell proliferation ( J:210097 )

• KSL cells collected and plated out 8 weeks after induction display severely reduced erythroid colony formation but myeloid colony numbers are normal

• differentiation blockade is removed when doxycycline is removed from the culture medium

increased hematopoietic stem cell proliferation ( J:210097 )

• by 7 months KSL cells are increased in bone marrow relative to controls

• long-term hematopoietic stem cells also tend to expand in number

enlarged spleen ( J:210097 )

• about 1.3X larger than control spleens

extramedullary hematopoiesis ( J:210097 )

• significant extramedullary hematopoiesis

neoplasm

neoplasm ( J:210097 )

N	• no mice on doxycycline treatment for 1 year develop leukemia

increased leukemia incidence ( J:210097 )

• infection of KSL cells with retrovirus containing HoxA9 and Meis1 and then injected into sub-lethally irradiated mice results in a pre-leukemic cell expansion

cellular

abnormal hematopoietic stem cell proliferation ( J:210097 )

• KSL cells collected and plated out 8 weeks after induction display severely reduced erythroid colony formation but myeloid colony numbers are normal

• differentiation blockade is removed when doxycycline is removed from the culture medium

increased hematopoietic stem cell proliferation ( J:210097 )

• by 7 months KSL cells are increased in bone marrow relative to controls

• long-term hematopoietic stem cells also tend to expand in number

immune system

enlarged spleen ( J:210097 )

• about 1.3X larger than control spleens

growth/size/body

enlarged spleen ( J:210097 )

• about 1.3X larger than control spleens

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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