Analysis Tools
mortality/aging
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• mice die shortly after birth
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respiratory system
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• mice exhibit unexpanded lungs upon postmortem autopsy
• however, lungs exhibit no abnormalities
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nervous system
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• the central neural respiratory network located in the brainstem, which includes cranial motor neurons of the nucleus ambiguous, is absent at E15.5 and E18.5
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• disorganization of the neurons that innervate facial muscles
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• the central neural respiratory network located in the brainstem, which includes cranial motor neurons of the nucleus ambiguous, is absent at E15.5 and E18.5
• mice exhibit fewer motor neurons in the cervical spinal cord at levels C3-C5
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• phrenic nerve exhibits fewer axonal branches
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cellular
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• motile mitochondria in cortical axons spend less time in motion compared to wild-type mitochondria
• mitochondria in axons exhibits a 4-fold reduction in overall retrograde velocity, however, anterograde velocity is no different
• 2-fold reduction in retrograde run length in cortical neurons
indicates, that retrograde axonal mitochondrial movement is affected
• mitochondrial respiration is intact and mitochondrial membrane potential is not altered
• mitochondria in MEFs is clustered around the nucleus and is absent from filopodia unlike in wild-type MEFs which show distribution throughout the cytoplasm and in filopodia
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homeostasis/metabolism
cardiovascular system
| N |
• mice exhibit normal cardiac function before birth as indicated by normal fetal cardiac ultrasound at E18.5
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