Phenotypes associated with this allele

• Allele Symbol: Snai3^tm1.2Jhws
• Allele Name: targeted mutation 1.2, John Weis
• Allele ID: MGI:5620192
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Snai3^tm1.2Jhws/Snai3^tm1.2Jhws	involves: 129/Sv * 129S1/Sv	MGI:5620193
cx2	Snai2^tm2Grid/Snai2^tm2Grid Snai3^tm1.2Jhws/Snai3^tm1.2Jhws	involves: 129/Sv * 129S1/Sv * C57BL/6	MGI:5620202
cx3	Snai2^tm2Grid/Snai2^+ Snai3^tm1.2Jhws/Snai3^tm1.2Jhws	involves: 129/Sv * 129S1/Sv * C57BL/6	MGI:5620264
cx4	Snai2^tm2Grid/Snai2^tm2Grid Snai3^tm1.2Jhws/Snai3^+	involves: 129/Sv * 129S1/Sv * C57BL/6	MGI:5620265

Genotype
MGI:5620193

hm1

• Allelic Composition: Snai3^tm1.2Jhws/Snai3^tm1.2Jhws
• Genetic Background: involves: 129/Sv * 129S1/Sv

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:204710 )

• mice are viable and fertile and display no apparent developmental defects

Genotype
MGI:5620202

cx2

• Allelic Composition: Snai2^tm2Grid/Snai2^tm2Grid; Snai3^tm1.2Jhws/Snai3^tm1.2Jhws
• Genetic Background: involves: 129/Sv * 129S1/Sv * C57BL/6

mortality/aging

lethality, incomplete penetrance ( J:204710 )

• approximately 50% fewer homozygotes are produced than expected from heterozygote x heterozygote matings

• fewer homozygote females are produced than expected from heterozygote x heterozygote matings

premature death ( J:204710 )

• few mice survive beyond 15 weeks of age

growth/size/body

decreased body weight ( J:204710 )

• mice are approximately half the size and weight of controls

hematopoietic system

abnormal thymus cortex morphology ( J:204710 )

• altered organization of thymic cortex

abnormal thymus medulla morphology ( J:204710 )

• altered organization of thymic medulla

small thymus ( J:204710 )

• thymus is smaller than wild-type controls

thymus fibrosis ( J:204710 )

• thymus is fibrotic

abnormal thymus involution ( J:204710 )

• thymus is mostly involuted

decreased double-positive T cell number ( J:204710 )

• reduced numbers of thymic CD4+ CD8+ cells

increased neutrophil cell number ( J:204710 )

• increased neutrophil (Cd11b+ Gr1^Hi) population in bone marrow, spleen and blood

decreased B cell number ( J:204710 )

• decrease in numbers of B220+ CD19+ cells in bone marrow

• reduced numbers of B220+ CD19+ cells in the blood; phenotype is more severe in comparison to that observed in Snai3^+/-Snai2^-/- and Snai3^-/-Snai2^+/- mice

• reduced numbers of B220+ CD19+ cells in the spleen

increased single-positive T cell number ( J:204710 )

• increase in CD4+ single positive cells

increased macrophage cell number ( J:204710 )

• increased macrophage (Cd11b+ Gr1^Int) population in bone marrow and spleen

small spleen ( J:204710 )

• spleen is approximately one third the size of heterozygote controls at 4 weeks of age

disorganized spleen B cell follicle ( J:204710 )

• extensive follicle disorganization is observed in spleens from 6 week old animals

• phenotype is more severe in comparison to Snai3^+/-Snai2^-/- and Snai3^-/-Snai2^+/-

• spleen follicles are ringed with a lighter, cell deficient zone

immune system

abnormal thymus cortex morphology ( J:204710 )

• altered organization of thymic cortex

abnormal thymus medulla morphology ( J:204710 )

• altered organization of thymic medulla

small thymus ( J:204710 )

• thymus is smaller than wild-type controls

thymus fibrosis ( J:204710 )

• thymus is fibrotic

abnormal thymus involution ( J:204710 )

• thymus is mostly involuted

decreased double-positive T cell number ( J:204710 )

• reduced numbers of thymic CD4+ CD8+ cells

increased neutrophil cell number ( J:204710 )

• increased neutrophil (Cd11b+ Gr1^Hi) population in bone marrow, spleen and blood

decreased B cell number ( J:204710 )

• decrease in numbers of B220+ CD19+ cells in bone marrow

• reduced numbers of B220+ CD19+ cells in the blood; phenotype is more severe in comparison to that observed in Snai3^+/-Snai2^-/- and Snai3^-/-Snai2^+/- mice

• reduced numbers of B220+ CD19+ cells in the spleen

increased single-positive T cell number ( J:204710 )

• increase in CD4+ single positive cells

increased macrophage cell number ( J:204710 )

• increased macrophage (Cd11b+ Gr1^Int) population in bone marrow and spleen

small spleen ( J:204710 )

• spleen is approximately one third the size of heterozygote controls at 4 weeks of age

disorganized spleen B cell follicle ( J:204710 )

• extensive follicle disorganization is observed in spleens from 6 week old animals

• phenotype is more severe in comparison to Snai3^+/-Snai2^-/- and Snai3^-/-Snai2^+/-

• spleen follicles are ringed with a lighter, cell deficient zone

endocrine/exocrine glands

abnormal thymus cortex morphology ( J:204710 )

• altered organization of thymic cortex

abnormal thymus medulla morphology ( J:204710 )

• altered organization of thymic medulla

small thymus ( J:204710 )

• thymus is smaller than wild-type controls

thymus fibrosis ( J:204710 )

• thymus is fibrotic

abnormal thymus involution ( J:204710 )

• thymus is mostly involuted

reproductive system

male infertility ( J:204710 )

♂ • males do not produce offspring

vision/eye

abnormal eye morphology ( J:204710 )

• eye abnormalities are observed

Genotype
MGI:5620264

cx3

• Allelic Composition: Snai2^tm2Grid/Snai2⁺; Snai3^tm1.2Jhws/Snai3^tm1.2Jhws
• Genetic Background: involves: 129/Sv * 129S1/Sv * C57BL/6

endocrine/exocrine glands

abnormal thymus cortex morphology ( J:204710 )

• altered thymic cortex organization

abnormal thymus medulla morphology ( J:204710 )

• altered thymic medulla organization

hematopoietic system

abnormal thymus cortex morphology ( J:204710 )

• altered thymic cortex organization

abnormal thymus medulla morphology ( J:204710 )

• altered thymic medulla organization

decreased B cell number ( J:204710 )

• reduced numbers of B220+ CD19+ cells in the blood

• phenotype is less severe than that observed in Snai3^-/-Snai2^-/- and Snai3^-/+Snai2^-/- mice

disorganized spleen B cell follicle ( J:204710 )

• follicle disorganization observed in spleens from 6 week old animals

• phenotype is less severe than that observed in Snai3^-/-Snai2^-/- mice

immune system

abnormal thymus cortex morphology ( J:204710 )

• altered thymic cortex organization

abnormal thymus medulla morphology ( J:204710 )

• altered thymic medulla organization

decreased B cell number ( J:204710 )

• reduced numbers of B220+ CD19+ cells in the blood

• phenotype is less severe than that observed in Snai3^-/-Snai2^-/- and Snai3^-/+Snai2^-/- mice

disorganized spleen B cell follicle ( J:204710 )

• follicle disorganization observed in spleens from 6 week old animals

• phenotype is less severe than that observed in Snai3^-/-Snai2^-/- mice

Genotype
MGI:5620265

cx4

• Allelic Composition: Snai2^tm2Grid/Snai2^tm2Grid; Snai3^tm1.2Jhws/Snai3⁺
• Genetic Background: involves: 129/Sv * 129S1/Sv * C57BL/6

endocrine/exocrine glands

abnormal thymus cortex morphology ( J:204710 )

• altered thymic cortex organization

abnormal thymus medulla morphology ( J:204710 )

• altered thymic medulla organization

hematopoietic system

abnormal thymus cortex morphology ( J:204710 )

• altered thymic cortex organization

abnormal thymus medulla morphology ( J:204710 )

• altered thymic medulla organization

increased neutrophil cell number ( J:204710 )

• increase in number of neutrophils (Cd11b+ Gr1^Hi) in the blood from 6 month old mice

decreased B cell number ( J:204710 )

• reduced numbers of B220+ CD19+ cells in the blood

• phenotype is intermediate between Snai3^-/-Snai2^-/- and Snai3^-/-Snai2^+/-

increased macrophage cell number ( J:204710 )

• increase in number of macrophages (Cd11b+ Gr1^Int) in the blood from 6 month old mice

disorganized spleen B cell follicle ( J:204710 )

• follicle disorganization observed in spleens from 6 week old animals, however, phenotype is less severe than that observed in Snai3^-/-Snai2^-/- mice

immune system

abnormal thymus cortex morphology ( J:204710 )

• altered thymic cortex organization

abnormal thymus medulla morphology ( J:204710 )

• altered thymic medulla organization

increased neutrophil cell number ( J:204710 )

• increase in number of neutrophils (Cd11b+ Gr1^Hi) in the blood from 6 month old mice

decreased B cell number ( J:204710 )

• reduced numbers of B220+ CD19+ cells in the blood

• phenotype is intermediate between Snai3^-/-Snai2^-/- and Snai3^-/-Snai2^+/-

increased macrophage cell number ( J:204710 )

• increase in number of macrophages (Cd11b+ Gr1^Int) in the blood from 6 month old mice

disorganized spleen B cell follicle ( J:204710 )

• follicle disorganization observed in spleens from 6 week old animals, however, phenotype is less severe than that observed in Snai3^-/-Snai2^-/- mice