All Phenotypes Nus1<tm1Wcsa> MGI Mouse

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Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

Nus1^tm1Wcsa
targeted mutation 1, William C Sessa
MGI:5620526

Summary

5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Nus1^tm1Wcsa/Nus1^tm1Wcsa	Not Specified	MGI:6361102
cn2	Nus1^tm1Wcsa/Nus1^tm1.1Wcsa	involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ	MGI:5620602
cn3	Nus1^tm1Wcsa/Nus1⁺ Rtn4^tm1Matl/Rtn4^tm1Matl Tg(Cdh5-cre/ERT2)1Rha/0	involves: 129S7/SvEvBrd	MGI:6361104
cn4	Nus1^tm1Wcsa/Nus1^tm1Wcsa Tg(Tek-cre)12Flv/0	involves: C3H * C57BL/6	MGI:6361094
cn5	Nus1^tm1Wcsa/Nus1^tm1Wcsa Tg(Cdh5-cre/ERT2)1Rha/0	Not Specified	MGI:6361095

Allelic Composition	Nus1^tm1Wcsa/Nus1^tm1Wcsa
Genetic Background	Not Specified

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nus1^tm1Wcsa mutation (0 available); any Nus1 mutation (19 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

cellular

increased lung endothelial cell apoptosis ( J:230506 )

• lung endothelial cells treated with Ad-Cre show increased apoptosis

• dolichol phosphate supplementation in the media of lung endothelial cell cultures partially rescues the apoptosis defects

decreased lung endothelial cell proliferation ( J:230506 )

• lung endothelial cells treated with an adenovirus-expressing cre recombinase show reduced proliferation

• dolichol phosphate supplementation in the media of lung endothelial cell cultures partially rescues the proliferation defects

respiratory system

abnormal lung endothelial cell physiology ( J:230506 )

• total dolichol levels in lung endothelial cells treated with an adenovirus-expressing cre (Ad-Cre) recombinase are reduced

• lung endothelial cells show reduced glycosylation of VEGFR2, CD31, and VE-cad and increased ER stress markers

• dolichol phosphate supplementation in the media of lung endothelial cell cultures increases the levels of glycosylated endothelial proteins, indicating rescue of glycosylation defects

increased lung endothelial cell apoptosis ( J:230506 )

• lung endothelial cells treated with Ad-Cre show increased apoptosis

• dolichol phosphate supplementation in the media of lung endothelial cell cultures partially rescues the apoptosis defects

decreased lung endothelial cell proliferation ( J:230506 )

• lung endothelial cells treated with an adenovirus-expressing cre recombinase show reduced proliferation

• dolichol phosphate supplementation in the media of lung endothelial cell cultures partially rescues the proliferation defects

Allelic Composition	Nus1^tm1Wcsa/Nus1^tm1.1Wcsa
Genetic Background	involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nus1^tm1.1Wcsa mutation (0 available); any Nus1 mutation (19 available)
Nus1^tm1Wcsa mutation (0 available); any Nus1 mutation (19 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

cellular

abnormal cell physiology ( J:215785 )

• tamoxifen-treated mouse embryonic fibroblasts exhibit decreased cis-PTase activity in isolated membranes and mannose incorporation into proteins compared with wild-type cells

increased sensitivity to induced cell death ( J:215785 )

• in tamoxifen-treated mouse embryonic fibroblasts exposed to lovastatin

abnormal cellular cholesterol metabolism ( J:215785 )

• tamoxifen-treated mouse embryonic fibroblasts accumulate free cholesterol compared with control mice

homeostasis/metabolism

abnormal cellular cholesterol metabolism ( J:215785 )

• tamoxifen-treated mouse embryonic fibroblasts accumulate free cholesterol compared with control mice

Allelic Composition	Nus1^tm1Wcsa/Nus1⁺ Rtn4^tm1Matl/Rtn4^tm1Matl Tg(Cdh5-cre/ERT2)1Rha/0
Genetic Background	involves: 129S7/SvEvBrd

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nus1^tm1Wcsa mutation (0 available); any Nus1 mutation (19 available)
Rtn4^tm1Matl mutation (1 available); any Rtn4 mutation (131 available)
Tg(Cdh5-cre/ERT2)1Rha mutation (4 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

cardiovascular system

cardiovascular system phenotype ( J:230506 )

N	• embryos from tamoxifen-treated pregnant females at E8.5 do not show vascular defects

Allelic Composition	Nus1^tm1Wcsa/Nus1^tm1Wcsa Tg(Tek-cre)12Flv/0
Genetic Background	involves: C3H * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nus1^tm1Wcsa mutation (0 available); any Nus1 mutation (19 available)
Tg(Tek-cre)12Flv mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:230506 )

• lethality between E10.5 and E11.5

growth/size/body

decreased embryo size ( J:230506 )

• embryos are smaller at E10.5 but not at E8.5 or E9.5

embryo

abnormal placenta vasculature ( J:230506 )

• placentas show dilated embryonic vessels and decreased numbers of embryonic vessels

abnormal vitelline vasculature morphology ( J:230506 )

• yolk sacs are poorly organized with dilated primitive capillaries and have no large vitelline vessels

abnormal embryo morphology ( J:230506 )

• E10.5 embryos are paler than controls

decreased embryo size ( J:230506 )

• embryos are smaller at E10.5 but not at E8.5 or E9.5

thin placenta labyrinth ( J:230506 )

• the labyrinthine layers of the placenta are thinner

excessive folding of visceral yolk sac ( J:230506 )

• yolk sacs are dimpled and wrinkled at E9.5

abnormal visceral yolk sac physiology ( J:230506 )

• yolk sacs at E9.5 show 39% reductions in endothelial cell proliferation

• yolk sacs at E9.5 show increased apoptosis of endothelial cells

cardiovascular system

abnormal placenta vasculature ( J:230506 )

• placentas show dilated embryonic vessels and decreased numbers of embryonic vessels

abnormal vitelline vasculature morphology ( J:230506 )

• yolk sacs are poorly organized with dilated primitive capillaries and have no large vitelline vessels

abnormal vascular endothelial cell physiology ( J:230506 )

• hypoglycosylation of endothelial proteins including VEGFR2

Allelic Composition	Nus1^tm1Wcsa/Nus1^tm1Wcsa Tg(Cdh5-cre/ERT2)1Rha/0
Genetic Background	Not Specified

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nus1^tm1Wcsa mutation (0 available); any Nus1 mutation (19 available)
Tg(Cdh5-cre/ERT2)1Rha mutation (4 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

embryonic lethality, complete penetrance ( J:230506 )

• embryos from tamoxifen-treated females at E8.5 and E9.5 die 5 days after tamoxifen injection

embryo

abnormal vitelline vasculature morphology ( J:230506 )

• yolk sacs show vascular defects in embryos from tamoxifen-treated females at E8.5 and E9.5

abnormal jugular lymph sac morphology ( J:230506 )

• embryos from tamoxifen-treated females at E8.5 and E9.5 show blood filled jugular lymph sacs at E13.5

cardiovascular system

abnormal vascular development ( J:230506 )

• embryos from tamoxifen-treated females at E8.5 and E9.5 show dilation of subcutaneous microvessels and blood filled jugular lymph sacs at E13.

• reduction in vascular density and disorganized vascular networks are seen in embryonic tissues (hindbrain, skin, and hindlimb) of embryos from tamoxifen-treated females at E8.5 and E9.5

abnormal vitelline vasculature morphology ( J:230506 )

• yolk sacs show vascular defects in embryos from tamoxifen-treated females at E8.5 and E9.5

hemorrhage ( J:230506 )

• embryos from tamoxifen-treated females at E8.5 and E9.5 exhibit extensive multifocal subcutaneous hemorrhages at E12.5 and E13.5

increased vascular endothelial cell apoptosis ( J:230506 )

• dorsal skin of embryos from tamoxifen-treated females show increased apoptosis

cellular

increased vascular endothelial cell apoptosis ( J:230506 )

• dorsal skin of embryos from tamoxifen-treated females show increased apoptosis

decreased endothelial cell proliferation ( J:230506 )

• embryos from tamoxifen-treated females at E8.5 and E9.5 show 48% reductions in endothelial cell proliferation at E12.5

homeostasis/metabolism

subcutaneous edema ( J:230506 )

• embryos from tamoxifen-treated females at E8.5 and E9.5 exhibit subcutaneous edema

immune system

abnormal jugular lymph sac morphology ( J:230506 )

• embryos from tamoxifen-treated females at E8.5 and E9.5 show blood filled jugular lymph sacs at E13.5

integument

subcutaneous edema ( J:230506 )

• embryos from tamoxifen-treated females at E8.5 and E9.5 exhibit subcutaneous edema

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