Phenotypes associated with this allele

• Allele Symbol: Fitm2^tm1.1Dls
• Allele Name: targeted mutation 1.1, David L Silver
• Allele ID: MGI:5621176
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Fitm2^tm1.1Dls/Fitm2^tm1.1Dls Tg(Fabp4-cre)1Rev/?	involves: 129S6/SvEvTac * C57BL/6J	MGI:5707239
cn2	Fitm2^tm1.1Dls/Fitm2^tm1.1Dls Lep^ob/Lep^ob Tg(Fabp4-cre)1Rev/?	involves: 129S6/SvEvTac * C57BL/6J	MGI:5707240

Genotype
MGI:5707239

cn1

• Allelic Composition: Fitm2^tm1.1Dls/Fitm2^tm1.1Dls; Tg(Fabp4-cre)1Rev/?
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Reduced amounts of subcutaneous white adipose tissue and epididmyal white adipose tissue in Fitm2^tm1.1Dls/Fitm2^tm1.1Dls Tg(Fabp4-cre)1Rev/? mice

growth/size/body

growth/size/body region phenotype ( J:212437 )

N • no change in body weight gain over time

increased body weight ( J:212437 )

• weight gain after 10-12 weeks on a high fat diet

increased liver weight ( J:212437 )

• livers weigh 25% more than in controls at 24 weeks of age

• increased liver weights after 12 weeks on a high fat diet

behavior/neurological

behavior/neurological phenotype ( J:212437 )

N • normal food intake

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:212437 )

N • normal plasma leptin levels

N • normal glucose and insulin tolerance

increased circulating tumor necrosis factor level ( J:212437 )

• after 12 weeks on a high fat diet

abnormal glucose homeostasis ( J:212437 )

increased circulating insulin level ( J:212437 )

• hyperinsulinemia at 24 weeks of age

• also after 12 weeks on a high fat diet

insulin resistance ( J:212437 )

• insulin intolerance at 24 weeks of age

abnormal fatty acids level ( J:212437 )

• higher basal and stimulated fatty acid release relative to controls after 12 weeks on a high fat diet

adipose tissue

abnormal brown fat cell morphology ( J:212437 )

increased brown fat cell lipid droplet size ( J:212437 )

decreased brown fat lipid droplet number ( J:212437 )

abnormal white adipose tissue morphology ( J:212437 )

• decreased white adipose tissue at 24 weeks of age

• decreased white adipose tissue after 12 weeks on a high fat diet

• macrophage infiltration of epididymal white adipose tissue

abnormal white fat cell morphology ( J:212437 )

• heterogeneity in adipocyte size after 12 weeks on a high fat diet

• lipid droplets significantly larger 4 days after adipocyte differentiation

• fewer lipid droplets than in controls

abnormal white adipose tissue physiology ( J:212437 )

• massive accumulation of macrophage after 12 weeks on a high fat diet

• indicative of increased adipocyte death

liver/biliary system

increased liver weight ( J:212437 )

• livers weigh 25% more than in controls at 24 weeks of age

• increased liver weights after 12 weeks on a high fat diet

pale liver ( J:212437 )

• after 12 weeks on a high fat diet

immune system

increased circulating tumor necrosis factor level ( J:212437 )

• after 12 weeks on a high fat diet

Genotype
MGI:5707240

cn2

• Allelic Composition: Fitm2^tm1.1Dls/Fitm2^tm1.1Dls; Lep^ob/Lep^ob; Tg(Fabp4-cre)1Rev/?
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6J

Reduced adiposity and unilocular lipid droplets in Fitm2^tm1.1Dls/Fitm2^tm1.1Dls Tg(Fabp4-cre)1Rev/? Lep^ob/Lep^ob mice

growth/size/body

decreased body weight ( J:212437 )

• reduced body weight at 10 weeks relative to homozygous Lep^ob

adipose tissue

decreased subcutaneous adipose tissue amount ( J:212437 )

• at 10 weeks relative to homozygous Lep^ob

decreased white adipose tissue amount ( J:212437 )

• reduced epididymal white adipose tissue at 10 weeks relative to homozygous Lep^ob

• increased macrophage infiltration

integument

decreased subcutaneous adipose tissue amount ( J:212437 )

• at 10 weeks relative to homozygous Lep^ob