About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Pip4k2ctm1b(KOMP)Wtsi
targeted mutation 1b, Wellcome Trust Sanger Institute
MGI:5629320
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Pip4k2ctm1b(KOMP)Wtsi/Pip4k2ctm1b(KOMP)Wtsi C57BL/6N-Pip4k2ctm1b(KOMP)Wtsi/J MGI:6263165
hm2
Pip4k2ctm1b(KOMP)Wtsi/Pip4k2ctm1b(KOMP)Wtsi involves: C57BL/6J * C57BL/6N MGI:5817355


Genotype
MGI:6263165
hm1
Allelic
Composition
Pip4k2ctm1b(KOMP)Wtsi/Pip4k2ctm1b(KOMP)Wtsi
Genetic
Background
C57BL/6N-Pip4k2ctm1b(KOMP)Wtsi/J
Cell Lines EPD0383_6_B01
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pip4k2ctm1b(KOMP)Wtsi mutation (1 available); any Pip4k2c mutation (32 available)
Data Sources
phenotype observed in females
phenotype observed in males
N normal phenotype
behavior/neurological
IMPC - JAX

hearing/vestibular/ear

homeostasis/metabolism




Genotype
MGI:5817355
hm2
Allelic
Composition
Pip4k2ctm1b(KOMP)Wtsi/Pip4k2ctm1b(KOMP)Wtsi
Genetic
Background
involves: C57BL/6J * C57BL/6N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pip4k2ctm1b(KOMP)Wtsi mutation (1 available); any Pip4k2c mutation (32 available)
phenotype observed in females
phenotype observed in males
N normal phenotype

Immune infiltrates in the liver from Pip4k2ctm1b(KOMP)Wtsi/Pip4k2ctm1b(KOMP)Wtsi mice

cellular
• in culture, CD4+ T cells isolated from spleen are hypersensitive to anti-CD3 plus anti-CD28 stimulated proliferation, as measured by 3H-thymidine incorporation

immune system
• increased CD4+ T cell number in spleen at 12-14 months of age
• increased CD8+ T cell number in spleen at 12-14 months of age
• decreased number of Foxp3+CD4+ Treg cells in spleen at 12-14 months of age
• increased plasma IgG3 levels at 12 months of age, consistent with increased T cell-derived IL-17 levels
• at 12-14 months of age, mice exhibit an increase in CD44+ active T cells and a decrease in CD62L+ naive T cells, suggesting that T cells are hyperactivated
• in culture, CD4+ T cells isolated from spleen are hypersensitive to anti-CD3 plus anti-CD28 stimulated proliferation, as measured by 3H-thymidine incorporation
• increased plasma MCP-1 levels at 12-14 months of age
• increased plasma interferon-gamma levels at 12-14 months of age
• plasma interferon-gamma levels are somewhat reduced at 24 h of rapamycin treatment and return to basal (pre-treatment) levels after 2 weeks of rapamycin treatment, similar to what is observed in wild-type controls
• increased plasma IL-10 levels at 12-14 months of age
• increased plasma IL-12 levels at 12-14 months of age
• plasma IL-12(p70) levels are not significantly altered in response to rapamycin treatment, similar to what is observed in wild-type controls
• increased plasma levels of IL-12(p40) levels at 12-14 months of age
• plasma levels of IL-12(p40) are reduced to wild-type levels at 24 h of rapamycin treatment and remain suppressed after 2 weeks of treatment; in contrast, rapamycin has no significant effect on plasma IL-12(p40) in wild-type controls
• increased plasma IL-2 levels at 12-14 months of age
• plasma IL-2 levels are not significantly altered in response to rapamycin treatment, similar to what is observed in wild-type controls
• increased plasma IL-4 levels at 12-14 months of age
• increased interferon-gamma secretion by CD4+ T cells isolated from spleen
• increased IL-17 secretion by CD4+ T cells isolated from spleen
• ratio of immune infiltrates per total area is significantly increased in liver tissue, indicating chronic inflammation without a specific trigger such as infection or injury
• mice develop enhanced immune responses resulting in autoimmunity
• mammalian target of rapamycin complex 1 (mTORC1) signaling is highly activated in several tissues
• hyperimmune phenotype can be partially ameliorated by treatment with rapamycin, the allosteric mTORC1 inhibitor
• immune cell infiltration in the intestine at 12-14 months of age
• immune cell infiltration in the salivary gland at 12-14 months of age
• immune cell infiltration in the liver at 12-14 months of age
• infiltrating immune cells in liver tissue are predominantly CD3+ T cells and B220+ B cells
• ratio of immune infiltrates per total area is significantly increased in liver tissue, indicating chronic inflammation
• area of immune infiltrates in liver is dramatically reduced after 2 weeks of rapamycin treatment
• immune cell infiltration in the kidney at 12-14 months of age
• immune cell infiltration in the lungs at 12-14 months of age

homeostasis/metabolism
N
• mice do not exhibit enhanced insulin sensitivity and are not protected from obesity on a high-fat diet
• decreased blood urea nitrogen levels at 12 months of age
• increased plasma VEGF levels at 12-14 months of age
• increased plasma MCP-1 levels at 12-14 months of age
• increased plasma interferon-gamma levels at 12-14 months of age
• plasma interferon-gamma levels are somewhat reduced at 24 h of rapamycin treatment and return to basal (pre-treatment) levels after 2 weeks of rapamycin treatment, similar to what is observed in wild-type controls
• increased plasma IL-10 levels at 12-14 months of age
• increased plasma IL-12 levels at 12-14 months of age
• plasma IL-12(p70) levels are not significantly altered in response to rapamycin treatment, similar to what is observed in wild-type controls
• increased plasma levels of IL-12(p40) levels at 12-14 months of age
• plasma levels of IL-12(p40) are reduced to wild-type levels at 24 h of rapamycin treatment and remain suppressed after 2 weeks of treatment; in contrast, rapamycin has no significant effect on plasma IL-12(p40) in wild-type controls
• increased plasma IL-2 levels at 12-14 months of age
• plasma IL-2 levels are not significantly altered in response to rapamycin treatment, similar to what is observed in wild-type controls
• increased plasma IL-4 levels at 12-14 months of age
• increased plasma aspartate transaminase (AST) levels at 12 months of age
• however, plasma alanine transaminase (ALT) levels are normal

hematopoietic system
• increased CD4+ T cell number in spleen at 12-14 months of age
• increased CD8+ T cell number in spleen at 12-14 months of age
• decreased number of Foxp3+CD4+ Treg cells in spleen at 12-14 months of age
• increased plasma IgG3 levels at 12 months of age, consistent with increased T cell-derived IL-17 levels
• at 12-14 months of age, mice exhibit an increase in CD44+ active T cells and a decrease in CD62L+ naive T cells, suggesting that T cells are hyperactivated
• in culture, CD4+ T cells isolated from spleen are hypersensitive to anti-CD3 plus anti-CD28 stimulated proliferation, as measured by 3H-thymidine incorporation

liver/biliary system
• immune cell infiltration in the liver at 12-14 months of age
• infiltrating immune cells in liver tissue are predominantly CD3+ T cells and B220+ B cells
• ratio of immune infiltrates per total area is significantly increased in liver tissue, indicating chronic inflammation
• area of immune infiltrates in liver is dramatically reduced after 2 weeks of rapamycin treatment
• pale livers are occasionally seen in mice at >12 months of age

digestive/alimentary system
• immune cell infiltration in the intestine at 12-14 months of age
• immune cell infiltration in the salivary gland at 12-14 months of age

renal/urinary system
• immune cell infiltration in the kidney at 12-14 months of age

respiratory system
• immune cell infiltration in the lungs at 12-14 months of age

endocrine/exocrine glands
• immune cell infiltration in the salivary gland at 12-14 months of age

growth/size/body
N
• mice develop normally and grow into adulthood with no obvious growth abnormalities





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
11/12/2024
MGI 6.24
The Jackson Laboratory