Phenotypes associated with this allele

• Allele Symbol: Prepl^tm1.2Sagh
• Allele Name: targeted mutation 1.2, Alan Saghatelian
• Allele ID: MGI:5629515
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Prepl^tm1.2Sagh/Prepl^tm1.2Sagh	involves: 129S4/SvJae * C57BL/6 * C57BL/6J * Swiss Webster	MGI:5662459

Genotype
MGI:5662459

hm1

• Allelic Composition: Prepl^tm1.2Sagh/Prepl^tm1.2Sagh
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * C57BL/6J * Swiss Webster

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

decreased body size ( J:213429 )

• at 1 month of age, homozygotes are visibly smaller than wild-type controls

decreased body weight ( J:213429 )

• at ~P20, both female and male homozygotes exhibit lower weights than wild-type controls; this trend is continued and increased up to P40

• extended growth curve analysis between 1 and 26 weeks of age revealed that homozygotes continue to lag behind in weight later in life, with final weights being significantly lower than those of wild-type controls

slow postnatal weight gain ( J:213429 )

• female homozygotes begin to gain less weight than controls at ~3 weeks of age, with the data being statistically significant at P26

• male homozygotes exhibit significantly less weight gain than controls from P21

decreased body length ( J:213429 )

• at 1 month of age, homozygotes display shorter body lengths than wild-type controls

postnatal growth retardation ( J:213429 )

• at 1 month of age, both female and male homozygotes exhibit diminished growth rates relative to wild-type controls

• extended growth curve analysis between 1 and 21 weeks of age revealed that homozygotes are smaller than wild-type controls and this difference in size is maintained throughout their lifetimes

behavior/neurological

impaired righting response ( J:213429 )

• in a surface righting assay, neonatal (P5) homozygotes take over twice as long to assume the normal upright position with all four paws on the ground as wild-type controls

weakness ( J:213429 )

• at 6 months of age, homozygotes show no difference in grip strength relative to wild-type controls, when normalized for body weight; however, because of their smaller size, homozygotes are not as strong on an absolute scale

muscle

hypotonia ( J:213429 )

• at P5, homozygotes display neonatal hypotonia, as determined by a surface righting assay